Job ID = 14520933 SRX = SRX8245446 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15439543 spots for SRR11684657/SRR11684657.sra Written 15439543 spots for SRR11684657/SRR11684657.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:23 15439543 reads; of these: 15439543 (100.00%) were paired; of these: 7353093 (47.63%) aligned concordantly 0 times 7473716 (48.41%) aligned concordantly exactly 1 time 612734 (3.97%) aligned concordantly >1 times ---- 7353093 pairs aligned concordantly 0 times; of these: 419143 (5.70%) aligned discordantly 1 time ---- 6933950 pairs aligned 0 times concordantly or discordantly; of these: 13867900 mates make up the pairs; of these: 7655975 (55.21%) aligned 0 times 5594852 (40.34%) aligned exactly 1 time 617073 (4.45%) aligned >1 times 75.21% overall alignment rate Time searching: 00:08:23 Overall time: 00:08:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 218965 / 8504529 = 0.0257 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:27:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:27:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:27:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:27:43: 1000000 INFO @ Sat, 15 Jan 2022 20:27:48: 2000000 INFO @ Sat, 15 Jan 2022 20:27:53: 3000000 INFO @ Sat, 15 Jan 2022 20:27:58: 4000000 INFO @ Sat, 15 Jan 2022 20:28:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:08: 6000000 INFO @ Sat, 15 Jan 2022 20:28:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:13: 7000000 INFO @ Sat, 15 Jan 2022 20:28:14: 1000000 INFO @ Sat, 15 Jan 2022 20:28:19: 8000000 INFO @ Sat, 15 Jan 2022 20:28:20: 2000000 INFO @ Sat, 15 Jan 2022 20:28:25: 9000000 INFO @ Sat, 15 Jan 2022 20:28:26: 3000000 INFO @ Sat, 15 Jan 2022 20:28:30: 10000000 INFO @ Sat, 15 Jan 2022 20:28:32: 4000000 BedGraph に変換中... INFO @ Sat, 15 Jan 2022 20:28:36: 11000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:37: 5000000 INFO @ Sat, 15 Jan 2022 20:28:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:42: 12000000 INFO @ Sat, 15 Jan 2022 20:28:43: 6000000 INFO @ Sat, 15 Jan 2022 20:28:44: 1000000 INFO @ Sat, 15 Jan 2022 20:28:48: 13000000 INFO @ Sat, 15 Jan 2022 20:28:49: 7000000 INFO @ Sat, 15 Jan 2022 20:28:50: 2000000 INFO @ Sat, 15 Jan 2022 20:28:54: 14000000 INFO @ Sat, 15 Jan 2022 20:28:55: 8000000 INFO @ Sat, 15 Jan 2022 20:28:55: 3000000 INFO @ Sat, 15 Jan 2022 20:28:59: 15000000 INFO @ Sat, 15 Jan 2022 20:29:01: 9000000 INFO @ Sat, 15 Jan 2022 20:29:01: 4000000 INFO @ Sat, 15 Jan 2022 20:29:05: 16000000 INFO @ Sat, 15 Jan 2022 20:29:06: 10000000 INFO @ Sat, 15 Jan 2022 20:29:07: 5000000 INFO @ Sat, 15 Jan 2022 20:29:11: 17000000 INFO @ Sat, 15 Jan 2022 20:29:12: 11000000 INFO @ Sat, 15 Jan 2022 20:29:12: 6000000 INFO @ Sat, 15 Jan 2022 20:29:16: 18000000 INFO @ Sat, 15 Jan 2022 20:29:18: 7000000 INFO @ Sat, 15 Jan 2022 20:29:18: 12000000 INFO @ Sat, 15 Jan 2022 20:29:22: 19000000 INFO @ Sat, 15 Jan 2022 20:29:23: 8000000 INFO @ Sat, 15 Jan 2022 20:29:24: 13000000 INFO @ Sat, 15 Jan 2022 20:29:27: 20000000 INFO @ Sat, 15 Jan 2022 20:29:29: 9000000 INFO @ Sat, 15 Jan 2022 20:29:29: 14000000 INFO @ Sat, 15 Jan 2022 20:29:33: 21000000 INFO @ Sat, 15 Jan 2022 20:29:35: 10000000 INFO @ Sat, 15 Jan 2022 20:29:35: 15000000 INFO @ Sat, 15 Jan 2022 20:29:38: 22000000 INFO @ Sat, 15 Jan 2022 20:29:40: 11000000 INFO @ Sat, 15 Jan 2022 20:29:41: 16000000 INFO @ Sat, 15 Jan 2022 20:29:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:29:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:29:43: #1 total tags in treatment: 7871546 INFO @ Sat, 15 Jan 2022 20:29:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:29:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:29:43: #1 tags after filtering in treatment: 6302628 INFO @ Sat, 15 Jan 2022 20:29:43: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:29:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:29:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:29:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:29:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:29:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:29:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:29:46: 12000000 INFO @ Sat, 15 Jan 2022 20:29:46: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:29:51: 13000000 INFO @ Sat, 15 Jan 2022 20:29:52: 18000000 INFO @ Sat, 15 Jan 2022 20:29:57: 14000000 INFO @ Sat, 15 Jan 2022 20:29:58: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:30:03: 15000000 INFO @ Sat, 15 Jan 2022 20:30:03: 20000000 INFO @ Sat, 15 Jan 2022 20:30:08: 16000000 INFO @ Sat, 15 Jan 2022 20:30:09: 21000000 INFO @ Sat, 15 Jan 2022 20:30:14: 17000000 INFO @ Sat, 15 Jan 2022 20:30:15: 22000000 INFO @ Sat, 15 Jan 2022 20:30:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:30:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:30:19: #1 total tags in treatment: 7871546 INFO @ Sat, 15 Jan 2022 20:30:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:19: #1 tags after filtering in treatment: 6302628 INFO @ Sat, 15 Jan 2022 20:30:19: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:30:19: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:30:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:30:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:20: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 20:30:20: 18000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:30:25: 19000000 INFO @ Sat, 15 Jan 2022 20:30:30: 20000000 INFO @ Sat, 15 Jan 2022 20:30:35: 21000000 INFO @ Sat, 15 Jan 2022 20:30:40: 22000000 INFO @ Sat, 15 Jan 2022 20:30:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:30:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:30:43: #1 total tags in treatment: 7871546 INFO @ Sat, 15 Jan 2022 20:30:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:44: #1 tags after filtering in treatment: 6302628 INFO @ Sat, 15 Jan 2022 20:30:44: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:30:44: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:30:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:30:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245446/SRX8245446.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling