Job ID = 14520932 SRX = SRX8245445 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16524069 spots for SRR11684656/SRR11684656.sra Written 16524069 spots for SRR11684656/SRR11684656.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:21 16524069 reads; of these: 16524069 (100.00%) were paired; of these: 8015255 (48.51%) aligned concordantly 0 times 7853550 (47.53%) aligned concordantly exactly 1 time 655264 (3.97%) aligned concordantly >1 times ---- 8015255 pairs aligned concordantly 0 times; of these: 409247 (5.11%) aligned discordantly 1 time ---- 7606008 pairs aligned 0 times concordantly or discordantly; of these: 15212016 mates make up the pairs; of these: 8301740 (54.57%) aligned 0 times 6224697 (40.92%) aligned exactly 1 time 685579 (4.51%) aligned >1 times 74.88% overall alignment rate Time searching: 00:09:21 Overall time: 00:09:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 233890 / 8916966 = 0.0262 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:29:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:29:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:29:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:29:59: 1000000 INFO @ Sat, 15 Jan 2022 20:30:03: 2000000 INFO @ Sat, 15 Jan 2022 20:30:08: 3000000 INFO @ Sat, 15 Jan 2022 20:30:13: 4000000 INFO @ Sat, 15 Jan 2022 20:30:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:30:23: 6000000 INFO @ Sat, 15 Jan 2022 20:30:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:30:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:30:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:30:28: 7000000 INFO @ Sat, 15 Jan 2022 20:30:29: 1000000 INFO @ Sat, 15 Jan 2022 20:30:33: 8000000 INFO @ Sat, 15 Jan 2022 20:30:34: 2000000 INFO @ Sat, 15 Jan 2022 20:30:38: 9000000 INFO @ Sat, 15 Jan 2022 20:30:39: 3000000 INFO @ Sat, 15 Jan 2022 20:30:43: 10000000 INFO @ Sat, 15 Jan 2022 20:30:44: 4000000 INFO @ Sat, 15 Jan 2022 20:30:48: 11000000 INFO @ Sat, 15 Jan 2022 20:30:49: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:30:53: 12000000 INFO @ Sat, 15 Jan 2022 20:30:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:30:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:30:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:30:54: 6000000 INFO @ Sat, 15 Jan 2022 20:30:58: 13000000 INFO @ Sat, 15 Jan 2022 20:30:59: 7000000 INFO @ Sat, 15 Jan 2022 20:30:59: 1000000 INFO @ Sat, 15 Jan 2022 20:31:03: 14000000 INFO @ Sat, 15 Jan 2022 20:31:04: 8000000 INFO @ Sat, 15 Jan 2022 20:31:04: 2000000 INFO @ Sat, 15 Jan 2022 20:31:08: 15000000 INFO @ Sat, 15 Jan 2022 20:31:09: 9000000 INFO @ Sat, 15 Jan 2022 20:31:09: 3000000 INFO @ Sat, 15 Jan 2022 20:31:13: 16000000 INFO @ Sat, 15 Jan 2022 20:31:14: 10000000 INFO @ Sat, 15 Jan 2022 20:31:14: 4000000 INFO @ Sat, 15 Jan 2022 20:31:18: 17000000 INFO @ Sat, 15 Jan 2022 20:31:19: 11000000 INFO @ Sat, 15 Jan 2022 20:31:19: 5000000 INFO @ Sat, 15 Jan 2022 20:31:23: 18000000 INFO @ Sat, 15 Jan 2022 20:31:24: 12000000 INFO @ Sat, 15 Jan 2022 20:31:24: 6000000 INFO @ Sat, 15 Jan 2022 20:31:28: 19000000 INFO @ Sat, 15 Jan 2022 20:31:29: 13000000 INFO @ Sat, 15 Jan 2022 20:31:29: 7000000 INFO @ Sat, 15 Jan 2022 20:31:33: 20000000 INFO @ Sat, 15 Jan 2022 20:31:34: 14000000 INFO @ Sat, 15 Jan 2022 20:31:34: 8000000 INFO @ Sat, 15 Jan 2022 20:31:38: 21000000 INFO @ Sat, 15 Jan 2022 20:31:39: 15000000 INFO @ Sat, 15 Jan 2022 20:31:39: 9000000 INFO @ Sat, 15 Jan 2022 20:31:43: 22000000 INFO @ Sat, 15 Jan 2022 20:31:44: 16000000 INFO @ Sat, 15 Jan 2022 20:31:45: 10000000 INFO @ Sat, 15 Jan 2022 20:31:48: 23000000 INFO @ Sat, 15 Jan 2022 20:31:49: 17000000 INFO @ Sat, 15 Jan 2022 20:31:50: 11000000 INFO @ Sat, 15 Jan 2022 20:31:53: 24000000 INFO @ Sat, 15 Jan 2022 20:31:54: 18000000 INFO @ Sat, 15 Jan 2022 20:31:55: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:31:55: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:31:55: #1 total tags in treatment: 8279420 INFO @ Sat, 15 Jan 2022 20:31:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:31:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:31:55: #1 tags after filtering in treatment: 6547720 INFO @ Sat, 15 Jan 2022 20:31:55: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 20:31:55: #1 finished! INFO @ Sat, 15 Jan 2022 20:31:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:31:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:31:55: 12000000 INFO @ Sat, 15 Jan 2022 20:31:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:31:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:31:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:31:59: 19000000 INFO @ Sat, 15 Jan 2022 20:32:00: 13000000 INFO @ Sat, 15 Jan 2022 20:32:04: 20000000 INFO @ Sat, 15 Jan 2022 20:32:05: 14000000 INFO @ Sat, 15 Jan 2022 20:32:09: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:32:10: 15000000 INFO @ Sat, 15 Jan 2022 20:32:14: 22000000 INFO @ Sat, 15 Jan 2022 20:32:15: 16000000 INFO @ Sat, 15 Jan 2022 20:32:19: 23000000 INFO @ Sat, 15 Jan 2022 20:32:20: 17000000 INFO @ Sat, 15 Jan 2022 20:32:24: 24000000 INFO @ Sat, 15 Jan 2022 20:32:25: 18000000 INFO @ Sat, 15 Jan 2022 20:32:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:26: #1 total tags in treatment: 8279420 INFO @ Sat, 15 Jan 2022 20:32:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:26: #1 tags after filtering in treatment: 6547720 INFO @ Sat, 15 Jan 2022 20:32:26: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 20:32:26: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:32:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:32:30: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:32:36: 20000000 INFO @ Sat, 15 Jan 2022 20:32:41: 21000000 INFO @ Sat, 15 Jan 2022 20:32:46: 22000000 INFO @ Sat, 15 Jan 2022 20:32:51: 23000000 INFO @ Sat, 15 Jan 2022 20:32:56: 24000000 INFO @ Sat, 15 Jan 2022 20:32:57: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:57: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:57: #1 total tags in treatment: 8279420 INFO @ Sat, 15 Jan 2022 20:32:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:57: #1 tags after filtering in treatment: 6547720 INFO @ Sat, 15 Jan 2022 20:32:57: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 20:32:57: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:32:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245445/SRX8245445.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling