Job ID = 14520931 SRX = SRX8245444 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17948897 spots for SRR11684655/SRR11684655.sra Written 17948897 spots for SRR11684655/SRR11684655.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:54 17948897 reads; of these: 17948897 (100.00%) were paired; of these: 9036336 (50.34%) aligned concordantly 0 times 8218873 (45.79%) aligned concordantly exactly 1 time 693688 (3.86%) aligned concordantly >1 times ---- 9036336 pairs aligned concordantly 0 times; of these: 379586 (4.20%) aligned discordantly 1 time ---- 8656750 pairs aligned 0 times concordantly or discordantly; of these: 17313500 mates make up the pairs; of these: 9514445 (54.95%) aligned 0 times 7033591 (40.62%) aligned exactly 1 time 765464 (4.42%) aligned >1 times 73.50% overall alignment rate Time searching: 00:10:54 Overall time: 00:10:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 221649 / 9290909 = 0.0239 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:32:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:32:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:32:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:32:44: 1000000 INFO @ Sat, 15 Jan 2022 20:32:49: 2000000 INFO @ Sat, 15 Jan 2022 20:32:55: 3000000 INFO @ Sat, 15 Jan 2022 20:33:02: 4000000 INFO @ Sat, 15 Jan 2022 20:33:07: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:13: 6000000 INFO @ Sat, 15 Jan 2022 20:33:15: 1000000 INFO @ Sat, 15 Jan 2022 20:33:20: 7000000 INFO @ Sat, 15 Jan 2022 20:33:21: 2000000 INFO @ Sat, 15 Jan 2022 20:33:26: 8000000 INFO @ Sat, 15 Jan 2022 20:33:28: 3000000 INFO @ Sat, 15 Jan 2022 20:33:33: 9000000 INFO @ Sat, 15 Jan 2022 20:33:34: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:39: 10000000 INFO @ Sat, 15 Jan 2022 20:33:40: 5000000 INFO @ Sat, 15 Jan 2022 20:33:45: 1000000 INFO @ Sat, 15 Jan 2022 20:33:45: 11000000 INFO @ Sat, 15 Jan 2022 20:33:46: 6000000 INFO @ Sat, 15 Jan 2022 20:33:51: 2000000 INFO @ Sat, 15 Jan 2022 20:33:52: 12000000 INFO @ Sat, 15 Jan 2022 20:33:52: 7000000 INFO @ Sat, 15 Jan 2022 20:33:57: 3000000 INFO @ Sat, 15 Jan 2022 20:33:57: 13000000 INFO @ Sat, 15 Jan 2022 20:33:58: 8000000 INFO @ Sat, 15 Jan 2022 20:34:03: 14000000 INFO @ Sat, 15 Jan 2022 20:34:04: 4000000 INFO @ Sat, 15 Jan 2022 20:34:04: 9000000 INFO @ Sat, 15 Jan 2022 20:34:09: 15000000 INFO @ Sat, 15 Jan 2022 20:34:09: 5000000 INFO @ Sat, 15 Jan 2022 20:34:10: 10000000 INFO @ Sat, 15 Jan 2022 20:34:15: 16000000 INFO @ Sat, 15 Jan 2022 20:34:16: 6000000 INFO @ Sat, 15 Jan 2022 20:34:16: 11000000 INFO @ Sat, 15 Jan 2022 20:34:21: 17000000 INFO @ Sat, 15 Jan 2022 20:34:22: 7000000 INFO @ Sat, 15 Jan 2022 20:34:22: 12000000 INFO @ Sat, 15 Jan 2022 20:34:28: 18000000 INFO @ Sat, 15 Jan 2022 20:34:28: 8000000 INFO @ Sat, 15 Jan 2022 20:34:28: 13000000 INFO @ Sat, 15 Jan 2022 20:34:33: 19000000 INFO @ Sat, 15 Jan 2022 20:34:35: 9000000 INFO @ Sat, 15 Jan 2022 20:34:35: 14000000 INFO @ Sat, 15 Jan 2022 20:34:39: 20000000 INFO @ Sat, 15 Jan 2022 20:34:41: 10000000 INFO @ Sat, 15 Jan 2022 20:34:41: 15000000 INFO @ Sat, 15 Jan 2022 20:34:46: 21000000 INFO @ Sat, 15 Jan 2022 20:34:47: 16000000 INFO @ Sat, 15 Jan 2022 20:34:47: 11000000 INFO @ Sat, 15 Jan 2022 20:34:52: 22000000 INFO @ Sat, 15 Jan 2022 20:34:53: 17000000 INFO @ Sat, 15 Jan 2022 20:34:54: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:34:58: 23000000 INFO @ Sat, 15 Jan 2022 20:34:59: 18000000 INFO @ Sat, 15 Jan 2022 20:35:00: 13000000 INFO @ Sat, 15 Jan 2022 20:35:04: 24000000 INFO @ Sat, 15 Jan 2022 20:35:05: 19000000 INFO @ Sat, 15 Jan 2022 20:35:07: 14000000 INFO @ Sat, 15 Jan 2022 20:35:10: 25000000 INFO @ Sat, 15 Jan 2022 20:35:12: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:35:13: 15000000 INFO @ Sat, 15 Jan 2022 20:35:16: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:35:16: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:35:16: #1 total tags in treatment: 8694263 INFO @ Sat, 15 Jan 2022 20:35:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:35:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:35:16: #1 tags after filtering in treatment: 6811283 INFO @ Sat, 15 Jan 2022 20:35:16: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 20:35:16: #1 finished! INFO @ Sat, 15 Jan 2022 20:35:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:35:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:35:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:35:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:35:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:35:18: 21000000 INFO @ Sat, 15 Jan 2022 20:35:19: 16000000 INFO @ Sat, 15 Jan 2022 20:35:24: 22000000 INFO @ Sat, 15 Jan 2022 20:35:25: 17000000 INFO @ Sat, 15 Jan 2022 20:35:30: 23000000 INFO @ Sat, 15 Jan 2022 20:35:31: 18000000 INFO @ Sat, 15 Jan 2022 20:35:36: 24000000 INFO @ Sat, 15 Jan 2022 20:35:38: 19000000 INFO @ Sat, 15 Jan 2022 20:35:42: 25000000 INFO @ Sat, 15 Jan 2022 20:35:45: 20000000 INFO @ Sat, 15 Jan 2022 20:35:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:35:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:35:48: #1 total tags in treatment: 8694263 INFO @ Sat, 15 Jan 2022 20:35:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:35:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:35:49: #1 tags after filtering in treatment: 6811283 INFO @ Sat, 15 Jan 2022 20:35:49: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 20:35:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:35:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:35:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:35:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:35:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:35:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:35:50: 21000000 INFO @ Sat, 15 Jan 2022 20:35:56: 22000000 INFO @ Sat, 15 Jan 2022 20:36:03: 23000000 INFO @ Sat, 15 Jan 2022 20:36:09: 24000000 INFO @ Sat, 15 Jan 2022 20:36:15: 25000000 INFO @ Sat, 15 Jan 2022 20:36:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:36:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:36:20: #1 total tags in treatment: 8694263 INFO @ Sat, 15 Jan 2022 20:36:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:36:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:36:21: #1 tags after filtering in treatment: 6811283 INFO @ Sat, 15 Jan 2022 20:36:21: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 20:36:21: #1 finished! INFO @ Sat, 15 Jan 2022 20:36:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:36:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:36:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:36:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:36:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245444/SRX8245444.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling