Job ID = 14520928 SRX = SRX8245441 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18375427 spots for SRR11684652/SRR11684652.sra Written 18375427 spots for SRR11684652/SRR11684652.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:43 18375427 reads; of these: 18375427 (100.00%) were paired; of these: 7986440 (43.46%) aligned concordantly 0 times 9568192 (52.07%) aligned concordantly exactly 1 time 820795 (4.47%) aligned concordantly >1 times ---- 7986440 pairs aligned concordantly 0 times; of these: 322828 (4.04%) aligned discordantly 1 time ---- 7663612 pairs aligned 0 times concordantly or discordantly; of these: 15327224 mates make up the pairs; of these: 9147723 (59.68%) aligned 0 times 5565851 (36.31%) aligned exactly 1 time 613650 (4.00%) aligned >1 times 75.11% overall alignment rate Time searching: 00:13:43 Overall time: 00:13:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 271271 / 10710387 = 0.0253 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:34:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:34:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:34:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:34:46: 1000000 INFO @ Sat, 15 Jan 2022 20:34:50: 2000000 INFO @ Sat, 15 Jan 2022 20:34:55: 3000000 INFO @ Sat, 15 Jan 2022 20:34:59: 4000000 INFO @ Sat, 15 Jan 2022 20:35:04: 5000000 INFO @ Sat, 15 Jan 2022 20:35:08: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:35:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:35:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:35:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:35:12: 7000000 INFO @ Sat, 15 Jan 2022 20:35:16: 1000000 INFO @ Sat, 15 Jan 2022 20:35:17: 8000000 INFO @ Sat, 15 Jan 2022 20:35:20: 2000000 INFO @ Sat, 15 Jan 2022 20:35:22: 9000000 INFO @ Sat, 15 Jan 2022 20:35:25: 3000000 INFO @ Sat, 15 Jan 2022 20:35:26: 10000000 INFO @ Sat, 15 Jan 2022 20:35:30: 4000000 INFO @ Sat, 15 Jan 2022 20:35:31: 11000000 INFO @ Sat, 15 Jan 2022 20:35:34: 5000000 INFO @ Sat, 15 Jan 2022 20:35:35: 12000000 INFO @ Sat, 15 Jan 2022 20:35:39: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:35:40: 13000000 INFO @ Sat, 15 Jan 2022 20:35:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:35:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:35:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:35:43: 7000000 INFO @ Sat, 15 Jan 2022 20:35:45: 14000000 INFO @ Sat, 15 Jan 2022 20:35:46: 1000000 INFO @ Sat, 15 Jan 2022 20:35:48: 8000000 INFO @ Sat, 15 Jan 2022 20:35:49: 15000000 INFO @ Sat, 15 Jan 2022 20:35:51: 2000000 INFO @ Sat, 15 Jan 2022 20:35:53: 9000000 INFO @ Sat, 15 Jan 2022 20:35:54: 16000000 INFO @ Sat, 15 Jan 2022 20:35:56: 3000000 INFO @ Sat, 15 Jan 2022 20:35:58: 10000000 INFO @ Sat, 15 Jan 2022 20:35:59: 17000000 INFO @ Sat, 15 Jan 2022 20:36:01: 4000000 INFO @ Sat, 15 Jan 2022 20:36:03: 11000000 INFO @ Sat, 15 Jan 2022 20:36:03: 18000000 INFO @ Sat, 15 Jan 2022 20:36:05: 5000000 INFO @ Sat, 15 Jan 2022 20:36:07: 12000000 INFO @ Sat, 15 Jan 2022 20:36:08: 19000000 INFO @ Sat, 15 Jan 2022 20:36:10: 6000000 INFO @ Sat, 15 Jan 2022 20:36:12: 13000000 INFO @ Sat, 15 Jan 2022 20:36:12: 20000000 INFO @ Sat, 15 Jan 2022 20:36:15: 7000000 INFO @ Sat, 15 Jan 2022 20:36:17: 14000000 INFO @ Sat, 15 Jan 2022 20:36:17: 21000000 INFO @ Sat, 15 Jan 2022 20:36:20: 8000000 INFO @ Sat, 15 Jan 2022 20:36:22: 22000000 INFO @ Sat, 15 Jan 2022 20:36:22: 15000000 INFO @ Sat, 15 Jan 2022 20:36:25: 9000000 INFO @ Sat, 15 Jan 2022 20:36:26: 23000000 INFO @ Sat, 15 Jan 2022 20:36:27: 16000000 INFO @ Sat, 15 Jan 2022 20:36:30: 10000000 INFO @ Sat, 15 Jan 2022 20:36:31: 24000000 INFO @ Sat, 15 Jan 2022 20:36:31: 17000000 INFO @ Sat, 15 Jan 2022 20:36:34: 11000000 INFO @ Sat, 15 Jan 2022 20:36:35: 25000000 INFO @ Sat, 15 Jan 2022 20:36:36: 18000000 INFO @ Sat, 15 Jan 2022 20:36:39: 12000000 INFO @ Sat, 15 Jan 2022 20:36:40: 26000000 INFO @ Sat, 15 Jan 2022 20:36:41: 19000000 INFO @ Sat, 15 Jan 2022 20:36:44: 13000000 INFO @ Sat, 15 Jan 2022 20:36:44: 27000000 INFO @ Sat, 15 Jan 2022 20:36:45: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:36:45: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:36:45: #1 total tags in treatment: 10120290 INFO @ Sat, 15 Jan 2022 20:36:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:36:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:36:45: #1 tags after filtering in treatment: 7691219 INFO @ Sat, 15 Jan 2022 20:36:45: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:36:45: #1 finished! INFO @ Sat, 15 Jan 2022 20:36:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:36:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:36:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:36:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:36:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:36:46: 20000000 INFO @ Sat, 15 Jan 2022 20:36:49: 14000000 INFO @ Sat, 15 Jan 2022 20:36:51: 21000000 INFO @ Sat, 15 Jan 2022 20:36:53: 15000000 INFO @ Sat, 15 Jan 2022 20:36:55: 22000000 INFO @ Sat, 15 Jan 2022 20:36:58: 16000000 INFO @ Sat, 15 Jan 2022 20:37:00: 23000000 INFO @ Sat, 15 Jan 2022 20:37:02: 17000000 INFO @ Sat, 15 Jan 2022 20:37:05: 24000000 INFO @ Sat, 15 Jan 2022 20:37:07: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:37:10: 25000000 INFO @ Sat, 15 Jan 2022 20:37:11: 19000000 INFO @ Sat, 15 Jan 2022 20:37:14: 26000000 INFO @ Sat, 15 Jan 2022 20:37:16: 20000000 INFO @ Sat, 15 Jan 2022 20:37:19: 27000000 INFO @ Sat, 15 Jan 2022 20:37:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:37:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:37:19: #1 total tags in treatment: 10120290 INFO @ Sat, 15 Jan 2022 20:37:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:37:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:37:19: #1 tags after filtering in treatment: 7691219 INFO @ Sat, 15 Jan 2022 20:37:19: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:37:19: #1 finished! INFO @ Sat, 15 Jan 2022 20:37:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:37:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:37:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:37:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:37:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:37:21: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:37:25: 22000000 INFO @ Sat, 15 Jan 2022 20:37:30: 23000000 INFO @ Sat, 15 Jan 2022 20:37:34: 24000000 INFO @ Sat, 15 Jan 2022 20:37:38: 25000000 INFO @ Sat, 15 Jan 2022 20:37:43: 26000000 INFO @ Sat, 15 Jan 2022 20:37:47: 27000000 INFO @ Sat, 15 Jan 2022 20:37:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:37:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:37:48: #1 total tags in treatment: 10120290 INFO @ Sat, 15 Jan 2022 20:37:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:37:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:37:48: #1 tags after filtering in treatment: 7691219 INFO @ Sat, 15 Jan 2022 20:37:48: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:37:48: #1 finished! INFO @ Sat, 15 Jan 2022 20:37:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:37:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:37:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:37:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:37:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245441/SRX8245441.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling