Job ID = 14520927 SRX = SRX8245440 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14499412 spots for SRR11684651/SRR11684651.sra Written 14499412 spots for SRR11684651/SRR11684651.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:04 14499412 reads; of these: 14499412 (100.00%) were paired; of these: 6310061 (43.52%) aligned concordantly 0 times 7547056 (52.05%) aligned concordantly exactly 1 time 642295 (4.43%) aligned concordantly >1 times ---- 6310061 pairs aligned concordantly 0 times; of these: 245786 (3.90%) aligned discordantly 1 time ---- 6064275 pairs aligned 0 times concordantly or discordantly; of these: 12128550 mates make up the pairs; of these: 7114390 (58.66%) aligned 0 times 4519225 (37.26%) aligned exactly 1 time 494935 (4.08%) aligned >1 times 75.47% overall alignment rate Time searching: 00:08:04 Overall time: 00:08:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 121329 / 8434006 = 0.0144 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:27: 1000000 INFO @ Sat, 15 Jan 2022 20:31:33: 2000000 INFO @ Sat, 15 Jan 2022 20:31:39: 3000000 INFO @ Sat, 15 Jan 2022 20:31:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:50: 5000000 INFO @ Sat, 15 Jan 2022 20:31:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:56: 6000000 INFO @ Sat, 15 Jan 2022 20:31:58: 1000000 INFO @ Sat, 15 Jan 2022 20:32:03: 7000000 INFO @ Sat, 15 Jan 2022 20:32:05: 2000000 INFO @ Sat, 15 Jan 2022 20:32:10: 8000000 INFO @ Sat, 15 Jan 2022 20:32:12: 3000000 INFO @ Sat, 15 Jan 2022 20:32:16: 9000000 INFO @ Sat, 15 Jan 2022 20:32:19: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:32:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:32:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:32:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:32:23: 10000000 INFO @ Sat, 15 Jan 2022 20:32:25: 5000000 INFO @ Sat, 15 Jan 2022 20:32:28: 1000000 INFO @ Sat, 15 Jan 2022 20:32:30: 11000000 INFO @ Sat, 15 Jan 2022 20:32:32: 6000000 INFO @ Sat, 15 Jan 2022 20:32:35: 2000000 INFO @ Sat, 15 Jan 2022 20:32:37: 12000000 INFO @ Sat, 15 Jan 2022 20:32:39: 7000000 INFO @ Sat, 15 Jan 2022 20:32:42: 3000000 INFO @ Sat, 15 Jan 2022 20:32:44: 13000000 INFO @ Sat, 15 Jan 2022 20:32:46: 8000000 INFO @ Sat, 15 Jan 2022 20:32:49: 4000000 INFO @ Sat, 15 Jan 2022 20:32:51: 14000000 INFO @ Sat, 15 Jan 2022 20:32:53: 9000000 INFO @ Sat, 15 Jan 2022 20:32:56: 5000000 INFO @ Sat, 15 Jan 2022 20:32:58: 15000000 INFO @ Sat, 15 Jan 2022 20:33:01: 10000000 INFO @ Sat, 15 Jan 2022 20:33:03: 6000000 INFO @ Sat, 15 Jan 2022 20:33:05: 16000000 INFO @ Sat, 15 Jan 2022 20:33:08: 11000000 INFO @ Sat, 15 Jan 2022 20:33:10: 7000000 INFO @ Sat, 15 Jan 2022 20:33:12: 17000000 INFO @ Sat, 15 Jan 2022 20:33:14: 12000000 INFO @ Sat, 15 Jan 2022 20:33:17: 8000000 INFO @ Sat, 15 Jan 2022 20:33:19: 18000000 INFO @ Sat, 15 Jan 2022 20:33:21: 13000000 INFO @ Sat, 15 Jan 2022 20:33:24: 9000000 INFO @ Sat, 15 Jan 2022 20:33:25: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:33:28: 14000000 INFO @ Sat, 15 Jan 2022 20:33:30: 10000000 INFO @ Sat, 15 Jan 2022 20:33:32: 20000000 INFO @ Sat, 15 Jan 2022 20:33:35: 15000000 INFO @ Sat, 15 Jan 2022 20:33:37: 11000000 INFO @ Sat, 15 Jan 2022 20:33:39: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:33:42: 16000000 INFO @ Sat, 15 Jan 2022 20:33:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:33:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:33:43: #1 total tags in treatment: 8069004 INFO @ Sat, 15 Jan 2022 20:33:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:33:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:33:43: #1 tags after filtering in treatment: 6451280 INFO @ Sat, 15 Jan 2022 20:33:43: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:33:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:33:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:33:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:33:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:33:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:33:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:33:44: 12000000 INFO @ Sat, 15 Jan 2022 20:33:48: 17000000 INFO @ Sat, 15 Jan 2022 20:33:51: 13000000 INFO @ Sat, 15 Jan 2022 20:33:55: 18000000 INFO @ Sat, 15 Jan 2022 20:33:58: 14000000 INFO @ Sat, 15 Jan 2022 20:34:01: 19000000 INFO @ Sat, 15 Jan 2022 20:34:04: 15000000 INFO @ Sat, 15 Jan 2022 20:34:08: 20000000 INFO @ Sat, 15 Jan 2022 20:34:11: 16000000 INFO @ Sat, 15 Jan 2022 20:34:14: 21000000 INFO @ Sat, 15 Jan 2022 20:34:17: 17000000 INFO @ Sat, 15 Jan 2022 20:34:18: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:34:18: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:34:18: #1 total tags in treatment: 8069004 INFO @ Sat, 15 Jan 2022 20:34:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:34:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:34:18: #1 tags after filtering in treatment: 6451280 INFO @ Sat, 15 Jan 2022 20:34:18: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:34:18: #1 finished! INFO @ Sat, 15 Jan 2022 20:34:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:34:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:34:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:34:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:34:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:34:23: 18000000 INFO @ Sat, 15 Jan 2022 20:34:29: 19000000 INFO @ Sat, 15 Jan 2022 20:34:34: 20000000 INFO @ Sat, 15 Jan 2022 20:34:40: 21000000 INFO @ Sat, 15 Jan 2022 20:34:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:34:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:34:43: #1 total tags in treatment: 8069004 INFO @ Sat, 15 Jan 2022 20:34:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:34:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:34:43: #1 tags after filtering in treatment: 6451280 INFO @ Sat, 15 Jan 2022 20:34:43: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:34:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:34:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:34:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:34:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:34:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:34:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245440/SRX8245440.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling