Job ID = 14520894 SRX = SRX8245430 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18482641 spots for SRR11684641/SRR11684641.sra Written 18482641 spots for SRR11684641/SRR11684641.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:01 18482641 reads; of these: 18482641 (100.00%) were paired; of these: 9011816 (48.76%) aligned concordantly 0 times 8475401 (45.86%) aligned concordantly exactly 1 time 995424 (5.39%) aligned concordantly >1 times ---- 9011816 pairs aligned concordantly 0 times; of these: 287113 (3.19%) aligned discordantly 1 time ---- 8724703 pairs aligned 0 times concordantly or discordantly; of these: 17449406 mates make up the pairs; of these: 10095754 (57.86%) aligned 0 times 6456357 (37.00%) aligned exactly 1 time 897295 (5.14%) aligned >1 times 72.69% overall alignment rate Time searching: 00:15:01 Overall time: 00:15:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 179398 / 9756793 = 0.0184 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:36:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:36:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:36:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:36:58: 1000000 INFO @ Sat, 15 Jan 2022 20:37:06: 2000000 INFO @ Sat, 15 Jan 2022 20:37:14: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:37:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:37:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:37:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:37:22: 4000000 INFO @ Sat, 15 Jan 2022 20:37:28: 1000000 INFO @ Sat, 15 Jan 2022 20:37:31: 5000000 INFO @ Sat, 15 Jan 2022 20:37:35: 2000000 INFO @ Sat, 15 Jan 2022 20:37:39: 6000000 INFO @ Sat, 15 Jan 2022 20:37:42: 3000000 INFO @ Sat, 15 Jan 2022 20:37:47: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:37:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:37:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:37:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:37:50: 4000000 INFO @ Sat, 15 Jan 2022 20:37:55: 8000000 INFO @ Sat, 15 Jan 2022 20:37:58: 5000000 INFO @ Sat, 15 Jan 2022 20:38:00: 1000000 INFO @ Sat, 15 Jan 2022 20:38:04: 9000000 INFO @ Sat, 15 Jan 2022 20:38:06: 6000000 INFO @ Sat, 15 Jan 2022 20:38:11: 2000000 INFO @ Sat, 15 Jan 2022 20:38:12: 10000000 INFO @ Sat, 15 Jan 2022 20:38:13: 7000000 INFO @ Sat, 15 Jan 2022 20:38:20: 11000000 INFO @ Sat, 15 Jan 2022 20:38:21: 8000000 INFO @ Sat, 15 Jan 2022 20:38:22: 3000000 INFO @ Sat, 15 Jan 2022 20:38:29: 12000000 INFO @ Sat, 15 Jan 2022 20:38:29: 9000000 INFO @ Sat, 15 Jan 2022 20:38:32: 4000000 INFO @ Sat, 15 Jan 2022 20:38:37: 10000000 INFO @ Sat, 15 Jan 2022 20:38:37: 13000000 INFO @ Sat, 15 Jan 2022 20:38:43: 5000000 INFO @ Sat, 15 Jan 2022 20:38:44: 11000000 INFO @ Sat, 15 Jan 2022 20:38:45: 14000000 INFO @ Sat, 15 Jan 2022 20:38:52: 12000000 INFO @ Sat, 15 Jan 2022 20:38:53: 6000000 INFO @ Sat, 15 Jan 2022 20:38:53: 15000000 INFO @ Sat, 15 Jan 2022 20:38:59: 13000000 INFO @ Sat, 15 Jan 2022 20:39:01: 16000000 INFO @ Sat, 15 Jan 2022 20:39:04: 7000000 INFO @ Sat, 15 Jan 2022 20:39:06: 14000000 INFO @ Sat, 15 Jan 2022 20:39:10: 17000000 INFO @ Sat, 15 Jan 2022 20:39:14: 15000000 INFO @ Sat, 15 Jan 2022 20:39:14: 8000000 INFO @ Sat, 15 Jan 2022 20:39:18: 18000000 INFO @ Sat, 15 Jan 2022 20:39:22: 16000000 INFO @ Sat, 15 Jan 2022 20:39:25: 9000000 INFO @ Sat, 15 Jan 2022 20:39:26: 19000000 INFO @ Sat, 15 Jan 2022 20:39:29: 17000000 INFO @ Sat, 15 Jan 2022 20:39:34: 20000000 INFO @ Sat, 15 Jan 2022 20:39:35: 10000000 INFO @ Sat, 15 Jan 2022 20:39:36: 18000000 INFO @ Sat, 15 Jan 2022 20:39:42: 21000000 INFO @ Sat, 15 Jan 2022 20:39:44: 19000000 INFO @ Sat, 15 Jan 2022 20:39:46: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:39:51: 22000000 INFO @ Sat, 15 Jan 2022 20:39:51: 20000000 INFO @ Sat, 15 Jan 2022 20:39:56: 12000000 INFO @ Sat, 15 Jan 2022 20:39:59: 21000000 INFO @ Sat, 15 Jan 2022 20:39:59: 23000000 INFO @ Sat, 15 Jan 2022 20:40:06: 22000000 INFO @ Sat, 15 Jan 2022 20:40:07: 13000000 INFO @ Sat, 15 Jan 2022 20:40:07: 24000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:40:14: 23000000 INFO @ Sat, 15 Jan 2022 20:40:15: 25000000 INFO @ Sat, 15 Jan 2022 20:40:17: 14000000 INFO @ Sat, 15 Jan 2022 20:40:21: 24000000 INFO @ Sat, 15 Jan 2022 20:40:24: 26000000 INFO @ Sat, 15 Jan 2022 20:40:28: 15000000 INFO @ Sat, 15 Jan 2022 20:40:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:40:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:40:28: #1 total tags in treatment: 9292503 INFO @ Sat, 15 Jan 2022 20:40:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:40:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:40:28: #1 tags after filtering in treatment: 7023508 INFO @ Sat, 15 Jan 2022 20:40:28: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:40:28: #1 finished! INFO @ Sat, 15 Jan 2022 20:40:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:40:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:40:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:40:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:40:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:40:29: 25000000 INFO @ Sat, 15 Jan 2022 20:40:37: 26000000 INFO @ Sat, 15 Jan 2022 20:40:38: 16000000 INFO @ Sat, 15 Jan 2022 20:40:41: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:40:41: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:40:41: #1 total tags in treatment: 9292503 INFO @ Sat, 15 Jan 2022 20:40:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:40:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:40:41: #1 tags after filtering in treatment: 7023508 INFO @ Sat, 15 Jan 2022 20:40:41: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:40:41: #1 finished! INFO @ Sat, 15 Jan 2022 20:40:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:40:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:40:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:40:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:40:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:40:48: 17000000 INFO @ Sat, 15 Jan 2022 20:40:58: 18000000 INFO @ Sat, 15 Jan 2022 20:41:08: 19000000 INFO @ Sat, 15 Jan 2022 20:41:19: 20000000 INFO @ Sat, 15 Jan 2022 20:41:29: 21000000 INFO @ Sat, 15 Jan 2022 20:41:39: 22000000 INFO @ Sat, 15 Jan 2022 20:41:49: 23000000 INFO @ Sat, 15 Jan 2022 20:41:59: 24000000 INFO @ Sat, 15 Jan 2022 20:42:08: 25000000 INFO @ Sat, 15 Jan 2022 20:42:18: 26000000 INFO @ Sat, 15 Jan 2022 20:42:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:42:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:42:22: #1 total tags in treatment: 9292503 INFO @ Sat, 15 Jan 2022 20:42:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:42:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:42:23: #1 tags after filtering in treatment: 7023508 INFO @ Sat, 15 Jan 2022 20:42:23: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:42:23: #1 finished! INFO @ Sat, 15 Jan 2022 20:42:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:42:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:42:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:42:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:42:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245430/SRX8245430.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling