Job ID = 14520859 SRX = SRX8245428 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19603753 spots for SRR11684639/SRR11684639.sra Written 19603753 spots for SRR11684639/SRR11684639.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:45 19603753 reads; of these: 19603753 (100.00%) were paired; of these: 9412838 (48.02%) aligned concordantly 0 times 9141399 (46.63%) aligned concordantly exactly 1 time 1049516 (5.35%) aligned concordantly >1 times ---- 9412838 pairs aligned concordantly 0 times; of these: 371873 (3.95%) aligned discordantly 1 time ---- 9040965 pairs aligned 0 times concordantly or discordantly; of these: 18081930 mates make up the pairs; of these: 10405520 (57.55%) aligned 0 times 6730505 (37.22%) aligned exactly 1 time 945905 (5.23%) aligned >1 times 73.46% overall alignment rate Time searching: 00:17:45 Overall time: 00:17:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 290585 / 10561553 = 0.0275 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:29:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:29:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:29:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:29:58: 1000000 INFO @ Sat, 15 Jan 2022 20:30:03: 2000000 INFO @ Sat, 15 Jan 2022 20:30:07: 3000000 INFO @ Sat, 15 Jan 2022 20:30:12: 4000000 INFO @ Sat, 15 Jan 2022 20:30:16: 5000000 INFO @ Sat, 15 Jan 2022 20:30:21: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:30:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:30:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:30:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:30:26: 7000000 INFO @ Sat, 15 Jan 2022 20:30:29: 1000000 INFO @ Sat, 15 Jan 2022 20:30:30: 8000000 INFO @ Sat, 15 Jan 2022 20:30:34: 2000000 INFO @ Sat, 15 Jan 2022 20:30:35: 9000000 INFO @ Sat, 15 Jan 2022 20:30:40: 3000000 INFO @ Sat, 15 Jan 2022 20:30:40: 10000000 INFO @ Sat, 15 Jan 2022 20:30:45: 11000000 INFO @ Sat, 15 Jan 2022 20:30:45: 4000000 INFO @ Sat, 15 Jan 2022 20:30:50: 12000000 INFO @ Sat, 15 Jan 2022 20:30:51: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:30:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:30:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:30:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:30:55: 13000000 INFO @ Sat, 15 Jan 2022 20:30:56: 6000000 INFO @ Sat, 15 Jan 2022 20:30:59: 1000000 INFO @ Sat, 15 Jan 2022 20:30:59: 14000000 INFO @ Sat, 15 Jan 2022 20:31:02: 7000000 INFO @ Sat, 15 Jan 2022 20:31:04: 15000000 INFO @ Sat, 15 Jan 2022 20:31:05: 2000000 INFO @ Sat, 15 Jan 2022 20:31:07: 8000000 INFO @ Sat, 15 Jan 2022 20:31:09: 16000000 INFO @ Sat, 15 Jan 2022 20:31:11: 3000000 INFO @ Sat, 15 Jan 2022 20:31:13: 9000000 INFO @ Sat, 15 Jan 2022 20:31:14: 17000000 INFO @ Sat, 15 Jan 2022 20:31:17: 4000000 INFO @ Sat, 15 Jan 2022 20:31:18: 10000000 INFO @ Sat, 15 Jan 2022 20:31:19: 18000000 INFO @ Sat, 15 Jan 2022 20:31:22: 5000000 INFO @ Sat, 15 Jan 2022 20:31:24: 19000000 INFO @ Sat, 15 Jan 2022 20:31:24: 11000000 INFO @ Sat, 15 Jan 2022 20:31:28: 6000000 INFO @ Sat, 15 Jan 2022 20:31:29: 20000000 INFO @ Sat, 15 Jan 2022 20:31:30: 12000000 INFO @ Sat, 15 Jan 2022 20:31:34: 21000000 INFO @ Sat, 15 Jan 2022 20:31:34: 7000000 INFO @ Sat, 15 Jan 2022 20:31:35: 13000000 INFO @ Sat, 15 Jan 2022 20:31:38: 22000000 INFO @ Sat, 15 Jan 2022 20:31:40: 8000000 INFO @ Sat, 15 Jan 2022 20:31:41: 14000000 INFO @ Sat, 15 Jan 2022 20:31:43: 23000000 INFO @ Sat, 15 Jan 2022 20:31:45: 9000000 INFO @ Sat, 15 Jan 2022 20:31:46: 15000000 INFO @ Sat, 15 Jan 2022 20:31:48: 24000000 INFO @ Sat, 15 Jan 2022 20:31:51: 10000000 INFO @ Sat, 15 Jan 2022 20:31:52: 16000000 INFO @ Sat, 15 Jan 2022 20:31:53: 25000000 INFO @ Sat, 15 Jan 2022 20:31:57: 11000000 INFO @ Sat, 15 Jan 2022 20:31:57: 17000000 INFO @ Sat, 15 Jan 2022 20:31:58: 26000000 INFO @ Sat, 15 Jan 2022 20:32:03: 12000000 INFO @ Sat, 15 Jan 2022 20:32:03: 27000000 INFO @ Sat, 15 Jan 2022 20:32:03: 18000000 INFO @ Sat, 15 Jan 2022 20:32:08: 28000000 INFO @ Sat, 15 Jan 2022 20:32:08: 13000000 INFO @ Sat, 15 Jan 2022 20:32:08: 19000000 INFO @ Sat, 15 Jan 2022 20:32:09: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:09: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:09: #1 total tags in treatment: 9903088 INFO @ Sat, 15 Jan 2022 20:32:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:09: #1 tags after filtering in treatment: 7389858 INFO @ Sat, 15 Jan 2022 20:32:09: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:32:09: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:32:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 252 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:32:14: 14000000 INFO @ Sat, 15 Jan 2022 20:32:14: 20000000 INFO @ Sat, 15 Jan 2022 20:32:19: 15000000 INFO @ Sat, 15 Jan 2022 20:32:19: 21000000 INFO @ Sat, 15 Jan 2022 20:32:25: 16000000 INFO @ Sat, 15 Jan 2022 20:32:25: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:32:30: 17000000 INFO @ Sat, 15 Jan 2022 20:32:30: 23000000 INFO @ Sat, 15 Jan 2022 20:32:35: 18000000 INFO @ Sat, 15 Jan 2022 20:32:36: 24000000 INFO @ Sat, 15 Jan 2022 20:32:41: 19000000 INFO @ Sat, 15 Jan 2022 20:32:42: 25000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:32:47: 20000000 INFO @ Sat, 15 Jan 2022 20:32:47: 26000000 INFO @ Sat, 15 Jan 2022 20:32:53: 27000000 INFO @ Sat, 15 Jan 2022 20:32:53: 21000000 INFO @ Sat, 15 Jan 2022 20:32:58: 28000000 INFO @ Sat, 15 Jan 2022 20:32:59: 22000000 INFO @ Sat, 15 Jan 2022 20:32:59: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:59: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:59: #1 total tags in treatment: 9903088 INFO @ Sat, 15 Jan 2022 20:32:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:33:00: #1 tags after filtering in treatment: 7389858 INFO @ Sat, 15 Jan 2022 20:33:00: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:33:00: #1 finished! INFO @ Sat, 15 Jan 2022 20:33:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:33:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:33:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:33:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:33:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:33:04: 23000000 INFO @ Sat, 15 Jan 2022 20:33:10: 24000000 INFO @ Sat, 15 Jan 2022 20:33:16: 25000000 INFO @ Sat, 15 Jan 2022 20:33:21: 26000000 INFO @ Sat, 15 Jan 2022 20:33:27: 27000000 INFO @ Sat, 15 Jan 2022 20:33:32: 28000000 INFO @ Sat, 15 Jan 2022 20:33:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:33:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:33:34: #1 total tags in treatment: 9903088 INFO @ Sat, 15 Jan 2022 20:33:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:33:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:33:34: #1 tags after filtering in treatment: 7389858 INFO @ Sat, 15 Jan 2022 20:33:34: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:33:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:33:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:33:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:33:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:33:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:33:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245428/SRX8245428.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling