Job ID = 14520858 SRX = SRX8245427 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 23691948 spots for SRR11684638/SRR11684638.sra Written 23691948 spots for SRR11684638/SRR11684638.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:42 23691948 reads; of these: 23691948 (100.00%) were paired; of these: 9913332 (41.84%) aligned concordantly 0 times 12102358 (51.08%) aligned concordantly exactly 1 time 1676258 (7.08%) aligned concordantly >1 times ---- 9913332 pairs aligned concordantly 0 times; of these: 344365 (3.47%) aligned discordantly 1 time ---- 9568967 pairs aligned 0 times concordantly or discordantly; of these: 19137934 mates make up the pairs; of these: 11372441 (59.42%) aligned 0 times 6676411 (34.89%) aligned exactly 1 time 1089082 (5.69%) aligned >1 times 76.00% overall alignment rate Time searching: 00:13:42 Overall time: 00:13:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 495486 / 14121506 = 0.0351 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:27:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:27:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:27:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:27:51: 1000000 INFO @ Sat, 15 Jan 2022 20:27:58: 2000000 INFO @ Sat, 15 Jan 2022 20:28:05: 3000000 INFO @ Sat, 15 Jan 2022 20:28:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:19: 5000000 INFO @ Sat, 15 Jan 2022 20:28:22: 1000000 INFO @ Sat, 15 Jan 2022 20:28:26: 6000000 INFO @ Sat, 15 Jan 2022 20:28:30: 2000000 INFO @ Sat, 15 Jan 2022 20:28:34: 7000000 INFO @ Sat, 15 Jan 2022 20:28:38: 3000000 INFO @ Sat, 15 Jan 2022 20:28:41: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:46: 4000000 INFO @ Sat, 15 Jan 2022 20:28:49: 9000000 INFO @ Sat, 15 Jan 2022 20:28:52: 1000000 INFO @ Sat, 15 Jan 2022 20:28:54: 5000000 INFO @ Sat, 15 Jan 2022 20:28:56: 10000000 INFO @ Sat, 15 Jan 2022 20:28:59: 2000000 INFO @ Sat, 15 Jan 2022 20:29:02: 6000000 INFO @ Sat, 15 Jan 2022 20:29:04: 11000000 INFO @ Sat, 15 Jan 2022 20:29:07: 3000000 INFO @ Sat, 15 Jan 2022 20:29:10: 7000000 INFO @ Sat, 15 Jan 2022 20:29:12: 12000000 INFO @ Sat, 15 Jan 2022 20:29:14: 4000000 INFO @ Sat, 15 Jan 2022 20:29:18: 8000000 INFO @ Sat, 15 Jan 2022 20:29:19: 13000000 INFO @ Sat, 15 Jan 2022 20:29:21: 5000000 INFO @ Sat, 15 Jan 2022 20:29:26: 9000000 INFO @ Sat, 15 Jan 2022 20:29:27: 14000000 INFO @ Sat, 15 Jan 2022 20:29:28: 6000000 INFO @ Sat, 15 Jan 2022 20:29:34: 10000000 INFO @ Sat, 15 Jan 2022 20:29:34: 15000000 INFO @ Sat, 15 Jan 2022 20:29:35: 7000000 INFO @ Sat, 15 Jan 2022 20:29:42: 16000000 INFO @ Sat, 15 Jan 2022 20:29:42: 11000000 INFO @ Sat, 15 Jan 2022 20:29:43: 8000000 INFO @ Sat, 15 Jan 2022 20:29:49: 17000000 INFO @ Sat, 15 Jan 2022 20:29:50: 12000000 INFO @ Sat, 15 Jan 2022 20:29:50: 9000000 INFO @ Sat, 15 Jan 2022 20:29:57: 18000000 INFO @ Sat, 15 Jan 2022 20:29:57: 10000000 INFO @ Sat, 15 Jan 2022 20:29:58: 13000000 INFO @ Sat, 15 Jan 2022 20:30:04: 11000000 INFO @ Sat, 15 Jan 2022 20:30:05: 19000000 INFO @ Sat, 15 Jan 2022 20:30:06: 14000000 INFO @ Sat, 15 Jan 2022 20:30:12: 12000000 INFO @ Sat, 15 Jan 2022 20:30:13: 20000000 INFO @ Sat, 15 Jan 2022 20:30:14: 15000000 INFO @ Sat, 15 Jan 2022 20:30:19: 13000000 INFO @ Sat, 15 Jan 2022 20:30:20: 21000000 INFO @ Sat, 15 Jan 2022 20:30:22: 16000000 INFO @ Sat, 15 Jan 2022 20:30:26: 14000000 INFO @ Sat, 15 Jan 2022 20:30:28: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:30:30: 17000000 INFO @ Sat, 15 Jan 2022 20:30:33: 15000000 INFO @ Sat, 15 Jan 2022 20:30:36: 23000000 INFO @ Sat, 15 Jan 2022 20:30:38: 18000000 INFO @ Sat, 15 Jan 2022 20:30:40: 16000000 INFO @ Sat, 15 Jan 2022 20:30:44: 24000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:30:46: 19000000 INFO @ Sat, 15 Jan 2022 20:30:47: 17000000 INFO @ Sat, 15 Jan 2022 20:30:52: 25000000 INFO @ Sat, 15 Jan 2022 20:30:54: 20000000 INFO @ Sat, 15 Jan 2022 20:30:54: 18000000 INFO @ Sat, 15 Jan 2022 20:31:01: 26000000 INFO @ Sat, 15 Jan 2022 20:31:02: 19000000 INFO @ Sat, 15 Jan 2022 20:31:02: 21000000 INFO @ Sat, 15 Jan 2022 20:31:09: 27000000 INFO @ Sat, 15 Jan 2022 20:31:09: 20000000 INFO @ Sat, 15 Jan 2022 20:31:11: 22000000 INFO @ Sat, 15 Jan 2022 20:31:16: 21000000 INFO @ Sat, 15 Jan 2022 20:31:17: 28000000 INFO @ Sat, 15 Jan 2022 20:31:19: 23000000 INFO @ Sat, 15 Jan 2022 20:31:23: 22000000 INFO @ Sat, 15 Jan 2022 20:31:25: 29000000 INFO @ Sat, 15 Jan 2022 20:31:27: 24000000 INFO @ Sat, 15 Jan 2022 20:31:30: 23000000 INFO @ Sat, 15 Jan 2022 20:31:33: 30000000 INFO @ Sat, 15 Jan 2022 20:31:35: 25000000 INFO @ Sat, 15 Jan 2022 20:31:37: 24000000 INFO @ Sat, 15 Jan 2022 20:31:41: 31000000 INFO @ Sat, 15 Jan 2022 20:31:43: 26000000 INFO @ Sat, 15 Jan 2022 20:31:45: 25000000 INFO @ Sat, 15 Jan 2022 20:31:49: 32000000 INFO @ Sat, 15 Jan 2022 20:31:52: 27000000 INFO @ Sat, 15 Jan 2022 20:31:52: 26000000 INFO @ Sat, 15 Jan 2022 20:31:57: 33000000 INFO @ Sat, 15 Jan 2022 20:31:59: 27000000 INFO @ Sat, 15 Jan 2022 20:32:00: 28000000 INFO @ Sat, 15 Jan 2022 20:32:06: 34000000 INFO @ Sat, 15 Jan 2022 20:32:06: 28000000 INFO @ Sat, 15 Jan 2022 20:32:07: 29000000 INFO @ Sat, 15 Jan 2022 20:32:13: 29000000 INFO @ Sat, 15 Jan 2022 20:32:14: 35000000 INFO @ Sat, 15 Jan 2022 20:32:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:14: #1 total tags in treatment: 13286102 INFO @ Sat, 15 Jan 2022 20:32:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:14: #1 tags after filtering in treatment: 9157023 INFO @ Sat, 15 Jan 2022 20:32:14: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 20:32:14: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:32:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:32:15: 30000000 INFO @ Sat, 15 Jan 2022 20:32:20: 30000000 INFO @ Sat, 15 Jan 2022 20:32:23: 31000000 INFO @ Sat, 15 Jan 2022 20:32:27: 31000000 INFO @ Sat, 15 Jan 2022 20:32:31: 32000000 INFO @ Sat, 15 Jan 2022 20:32:34: 32000000 INFO @ Sat, 15 Jan 2022 20:32:39: 33000000 INFO @ Sat, 15 Jan 2022 20:32:41: 33000000 INFO @ Sat, 15 Jan 2022 20:32:47: 34000000 INFO @ Sat, 15 Jan 2022 20:32:48: 34000000 INFO @ Sat, 15 Jan 2022 20:32:55: 35000000 INFO @ Sat, 15 Jan 2022 20:32:55: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:55: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:55: #1 total tags in treatment: 13286102 INFO @ Sat, 15 Jan 2022 20:32:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:55: #1 tags after filtering in treatment: 9157023 INFO @ Sat, 15 Jan 2022 20:32:55: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 20:32:55: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:56: 35000000 INFO @ Sat, 15 Jan 2022 20:32:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:56: #1 total tags in treatment: 13286102 INFO @ Sat, 15 Jan 2022 20:32:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:32:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:32:56: #1 tags after filtering in treatment: 9157023 INFO @ Sat, 15 Jan 2022 20:32:56: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 20:32:56: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:32:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245427/SRX8245427.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling