Job ID = 14520857 SRX = SRX8245426 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15018781 spots for SRR11684637/SRR11684637.sra Written 15018781 spots for SRR11684637/SRR11684637.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:21 15018781 reads; of these: 15018781 (100.00%) were paired; of these: 6320312 (42.08%) aligned concordantly 0 times 7727552 (51.45%) aligned concordantly exactly 1 time 970917 (6.46%) aligned concordantly >1 times ---- 6320312 pairs aligned concordantly 0 times; of these: 235787 (3.73%) aligned discordantly 1 time ---- 6084525 pairs aligned 0 times concordantly or discordantly; of these: 12169050 mates make up the pairs; of these: 7128674 (58.58%) aligned 0 times 4376289 (35.96%) aligned exactly 1 time 664087 (5.46%) aligned >1 times 76.27% overall alignment rate Time searching: 00:12:21 Overall time: 00:12:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 174293 / 8933224 = 0.0195 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:23:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:23:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:23:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:23:52: 1000000 INFO @ Sat, 15 Jan 2022 20:23:58: 2000000 INFO @ Sat, 15 Jan 2022 20:24:04: 3000000 INFO @ Sat, 15 Jan 2022 20:24:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:24:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:24:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:24:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:16: 5000000 INFO @ Sat, 15 Jan 2022 20:24:23: 1000000 INFO @ Sat, 15 Jan 2022 20:24:23: 6000000 INFO @ Sat, 15 Jan 2022 20:24:30: 2000000 INFO @ Sat, 15 Jan 2022 20:24:30: 7000000 INFO @ Sat, 15 Jan 2022 20:24:36: 3000000 INFO @ Sat, 15 Jan 2022 20:24:37: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:24:43: 4000000 INFO @ Sat, 15 Jan 2022 20:24:43: 9000000 INFO @ Sat, 15 Jan 2022 20:24:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:24:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:24:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:50: 5000000 INFO @ Sat, 15 Jan 2022 20:24:50: 10000000 INFO @ Sat, 15 Jan 2022 20:24:53: 1000000 INFO @ Sat, 15 Jan 2022 20:24:57: 6000000 INFO @ Sat, 15 Jan 2022 20:24:58: 11000000 INFO @ Sat, 15 Jan 2022 20:25:01: 2000000 INFO @ Sat, 15 Jan 2022 20:25:05: 7000000 INFO @ Sat, 15 Jan 2022 20:25:05: 12000000 INFO @ Sat, 15 Jan 2022 20:25:08: 3000000 INFO @ Sat, 15 Jan 2022 20:25:12: 13000000 INFO @ Sat, 15 Jan 2022 20:25:12: 8000000 INFO @ Sat, 15 Jan 2022 20:25:15: 4000000 INFO @ Sat, 15 Jan 2022 20:25:19: 14000000 INFO @ Sat, 15 Jan 2022 20:25:19: 9000000 INFO @ Sat, 15 Jan 2022 20:25:23: 5000000 INFO @ Sat, 15 Jan 2022 20:25:26: 15000000 INFO @ Sat, 15 Jan 2022 20:25:27: 10000000 INFO @ Sat, 15 Jan 2022 20:25:30: 6000000 INFO @ Sat, 15 Jan 2022 20:25:34: 16000000 INFO @ Sat, 15 Jan 2022 20:25:34: 11000000 INFO @ Sat, 15 Jan 2022 20:25:38: 7000000 INFO @ Sat, 15 Jan 2022 20:25:42: 17000000 INFO @ Sat, 15 Jan 2022 20:25:42: 12000000 INFO @ Sat, 15 Jan 2022 20:25:45: 8000000 INFO @ Sat, 15 Jan 2022 20:25:49: 18000000 INFO @ Sat, 15 Jan 2022 20:25:49: 13000000 INFO @ Sat, 15 Jan 2022 20:25:53: 9000000 INFO @ Sat, 15 Jan 2022 20:25:57: 14000000 INFO @ Sat, 15 Jan 2022 20:25:57: 19000000 INFO @ Sat, 15 Jan 2022 20:26:01: 10000000 INFO @ Sat, 15 Jan 2022 20:26:05: 15000000 INFO @ Sat, 15 Jan 2022 20:26:05: 20000000 INFO @ Sat, 15 Jan 2022 20:26:09: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:26:13: 21000000 INFO @ Sat, 15 Jan 2022 20:26:13: 16000000 INFO @ Sat, 15 Jan 2022 20:26:16: 12000000 INFO @ Sat, 15 Jan 2022 20:26:20: 22000000 INFO @ Sat, 15 Jan 2022 20:26:21: 17000000 INFO @ Sat, 15 Jan 2022 20:26:24: 13000000 INFO @ Sat, 15 Jan 2022 20:26:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:26:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:26:25: #1 total tags in treatment: 8525232 INFO @ Sat, 15 Jan 2022 20:26:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:25: #1 tags after filtering in treatment: 6550577 INFO @ Sat, 15 Jan 2022 20:26:25: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:26:25: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:26:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:26:29: 18000000 INFO @ Sat, 15 Jan 2022 20:26:32: 14000000 INFO @ Sat, 15 Jan 2022 20:26:37: 19000000 INFO @ Sat, 15 Jan 2022 20:26:40: 15000000 INFO @ Sat, 15 Jan 2022 20:26:44: 20000000 INFO @ Sat, 15 Jan 2022 20:26:48: 16000000 INFO @ Sat, 15 Jan 2022 20:26:52: 21000000 INFO @ Sat, 15 Jan 2022 20:26:56: 17000000 INFO @ Sat, 15 Jan 2022 20:27:00: 22000000 INFO @ Sat, 15 Jan 2022 20:27:04: 18000000 INFO @ Sat, 15 Jan 2022 20:27:05: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:27:05: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:27:05: #1 total tags in treatment: 8525232 INFO @ Sat, 15 Jan 2022 20:27:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:27:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:27:05: #1 tags after filtering in treatment: 6550577 INFO @ Sat, 15 Jan 2022 20:27:05: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:27:05: #1 finished! INFO @ Sat, 15 Jan 2022 20:27:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:27:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:27:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:27:12: 19000000 INFO @ Sat, 15 Jan 2022 20:27:19: 20000000 INFO @ Sat, 15 Jan 2022 20:27:27: 21000000 INFO @ Sat, 15 Jan 2022 20:27:35: 22000000 INFO @ Sat, 15 Jan 2022 20:27:39: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:27:39: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:27:39: #1 total tags in treatment: 8525232 INFO @ Sat, 15 Jan 2022 20:27:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:27:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:27:39: #1 tags after filtering in treatment: 6550577 INFO @ Sat, 15 Jan 2022 20:27:39: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:27:39: #1 finished! INFO @ Sat, 15 Jan 2022 20:27:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:27:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:27:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245426/SRX8245426.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling