Job ID = 14520856 SRX = SRX8245425 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17195443 spots for SRR11684636/SRR11684636.sra Written 17195443 spots for SRR11684636/SRR11684636.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:43 17195443 reads; of these: 17195443 (100.00%) were paired; of these: 7452044 (43.34%) aligned concordantly 0 times 8666075 (50.40%) aligned concordantly exactly 1 time 1077324 (6.27%) aligned concordantly >1 times ---- 7452044 pairs aligned concordantly 0 times; of these: 305204 (4.10%) aligned discordantly 1 time ---- 7146840 pairs aligned 0 times concordantly or discordantly; of these: 14293680 mates make up the pairs; of these: 8470421 (59.26%) aligned 0 times 5057149 (35.38%) aligned exactly 1 time 766110 (5.36%) aligned >1 times 75.37% overall alignment rate Time searching: 00:10:43 Overall time: 00:10:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 255939 / 10046962 = 0.0255 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:23:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:23:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:23:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:23:56: 1000000 INFO @ Sat, 15 Jan 2022 20:24:01: 2000000 INFO @ Sat, 15 Jan 2022 20:24:07: 3000000 INFO @ Sat, 15 Jan 2022 20:24:12: 4000000 INFO @ Sat, 15 Jan 2022 20:24:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:24:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:24:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:24:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:24: 6000000 INFO @ Sat, 15 Jan 2022 20:24:27: 1000000 INFO @ Sat, 15 Jan 2022 20:24:30: 7000000 INFO @ Sat, 15 Jan 2022 20:24:33: 2000000 INFO @ Sat, 15 Jan 2022 20:24:37: 8000000 INFO @ Sat, 15 Jan 2022 20:24:40: 3000000 INFO @ Sat, 15 Jan 2022 20:24:43: 9000000 INFO @ Sat, 15 Jan 2022 20:24:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:24:50: 10000000 INFO @ Sat, 15 Jan 2022 20:24:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:24:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:24:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:53: 5000000 INFO @ Sat, 15 Jan 2022 20:24:56: 11000000 INFO @ Sat, 15 Jan 2022 20:24:57: 1000000 INFO @ Sat, 15 Jan 2022 20:25:00: 6000000 INFO @ Sat, 15 Jan 2022 20:25:03: 12000000 INFO @ Sat, 15 Jan 2022 20:25:03: 2000000 INFO @ Sat, 15 Jan 2022 20:25:06: 7000000 INFO @ Sat, 15 Jan 2022 20:25:10: 13000000 INFO @ Sat, 15 Jan 2022 20:25:10: 3000000 INFO @ Sat, 15 Jan 2022 20:25:13: 8000000 INFO @ Sat, 15 Jan 2022 20:25:16: 14000000 INFO @ Sat, 15 Jan 2022 20:25:17: 4000000 INFO @ Sat, 15 Jan 2022 20:25:20: 9000000 INFO @ Sat, 15 Jan 2022 20:25:23: 15000000 INFO @ Sat, 15 Jan 2022 20:25:23: 5000000 INFO @ Sat, 15 Jan 2022 20:25:27: 10000000 INFO @ Sat, 15 Jan 2022 20:25:29: 16000000 INFO @ Sat, 15 Jan 2022 20:25:30: 6000000 INFO @ Sat, 15 Jan 2022 20:25:34: 11000000 INFO @ Sat, 15 Jan 2022 20:25:36: 17000000 INFO @ Sat, 15 Jan 2022 20:25:37: 7000000 INFO @ Sat, 15 Jan 2022 20:25:41: 12000000 INFO @ Sat, 15 Jan 2022 20:25:43: 18000000 INFO @ Sat, 15 Jan 2022 20:25:44: 8000000 INFO @ Sat, 15 Jan 2022 20:25:48: 13000000 INFO @ Sat, 15 Jan 2022 20:25:51: 19000000 INFO @ Sat, 15 Jan 2022 20:25:51: 9000000 INFO @ Sat, 15 Jan 2022 20:25:55: 14000000 INFO @ Sat, 15 Jan 2022 20:25:58: 20000000 INFO @ Sat, 15 Jan 2022 20:25:58: 10000000 INFO @ Sat, 15 Jan 2022 20:26:03: 15000000 INFO @ Sat, 15 Jan 2022 20:26:05: 21000000 INFO @ Sat, 15 Jan 2022 20:26:05: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:26:10: 16000000 INFO @ Sat, 15 Jan 2022 20:26:12: 22000000 INFO @ Sat, 15 Jan 2022 20:26:13: 12000000 INFO @ Sat, 15 Jan 2022 20:26:17: 17000000 INFO @ Sat, 15 Jan 2022 20:26:20: 23000000 INFO @ Sat, 15 Jan 2022 20:26:20: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:26:24: 18000000 INFO @ Sat, 15 Jan 2022 20:26:27: 24000000 INFO @ Sat, 15 Jan 2022 20:26:27: 14000000 INFO @ Sat, 15 Jan 2022 20:26:32: 19000000 INFO @ Sat, 15 Jan 2022 20:26:34: 25000000 INFO @ Sat, 15 Jan 2022 20:26:34: 15000000 INFO @ Sat, 15 Jan 2022 20:26:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:26:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:26:36: #1 total tags in treatment: 9489389 INFO @ Sat, 15 Jan 2022 20:26:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:37: #1 tags after filtering in treatment: 7099420 INFO @ Sat, 15 Jan 2022 20:26:37: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:26:37: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:26:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 30 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:26:39: 20000000 INFO @ Sat, 15 Jan 2022 20:26:40: 16000000 INFO @ Sat, 15 Jan 2022 20:26:45: 21000000 INFO @ Sat, 15 Jan 2022 20:26:47: 17000000 INFO @ Sat, 15 Jan 2022 20:26:52: 22000000 INFO @ Sat, 15 Jan 2022 20:26:54: 18000000 INFO @ Sat, 15 Jan 2022 20:26:59: 23000000 INFO @ Sat, 15 Jan 2022 20:27:00: 19000000 INFO @ Sat, 15 Jan 2022 20:27:06: 24000000 INFO @ Sat, 15 Jan 2022 20:27:07: 20000000 INFO @ Sat, 15 Jan 2022 20:27:12: 25000000 INFO @ Sat, 15 Jan 2022 20:27:14: 21000000 INFO @ Sat, 15 Jan 2022 20:27:15: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:27:15: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:27:15: #1 total tags in treatment: 9489389 INFO @ Sat, 15 Jan 2022 20:27:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:27:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:27:15: #1 tags after filtering in treatment: 7099420 INFO @ Sat, 15 Jan 2022 20:27:15: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:27:15: #1 finished! INFO @ Sat, 15 Jan 2022 20:27:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:27:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:27:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:27:20: 22000000 INFO @ Sat, 15 Jan 2022 20:27:26: 23000000 INFO @ Sat, 15 Jan 2022 20:27:32: 24000000 INFO @ Sat, 15 Jan 2022 20:27:37: 25000000 INFO @ Sat, 15 Jan 2022 20:27:40: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:27:40: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:27:40: #1 total tags in treatment: 9489389 INFO @ Sat, 15 Jan 2022 20:27:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:27:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:27:40: #1 tags after filtering in treatment: 7099420 INFO @ Sat, 15 Jan 2022 20:27:40: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:27:40: #1 finished! INFO @ Sat, 15 Jan 2022 20:27:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:27:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:27:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245425/SRX8245425.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling