Job ID = 14520822 SRX = SRX8245419 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8938026 spots for SRR11684630/SRR11684630.sra Written 8938026 spots for SRR11684630/SRR11684630.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:34 8938026 reads; of these: 8938026 (100.00%) were paired; of these: 3875213 (43.36%) aligned concordantly 0 times 4502008 (50.37%) aligned concordantly exactly 1 time 560805 (6.27%) aligned concordantly >1 times ---- 3875213 pairs aligned concordantly 0 times; of these: 11482 (0.30%) aligned discordantly 1 time ---- 3863731 pairs aligned 0 times concordantly or discordantly; of these: 7727462 mates make up the pairs; of these: 4316561 (55.86%) aligned 0 times 3013278 (38.99%) aligned exactly 1 time 397623 (5.15%) aligned >1 times 75.85% overall alignment rate Time searching: 00:08:34 Overall time: 00:08:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 74071 / 5073737 = 0.0146 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:14:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:14:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:14:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:14:58: 1000000 INFO @ Sat, 15 Jan 2022 20:15:13: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:23: 3000000 INFO @ Sat, 15 Jan 2022 20:15:29: 1000000 INFO @ Sat, 15 Jan 2022 20:15:32: 4000000 INFO @ Sat, 15 Jan 2022 20:15:38: 2000000 INFO @ Sat, 15 Jan 2022 20:15:42: 5000000 INFO @ Sat, 15 Jan 2022 20:15:45: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:52: 4000000 INFO @ Sat, 15 Jan 2022 20:15:52: 6000000 INFO @ Sat, 15 Jan 2022 20:15:58: 1000000 INFO @ Sat, 15 Jan 2022 20:15:59: 5000000 INFO @ Sat, 15 Jan 2022 20:16:02: 7000000 INFO @ Sat, 15 Jan 2022 20:16:06: 6000000 INFO @ Sat, 15 Jan 2022 20:16:07: 2000000 INFO @ Sat, 15 Jan 2022 20:16:12: 8000000 INFO @ Sat, 15 Jan 2022 20:16:14: 7000000 INFO @ Sat, 15 Jan 2022 20:16:16: 3000000 INFO @ Sat, 15 Jan 2022 20:16:21: 8000000 INFO @ Sat, 15 Jan 2022 20:16:21: 9000000 INFO @ Sat, 15 Jan 2022 20:16:27: 4000000 INFO @ Sat, 15 Jan 2022 20:16:28: 9000000 INFO @ Sat, 15 Jan 2022 20:16:31: 10000000 INFO @ Sat, 15 Jan 2022 20:16:35: 10000000 INFO @ Sat, 15 Jan 2022 20:16:36: 5000000 INFO @ Sat, 15 Jan 2022 20:16:41: 11000000 INFO @ Sat, 15 Jan 2022 20:16:42: 11000000 INFO @ Sat, 15 Jan 2022 20:16:45: 6000000 INFO @ Sat, 15 Jan 2022 20:16:49: 12000000 INFO @ Sat, 15 Jan 2022 20:16:50: 12000000 INFO @ Sat, 15 Jan 2022 20:16:54: 7000000 INFO @ Sat, 15 Jan 2022 20:16:56: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:16:59: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:16:59: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:16:59: #1 total tags in treatment: 4988762 INFO @ Sat, 15 Jan 2022 20:16:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:59: #1 tags after filtering in treatment: 4096463 INFO @ Sat, 15 Jan 2022 20:16:59: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:16:59: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:59: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 20:16:59: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.10_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 20:17:00: 13000000 pass1 - making usageList (0 chroms): 739 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:17:03: 8000000 INFO @ Sat, 15 Jan 2022 20:17:04: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:17:04: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:17:04: #1 total tags in treatment: 4988762 INFO @ Sat, 15 Jan 2022 20:17:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:04: #1 tags after filtering in treatment: 4096463 INFO @ Sat, 15 Jan 2022 20:17:04: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:17:04: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:05: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 20:17:05: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:17:12: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:17:21: 10000000 INFO @ Sat, 15 Jan 2022 20:17:30: 11000000 INFO @ Sat, 15 Jan 2022 20:17:39: 12000000 INFO @ Sat, 15 Jan 2022 20:17:48: 13000000 INFO @ Sat, 15 Jan 2022 20:17:52: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:17:52: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:17:52: #1 total tags in treatment: 4988762 INFO @ Sat, 15 Jan 2022 20:17:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:52: #1 tags after filtering in treatment: 4096463 INFO @ Sat, 15 Jan 2022 20:17:52: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:17:52: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:52: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 20:17:52: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245419/SRX8245419.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling