Job ID = 14520819 SRX = SRX8245416 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8911482 spots for SRR11684627/SRR11684627.sra Written 8911482 spots for SRR11684627/SRR11684627.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:05 8911482 reads; of these: 8911482 (100.00%) were paired; of these: 3838703 (43.08%) aligned concordantly 0 times 4483652 (50.31%) aligned concordantly exactly 1 time 589127 (6.61%) aligned concordantly >1 times ---- 3838703 pairs aligned concordantly 0 times; of these: 8754 (0.23%) aligned discordantly 1 time ---- 3829949 pairs aligned 0 times concordantly or discordantly; of these: 7659898 mates make up the pairs; of these: 4283477 (55.92%) aligned 0 times 2966259 (38.72%) aligned exactly 1 time 410162 (5.35%) aligned >1 times 75.97% overall alignment rate Time searching: 00:07:05 Overall time: 00:07:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 71013 / 5081004 = 0.0140 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:09:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:09:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:09:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:09:16: 1000000 INFO @ Sat, 15 Jan 2022 20:09:21: 2000000 INFO @ Sat, 15 Jan 2022 20:09:27: 3000000 INFO @ Sat, 15 Jan 2022 20:09:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:09:38: 5000000 INFO @ Sat, 15 Jan 2022 20:09:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:09:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:09:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:09:44: 6000000 INFO @ Sat, 15 Jan 2022 20:09:46: 1000000 INFO @ Sat, 15 Jan 2022 20:09:51: 7000000 INFO @ Sat, 15 Jan 2022 20:09:53: 2000000 INFO @ Sat, 15 Jan 2022 20:09:57: 8000000 INFO @ Sat, 15 Jan 2022 20:09:59: 3000000 INFO @ Sat, 15 Jan 2022 20:10:04: 9000000 INFO @ Sat, 15 Jan 2022 20:10:05: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:10:10: 10000000 INFO @ Sat, 15 Jan 2022 20:10:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:10:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:10:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:10:12: 5000000 INFO @ Sat, 15 Jan 2022 20:10:17: 11000000 INFO @ Sat, 15 Jan 2022 20:10:18: 1000000 INFO @ Sat, 15 Jan 2022 20:10:18: 6000000 INFO @ Sat, 15 Jan 2022 20:10:24: 12000000 INFO @ Sat, 15 Jan 2022 20:10:25: 2000000 INFO @ Sat, 15 Jan 2022 20:10:27: 7000000 INFO @ Sat, 15 Jan 2022 20:10:31: 13000000 INFO @ Sat, 15 Jan 2022 20:10:33: 3000000 INFO @ Sat, 15 Jan 2022 20:10:34: 8000000 INFO @ Sat, 15 Jan 2022 20:10:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:10:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:10:34: #1 total tags in treatment: 5001775 INFO @ Sat, 15 Jan 2022 20:10:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:10:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:10:34: #1 tags after filtering in treatment: 4093459 INFO @ Sat, 15 Jan 2022 20:10:34: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:10:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:10:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:10:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:10:35: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 20:10:35: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:10:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:10:41: 4000000 INFO @ Sat, 15 Jan 2022 20:10:41: 9000000 INFO @ Sat, 15 Jan 2022 20:10:48: 10000000 INFO @ Sat, 15 Jan 2022 20:10:49: 5000000 INFO @ Sat, 15 Jan 2022 20:10:56: 11000000 INFO @ Sat, 15 Jan 2022 20:10:58: 6000000 INFO @ Sat, 15 Jan 2022 20:11:03: 12000000 INFO @ Sat, 15 Jan 2022 20:11:05: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:11:11: 13000000 INFO @ Sat, 15 Jan 2022 20:11:13: 8000000 INFO @ Sat, 15 Jan 2022 20:11:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:11:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:11:14: #1 total tags in treatment: 5001775 INFO @ Sat, 15 Jan 2022 20:11:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:11:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:11:14: #1 tags after filtering in treatment: 4093459 INFO @ Sat, 15 Jan 2022 20:11:14: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:11:14: #1 finished! INFO @ Sat, 15 Jan 2022 20:11:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:11:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:11:15: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 20:11:15: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:11:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:11:19: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:11:25: 10000000 INFO @ Sat, 15 Jan 2022 20:11:31: 11000000 INFO @ Sat, 15 Jan 2022 20:11:37: 12000000 INFO @ Sat, 15 Jan 2022 20:11:43: 13000000 INFO @ Sat, 15 Jan 2022 20:11:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:11:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:11:46: #1 total tags in treatment: 5001775 INFO @ Sat, 15 Jan 2022 20:11:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:11:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:11:46: #1 tags after filtering in treatment: 4093459 INFO @ Sat, 15 Jan 2022 20:11:46: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:11:46: #1 finished! INFO @ Sat, 15 Jan 2022 20:11:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:11:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:11:46: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 20:11:46: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:11:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245416/SRX8245416.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling