Job ID = 14520815 SRX = SRX8245412 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8201820 spots for SRR11684623/SRR11684623.sra Written 8201820 spots for SRR11684623/SRR11684623.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:52 8201820 reads; of these: 8201820 (100.00%) were paired; of these: 3686829 (44.95%) aligned concordantly 0 times 4067050 (49.59%) aligned concordantly exactly 1 time 447941 (5.46%) aligned concordantly >1 times ---- 3686829 pairs aligned concordantly 0 times; of these: 43994 (1.19%) aligned discordantly 1 time ---- 3642835 pairs aligned 0 times concordantly or discordantly; of these: 7285670 mates make up the pairs; of these: 4007280 (55.00%) aligned 0 times 2918902 (40.06%) aligned exactly 1 time 359488 (4.93%) aligned >1 times 75.57% overall alignment rate Time searching: 00:04:52 Overall time: 00:04:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 38526 / 4558391 = 0.0085 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:04:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:04:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:04:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:04:42: 1000000 INFO @ Sat, 15 Jan 2022 20:04:47: 2000000 INFO @ Sat, 15 Jan 2022 20:04:52: 3000000 INFO @ Sat, 15 Jan 2022 20:04:57: 4000000 INFO @ Sat, 15 Jan 2022 20:05:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:05:06: 6000000 INFO @ Sat, 15 Jan 2022 20:05:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:05:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:05:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:05:11: 7000000 INFO @ Sat, 15 Jan 2022 20:05:13: 1000000 INFO @ Sat, 15 Jan 2022 20:05:16: 8000000 INFO @ Sat, 15 Jan 2022 20:05:18: 2000000 INFO @ Sat, 15 Jan 2022 20:05:22: 9000000 INFO @ Sat, 15 Jan 2022 20:05:24: 3000000 INFO @ Sat, 15 Jan 2022 20:05:27: 10000000 INFO @ Sat, 15 Jan 2022 20:05:29: 4000000 INFO @ Sat, 15 Jan 2022 20:05:32: 11000000 INFO @ Sat, 15 Jan 2022 20:05:34: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:05:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:05:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:05:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:05:38: 12000000 INFO @ Sat, 15 Jan 2022 20:05:39: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:05:39: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:05:39: #1 total tags in treatment: 4476555 INFO @ Sat, 15 Jan 2022 20:05:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:05:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:05:39: #1 tags after filtering in treatment: 3770051 INFO @ Sat, 15 Jan 2022 20:05:39: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 20:05:39: #1 finished! INFO @ Sat, 15 Jan 2022 20:05:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:05:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:05:40: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:05:40: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:05:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 20:05:40: 6000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:05:44: 1000000 INFO @ Sat, 15 Jan 2022 20:05:45: 7000000 INFO @ Sat, 15 Jan 2022 20:05:50: 2000000 INFO @ Sat, 15 Jan 2022 20:05:51: 8000000 INFO @ Sat, 15 Jan 2022 20:05:56: 3000000 INFO @ Sat, 15 Jan 2022 20:05:57: 9000000 INFO @ Sat, 15 Jan 2022 20:06:03: 10000000 INFO @ Sat, 15 Jan 2022 20:06:03: 4000000 INFO @ Sat, 15 Jan 2022 20:06:08: 11000000 INFO @ Sat, 15 Jan 2022 20:06:09: 5000000 INFO @ Sat, 15 Jan 2022 20:06:14: 12000000 INFO @ Sat, 15 Jan 2022 20:06:16: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:06:16: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:06:16: #1 total tags in treatment: 4476555 INFO @ Sat, 15 Jan 2022 20:06:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:06:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:06:16: #1 tags after filtering in treatment: 3770051 INFO @ Sat, 15 Jan 2022 20:06:16: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 20:06:16: #1 finished! INFO @ Sat, 15 Jan 2022 20:06:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:06:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:06:16: 6000000 INFO @ Sat, 15 Jan 2022 20:06:16: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:06:16: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:06:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:06:22: 7000000 INFO @ Sat, 15 Jan 2022 20:06:28: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:06:34: 9000000 INFO @ Sat, 15 Jan 2022 20:06:41: 10000000 INFO @ Sat, 15 Jan 2022 20:06:47: 11000000 INFO @ Sat, 15 Jan 2022 20:06:53: 12000000 INFO @ Sat, 15 Jan 2022 20:06:54: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:06:54: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:06:54: #1 total tags in treatment: 4476555 INFO @ Sat, 15 Jan 2022 20:06:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:06:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:06:54: #1 tags after filtering in treatment: 3770051 INFO @ Sat, 15 Jan 2022 20:06:54: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 20:06:54: #1 finished! INFO @ Sat, 15 Jan 2022 20:06:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:06:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:06:55: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:06:55: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:06:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245412/SRX8245412.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling