Job ID = 14520785 SRX = SRX8245403 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7853737 spots for SRR11684614/SRR11684614.sra Written 7853737 spots for SRR11684614/SRR11684614.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:14 7853737 reads; of these: 7853737 (100.00%) were paired; of these: 4639724 (59.08%) aligned concordantly 0 times 2930821 (37.32%) aligned concordantly exactly 1 time 283192 (3.61%) aligned concordantly >1 times ---- 4639724 pairs aligned concordantly 0 times; of these: 24279 (0.52%) aligned discordantly 1 time ---- 4615445 pairs aligned 0 times concordantly or discordantly; of these: 9230890 mates make up the pairs; of these: 4968216 (53.82%) aligned 0 times 3849103 (41.70%) aligned exactly 1 time 413571 (4.48%) aligned >1 times 68.37% overall alignment rate Time searching: 00:06:14 Overall time: 00:06:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 22019 / 3237617 = 0.0068 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:02:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:02:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:02:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:02:44: 1000000 INFO @ Sat, 15 Jan 2022 20:02:53: 2000000 INFO @ Sat, 15 Jan 2022 20:03:02: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:03:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:03:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:03:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:03:11: 4000000 INFO @ Sat, 15 Jan 2022 20:03:13: 1000000 INFO @ Sat, 15 Jan 2022 20:03:20: 5000000 INFO @ Sat, 15 Jan 2022 20:03:21: 2000000 INFO @ Sat, 15 Jan 2022 20:03:29: 6000000 INFO @ Sat, 15 Jan 2022 20:03:29: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:03:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:03:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:03:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:03:37: 4000000 INFO @ Sat, 15 Jan 2022 20:03:38: 7000000 INFO @ Sat, 15 Jan 2022 20:03:44: 1000000 INFO @ Sat, 15 Jan 2022 20:03:45: 5000000 INFO @ Sat, 15 Jan 2022 20:03:48: 8000000 INFO @ Sat, 15 Jan 2022 20:03:53: 6000000 INFO @ Sat, 15 Jan 2022 20:03:53: 2000000 INFO @ Sat, 15 Jan 2022 20:03:57: 9000000 INFO @ Sat, 15 Jan 2022 20:04:02: 7000000 INFO @ Sat, 15 Jan 2022 20:04:03: 3000000 INFO @ Sat, 15 Jan 2022 20:04:06: 10000000 INFO @ Sat, 15 Jan 2022 20:04:09: 8000000 INFO @ Sat, 15 Jan 2022 20:04:12: 4000000 INFO @ Sat, 15 Jan 2022 20:04:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:04:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:04:13: #1 total tags in treatment: 3192081 INFO @ Sat, 15 Jan 2022 20:04:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:04:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:04:13: #1 tags after filtering in treatment: 2816038 INFO @ Sat, 15 Jan 2022 20:04:13: #1 Redundant rate of treatment: 0.12 INFO @ Sat, 15 Jan 2022 20:04:13: #1 finished! INFO @ Sat, 15 Jan 2022 20:04:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:04:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:04:13: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 20:04:13: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:04:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:04:18: 9000000 INFO @ Sat, 15 Jan 2022 20:04:21: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:04:26: 10000000 INFO @ Sat, 15 Jan 2022 20:04:30: 6000000 INFO @ Sat, 15 Jan 2022 20:04:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:04:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:04:31: #1 total tags in treatment: 3192081 INFO @ Sat, 15 Jan 2022 20:04:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:04:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:04:31: #1 tags after filtering in treatment: 2816038 INFO @ Sat, 15 Jan 2022 20:04:31: #1 Redundant rate of treatment: 0.12 INFO @ Sat, 15 Jan 2022 20:04:31: #1 finished! INFO @ Sat, 15 Jan 2022 20:04:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:04:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:04:32: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 20:04:32: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:04:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:04:39: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:04:48: 8000000 INFO @ Sat, 15 Jan 2022 20:04:57: 9000000 INFO @ Sat, 15 Jan 2022 20:05:06: 10000000 INFO @ Sat, 15 Jan 2022 20:05:12: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:05:12: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:05:12: #1 total tags in treatment: 3192081 INFO @ Sat, 15 Jan 2022 20:05:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:05:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:05:12: #1 tags after filtering in treatment: 2816038 INFO @ Sat, 15 Jan 2022 20:05:12: #1 Redundant rate of treatment: 0.12 INFO @ Sat, 15 Jan 2022 20:05:12: #1 finished! INFO @ Sat, 15 Jan 2022 20:05:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:05:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:05:12: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 20:05:12: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:05:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245403/SRX8245403.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling