Job ID = 14521201 SRX = SRX8245379 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11908602 spots for SRR11684590/SRR11684590.sra Written 11908602 spots for SRR11684590/SRR11684590.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:22 11908602 reads; of these: 11908602 (100.00%) were paired; of these: 5929613 (49.79%) aligned concordantly 0 times 3958907 (33.24%) aligned concordantly exactly 1 time 2020082 (16.96%) aligned concordantly >1 times ---- 5929613 pairs aligned concordantly 0 times; of these: 185862 (3.13%) aligned discordantly 1 time ---- 5743751 pairs aligned 0 times concordantly or discordantly; of these: 11487502 mates make up the pairs; of these: 7383176 (64.27%) aligned 0 times 2673176 (23.27%) aligned exactly 1 time 1431150 (12.46%) aligned >1 times 69.00% overall alignment rate Time searching: 00:13:22 Overall time: 00:13:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 563707 / 6163554 = 0.0915 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:55:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:55:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:55:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:55:17: 1000000 INFO @ Sat, 15 Jan 2022 20:55:22: 2000000 INFO @ Sat, 15 Jan 2022 20:55:26: 3000000 INFO @ Sat, 15 Jan 2022 20:55:30: 4000000 INFO @ Sat, 15 Jan 2022 20:55:35: 5000000 INFO @ Sat, 15 Jan 2022 20:55:39: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:55:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:55:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:55:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:55:43: 7000000 INFO @ Sat, 15 Jan 2022 20:55:47: 1000000 INFO @ Sat, 15 Jan 2022 20:55:48: 8000000 INFO @ Sat, 15 Jan 2022 20:55:52: 2000000 INFO @ Sat, 15 Jan 2022 20:55:53: 9000000 INFO @ Sat, 15 Jan 2022 20:55:57: 3000000 INFO @ Sat, 15 Jan 2022 20:55:58: 10000000 INFO @ Sat, 15 Jan 2022 20:56:02: 4000000 INFO @ Sat, 15 Jan 2022 20:56:03: 11000000 INFO @ Sat, 15 Jan 2022 20:56:07: 5000000 INFO @ Sat, 15 Jan 2022 20:56:08: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:56:11: 6000000 INFO @ Sat, 15 Jan 2022 20:56:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:56:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:56:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:56:13: 13000000 INFO @ Sat, 15 Jan 2022 20:56:16: 7000000 INFO @ Sat, 15 Jan 2022 20:56:17: 1000000 INFO @ Sat, 15 Jan 2022 20:56:18: 14000000 INFO @ Sat, 15 Jan 2022 20:56:21: 8000000 INFO @ Sat, 15 Jan 2022 20:56:22: 2000000 INFO @ Sat, 15 Jan 2022 20:56:23: 15000000 INFO @ Sat, 15 Jan 2022 20:56:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:56:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:56:24: #1 total tags in treatment: 5425294 INFO @ Sat, 15 Jan 2022 20:56:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:56:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:56:24: #1 tags after filtering in treatment: 3214262 INFO @ Sat, 15 Jan 2022 20:56:24: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:56:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:56:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:56:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:56:25: #2 number of paired peaks: 36 WARNING @ Sat, 15 Jan 2022 20:56:25: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:56:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:56:26: 9000000 INFO @ Sat, 15 Jan 2022 20:56:27: 3000000 INFO @ Sat, 15 Jan 2022 20:56:30: 10000000 INFO @ Sat, 15 Jan 2022 20:56:32: 4000000 INFO @ Sat, 15 Jan 2022 20:56:35: 11000000 INFO @ Sat, 15 Jan 2022 20:56:36: 5000000 INFO @ Sat, 15 Jan 2022 20:56:40: 12000000 INFO @ Sat, 15 Jan 2022 20:56:41: 6000000 INFO @ Sat, 15 Jan 2022 20:56:44: 13000000 INFO @ Sat, 15 Jan 2022 20:56:46: 7000000 INFO @ Sat, 15 Jan 2022 20:56:49: 14000000 INFO @ Sat, 15 Jan 2022 20:56:51: 8000000 INFO @ Sat, 15 Jan 2022 20:56:54: 15000000 INFO @ Sat, 15 Jan 2022 20:56:55: 9000000 INFO @ Sat, 15 Jan 2022 20:56:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:56:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:56:56: #1 total tags in treatment: 5425294 INFO @ Sat, 15 Jan 2022 20:56:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:56:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:56:56: #1 tags after filtering in treatment: 3214262 INFO @ Sat, 15 Jan 2022 20:56:56: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:56:56: #1 finished! INFO @ Sat, 15 Jan 2022 20:56:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:56:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:56:56: #2 number of paired peaks: 36 WARNING @ Sat, 15 Jan 2022 20:56:56: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:56:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:57:00: 10000000 INFO @ Sat, 15 Jan 2022 20:57:05: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:57:09: 12000000 INFO @ Sat, 15 Jan 2022 20:57:14: 13000000 INFO @ Sat, 15 Jan 2022 20:57:19: 14000000 INFO @ Sat, 15 Jan 2022 20:57:23: 15000000 INFO @ Sat, 15 Jan 2022 20:57:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:57:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:57:25: #1 total tags in treatment: 5425294 INFO @ Sat, 15 Jan 2022 20:57:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:57:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:57:25: #1 tags after filtering in treatment: 3214262 INFO @ Sat, 15 Jan 2022 20:57:25: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:57:25: #1 finished! INFO @ Sat, 15 Jan 2022 20:57:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:57:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:57:25: #2 number of paired peaks: 36 WARNING @ Sat, 15 Jan 2022 20:57:25: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:57:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245379/SRX8245379.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling