Job ID = 14521195 SRX = SRX8245373 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12132936 spots for SRR11684584/SRR11684584.sra Written 12132936 spots for SRR11684584/SRR11684584.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:35 12132936 reads; of these: 12132936 (100.00%) were paired; of these: 5224746 (43.06%) aligned concordantly 0 times 5635459 (46.45%) aligned concordantly exactly 1 time 1272731 (10.49%) aligned concordantly >1 times ---- 5224746 pairs aligned concordantly 0 times; of these: 58821 (1.13%) aligned discordantly 1 time ---- 5165925 pairs aligned 0 times concordantly or discordantly; of these: 10331850 mates make up the pairs; of these: 5809945 (56.23%) aligned 0 times 3650893 (35.34%) aligned exactly 1 time 871012 (8.43%) aligned >1 times 76.06% overall alignment rate Time searching: 00:07:35 Overall time: 00:07:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 250201 / 6966226 = 0.0359 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:50:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:50:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:50:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:50:19: 1000000 INFO @ Sat, 15 Jan 2022 20:50:24: 2000000 INFO @ Sat, 15 Jan 2022 20:50:28: 3000000 INFO @ Sat, 15 Jan 2022 20:50:32: 4000000 INFO @ Sat, 15 Jan 2022 20:50:36: 5000000 INFO @ Sat, 15 Jan 2022 20:50:40: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:50:45: 7000000 INFO @ Sat, 15 Jan 2022 20:50:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:50:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:50:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:50:49: 8000000 INFO @ Sat, 15 Jan 2022 20:50:49: 1000000 INFO @ Sat, 15 Jan 2022 20:50:54: 9000000 INFO @ Sat, 15 Jan 2022 20:50:54: 2000000 INFO @ Sat, 15 Jan 2022 20:50:58: 10000000 INFO @ Sat, 15 Jan 2022 20:50:59: 3000000 INFO @ Sat, 15 Jan 2022 20:51:03: 11000000 INFO @ Sat, 15 Jan 2022 20:51:03: 4000000 INFO @ Sat, 15 Jan 2022 20:51:07: 12000000 INFO @ Sat, 15 Jan 2022 20:51:07: 5000000 INFO @ Sat, 15 Jan 2022 20:51:12: 13000000 INFO @ Sat, 15 Jan 2022 20:51:12: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:51:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:51:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:51:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:51:16: 14000000 INFO @ Sat, 15 Jan 2022 20:51:16: 7000000 INFO @ Sat, 15 Jan 2022 20:51:20: 1000000 INFO @ Sat, 15 Jan 2022 20:51:21: 15000000 INFO @ Sat, 15 Jan 2022 20:51:21: 8000000 INFO @ Sat, 15 Jan 2022 20:51:25: 16000000 INFO @ Sat, 15 Jan 2022 20:51:25: 9000000 INFO @ Sat, 15 Jan 2022 20:51:25: 2000000 INFO @ Sat, 15 Jan 2022 20:51:30: 17000000 INFO @ Sat, 15 Jan 2022 20:51:30: 10000000 INFO @ Sat, 15 Jan 2022 20:51:31: 3000000 INFO @ Sat, 15 Jan 2022 20:51:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:51:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:51:34: #1 total tags in treatment: 6658395 INFO @ Sat, 15 Jan 2022 20:51:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:51:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:51:35: #1 tags after filtering in treatment: 4649313 INFO @ Sat, 15 Jan 2022 20:51:35: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:51:35: #1 finished! INFO @ Sat, 15 Jan 2022 20:51:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:51:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:51:35: 11000000 INFO @ Sat, 15 Jan 2022 20:51:35: #2 number of paired peaks: 54 WARNING @ Sat, 15 Jan 2022 20:51:35: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:51:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:51:36: 4000000 INFO @ Sat, 15 Jan 2022 20:51:39: 12000000 INFO @ Sat, 15 Jan 2022 20:51:41: 5000000 INFO @ Sat, 15 Jan 2022 20:51:43: 13000000 INFO @ Sat, 15 Jan 2022 20:51:47: 6000000 INFO @ Sat, 15 Jan 2022 20:51:48: 14000000 INFO @ Sat, 15 Jan 2022 20:51:52: 7000000 INFO @ Sat, 15 Jan 2022 20:51:52: 15000000 INFO @ Sat, 15 Jan 2022 20:51:57: 16000000 INFO @ Sat, 15 Jan 2022 20:51:58: 8000000 INFO @ Sat, 15 Jan 2022 20:52:02: 17000000 INFO @ Sat, 15 Jan 2022 20:52:03: 9000000 INFO @ Sat, 15 Jan 2022 20:52:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:52:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:52:06: #1 total tags in treatment: 6658395 INFO @ Sat, 15 Jan 2022 20:52:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:52:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:52:06: #1 tags after filtering in treatment: 4649313 INFO @ Sat, 15 Jan 2022 20:52:06: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:52:06: #1 finished! INFO @ Sat, 15 Jan 2022 20:52:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:52:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:52:06: #2 number of paired peaks: 54 WARNING @ Sat, 15 Jan 2022 20:52:06: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:52:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:52:08: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:52:14: 11000000 INFO @ Sat, 15 Jan 2022 20:52:19: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:52:24: 13000000 INFO @ Sat, 15 Jan 2022 20:52:29: 14000000 INFO @ Sat, 15 Jan 2022 20:52:35: 15000000 INFO @ Sat, 15 Jan 2022 20:52:40: 16000000 INFO @ Sat, 15 Jan 2022 20:52:45: 17000000 INFO @ Sat, 15 Jan 2022 20:52:50: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:52:50: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:52:50: #1 total tags in treatment: 6658395 INFO @ Sat, 15 Jan 2022 20:52:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:52:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:52:50: #1 tags after filtering in treatment: 4649313 INFO @ Sat, 15 Jan 2022 20:52:50: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:52:50: #1 finished! INFO @ Sat, 15 Jan 2022 20:52:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:52:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:52:51: #2 number of paired peaks: 54 WARNING @ Sat, 15 Jan 2022 20:52:51: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:52:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245373/SRX8245373.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling