Job ID = 14521141 SRX = SRX8245367 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18913627 spots for SRR11684578/SRR11684578.sra Written 18913627 spots for SRR11684578/SRR11684578.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:57 18913627 reads; of these: 18913627 (100.00%) were paired; of these: 9154010 (48.40%) aligned concordantly 0 times 8180762 (43.25%) aligned concordantly exactly 1 time 1578855 (8.35%) aligned concordantly >1 times ---- 9154010 pairs aligned concordantly 0 times; of these: 137628 (1.50%) aligned discordantly 1 time ---- 9016382 pairs aligned 0 times concordantly or discordantly; of these: 18032764 mates make up the pairs; of these: 11592987 (64.29%) aligned 0 times 5290044 (29.34%) aligned exactly 1 time 1149733 (6.38%) aligned >1 times 69.35% overall alignment rate Time searching: 00:14:57 Overall time: 00:14:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1051927 / 9895991 = 0.1063 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:59:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:59:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:59:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:59:11: 1000000 INFO @ Sat, 15 Jan 2022 20:59:17: 2000000 INFO @ Sat, 15 Jan 2022 20:59:22: 3000000 INFO @ Sat, 15 Jan 2022 20:59:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:59:34: 5000000 INFO @ Sat, 15 Jan 2022 20:59:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:59:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:59:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:59:39: 6000000 INFO @ Sat, 15 Jan 2022 20:59:42: 1000000 INFO @ Sat, 15 Jan 2022 20:59:45: 7000000 INFO @ Sat, 15 Jan 2022 20:59:48: 2000000 INFO @ Sat, 15 Jan 2022 20:59:51: 8000000 INFO @ Sat, 15 Jan 2022 20:59:54: 3000000 INFO @ Sat, 15 Jan 2022 20:59:57: 9000000 INFO @ Sat, 15 Jan 2022 21:00:01: 4000000 INFO @ Sat, 15 Jan 2022 21:00:03: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:00:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:00:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:00:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:00:07: 5000000 INFO @ Sat, 15 Jan 2022 21:00:09: 11000000 INFO @ Sat, 15 Jan 2022 21:00:13: 6000000 INFO @ Sat, 15 Jan 2022 21:00:13: 1000000 INFO @ Sat, 15 Jan 2022 21:00:15: 12000000 INFO @ Sat, 15 Jan 2022 21:00:19: 7000000 INFO @ Sat, 15 Jan 2022 21:00:21: 2000000 INFO @ Sat, 15 Jan 2022 21:00:22: 13000000 INFO @ Sat, 15 Jan 2022 21:00:25: 8000000 INFO @ Sat, 15 Jan 2022 21:00:28: 14000000 INFO @ Sat, 15 Jan 2022 21:00:29: 3000000 INFO @ Sat, 15 Jan 2022 21:00:31: 9000000 INFO @ Sat, 15 Jan 2022 21:00:35: 15000000 INFO @ Sat, 15 Jan 2022 21:00:36: 4000000 INFO @ Sat, 15 Jan 2022 21:00:37: 10000000 INFO @ Sat, 15 Jan 2022 21:00:42: 16000000 INFO @ Sat, 15 Jan 2022 21:00:44: 5000000 INFO @ Sat, 15 Jan 2022 21:00:44: 11000000 INFO @ Sat, 15 Jan 2022 21:00:48: 17000000 INFO @ Sat, 15 Jan 2022 21:00:51: 12000000 INFO @ Sat, 15 Jan 2022 21:00:51: 6000000 INFO @ Sat, 15 Jan 2022 21:00:54: 18000000 INFO @ Sat, 15 Jan 2022 21:00:57: 13000000 INFO @ Sat, 15 Jan 2022 21:00:58: 7000000 INFO @ Sat, 15 Jan 2022 21:01:01: 19000000 INFO @ Sat, 15 Jan 2022 21:01:04: 14000000 INFO @ Sat, 15 Jan 2022 21:01:06: 8000000 INFO @ Sat, 15 Jan 2022 21:01:08: 20000000 INFO @ Sat, 15 Jan 2022 21:01:11: 15000000 INFO @ Sat, 15 Jan 2022 21:01:14: 9000000 INFO @ Sat, 15 Jan 2022 21:01:15: 21000000 INFO @ Sat, 15 Jan 2022 21:01:19: 16000000 INFO @ Sat, 15 Jan 2022 21:01:21: 10000000 INFO @ Sat, 15 Jan 2022 21:01:22: 22000000 INFO @ Sat, 15 Jan 2022 21:01:25: 17000000 INFO @ Sat, 15 Jan 2022 21:01:29: 11000000 INFO @ Sat, 15 Jan 2022 21:01:30: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:01:32: 18000000 INFO @ Sat, 15 Jan 2022 21:01:37: 24000000 INFO @ Sat, 15 Jan 2022 21:01:37: 12000000 INFO @ Sat, 15 Jan 2022 21:01:38: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:01:38: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:01:38: #1 total tags in treatment: 8712552 INFO @ Sat, 15 Jan 2022 21:01:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:01:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:01:38: #1 tags after filtering in treatment: 5343203 INFO @ Sat, 15 Jan 2022 21:01:38: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 21:01:38: #1 finished! INFO @ Sat, 15 Jan 2022 21:01:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:01:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:01:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:01:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:01:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:01:39: 19000000 INFO @ Sat, 15 Jan 2022 21:01:45: 13000000 INFO @ Sat, 15 Jan 2022 21:01:47: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:01:54: 14000000 INFO @ Sat, 15 Jan 2022 21:01:54: 21000000 INFO @ Sat, 15 Jan 2022 21:02:01: 22000000 INFO @ Sat, 15 Jan 2022 21:02:02: 15000000 INFO @ Sat, 15 Jan 2022 21:02:08: 23000000 INFO @ Sat, 15 Jan 2022 21:02:11: 16000000 INFO @ Sat, 15 Jan 2022 21:02:16: 24000000 INFO @ Sat, 15 Jan 2022 21:02:17: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:02:17: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:02:17: #1 total tags in treatment: 8712552 INFO @ Sat, 15 Jan 2022 21:02:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:02:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:02:17: #1 tags after filtering in treatment: 5343203 INFO @ Sat, 15 Jan 2022 21:02:17: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 21:02:17: #1 finished! INFO @ Sat, 15 Jan 2022 21:02:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:02:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:02:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:02:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:02:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:02:20: 17000000 INFO @ Sat, 15 Jan 2022 21:02:28: 18000000 INFO @ Sat, 15 Jan 2022 21:02:36: 19000000 INFO @ Sat, 15 Jan 2022 21:02:45: 20000000 INFO @ Sat, 15 Jan 2022 21:02:53: 21000000 INFO @ Sat, 15 Jan 2022 21:03:01: 22000000 INFO @ Sat, 15 Jan 2022 21:03:08: 23000000 INFO @ Sat, 15 Jan 2022 21:03:14: 24000000 INFO @ Sat, 15 Jan 2022 21:03:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:03:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:03:14: #1 total tags in treatment: 8712552 INFO @ Sat, 15 Jan 2022 21:03:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:03:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:03:15: #1 tags after filtering in treatment: 5343203 INFO @ Sat, 15 Jan 2022 21:03:15: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 21:03:15: #1 finished! INFO @ Sat, 15 Jan 2022 21:03:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:03:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:03:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:03:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:03:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245367/SRX8245367.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling