Job ID = 14521137 SRX = SRX8245363 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12008050 spots for SRR11684574/SRR11684574.sra Written 12008050 spots for SRR11684574/SRR11684574.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:47 12008050 reads; of these: 12008050 (100.00%) were paired; of these: 4844431 (40.34%) aligned concordantly 0 times 5801946 (48.32%) aligned concordantly exactly 1 time 1361673 (11.34%) aligned concordantly >1 times ---- 4844431 pairs aligned concordantly 0 times; of these: 87579 (1.81%) aligned discordantly 1 time ---- 4756852 pairs aligned 0 times concordantly or discordantly; of these: 9513704 mates make up the pairs; of these: 5576081 (58.61%) aligned 0 times 3141049 (33.02%) aligned exactly 1 time 796574 (8.37%) aligned >1 times 76.78% overall alignment rate Time searching: 00:09:47 Overall time: 00:09:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 315562 / 7250289 = 0.0435 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:48:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:48:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:48:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:48:16: 1000000 INFO @ Sat, 15 Jan 2022 20:48:24: 2000000 INFO @ Sat, 15 Jan 2022 20:48:31: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:48:38: 4000000 INFO @ Sat, 15 Jan 2022 20:48:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:48:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:48:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:48:45: 5000000 INFO @ Sat, 15 Jan 2022 20:48:47: 1000000 INFO @ Sat, 15 Jan 2022 20:48:54: 6000000 INFO @ Sat, 15 Jan 2022 20:48:56: 2000000 INFO @ Sat, 15 Jan 2022 20:49:03: 7000000 INFO @ Sat, 15 Jan 2022 20:49:03: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:49:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:49:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:49:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:49:11: 4000000 INFO @ Sat, 15 Jan 2022 20:49:11: 8000000 INFO @ Sat, 15 Jan 2022 20:49:17: 1000000 INFO @ Sat, 15 Jan 2022 20:49:18: 5000000 INFO @ Sat, 15 Jan 2022 20:49:20: 9000000 INFO @ Sat, 15 Jan 2022 20:49:25: 2000000 INFO @ Sat, 15 Jan 2022 20:49:26: 6000000 INFO @ Sat, 15 Jan 2022 20:49:29: 10000000 INFO @ Sat, 15 Jan 2022 20:49:33: 3000000 INFO @ Sat, 15 Jan 2022 20:49:33: 7000000 INFO @ Sat, 15 Jan 2022 20:49:38: 11000000 INFO @ Sat, 15 Jan 2022 20:49:40: 4000000 INFO @ Sat, 15 Jan 2022 20:49:41: 8000000 INFO @ Sat, 15 Jan 2022 20:49:46: 12000000 INFO @ Sat, 15 Jan 2022 20:49:48: 5000000 INFO @ Sat, 15 Jan 2022 20:49:48: 9000000 INFO @ Sat, 15 Jan 2022 20:49:55: 13000000 INFO @ Sat, 15 Jan 2022 20:49:55: 6000000 INFO @ Sat, 15 Jan 2022 20:49:56: 10000000 INFO @ Sat, 15 Jan 2022 20:50:03: 7000000 INFO @ Sat, 15 Jan 2022 20:50:04: 14000000 INFO @ Sat, 15 Jan 2022 20:50:04: 11000000 INFO @ Sat, 15 Jan 2022 20:50:11: 8000000 INFO @ Sat, 15 Jan 2022 20:50:11: 12000000 INFO @ Sat, 15 Jan 2022 20:50:12: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:50:18: 9000000 INFO @ Sat, 15 Jan 2022 20:50:19: 13000000 INFO @ Sat, 15 Jan 2022 20:50:21: 16000000 INFO @ Sat, 15 Jan 2022 20:50:26: 10000000 INFO @ Sat, 15 Jan 2022 20:50:26: 14000000 INFO @ Sat, 15 Jan 2022 20:50:29: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:50:34: 11000000 INFO @ Sat, 15 Jan 2022 20:50:34: 15000000 INFO @ Sat, 15 Jan 2022 20:50:35: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:50:35: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:50:35: #1 total tags in treatment: 6848894 INFO @ Sat, 15 Jan 2022 20:50:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:50:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:50:36: #1 tags after filtering in treatment: 4845828 INFO @ Sat, 15 Jan 2022 20:50:36: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 20:50:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:50:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:50:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:50:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:50:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:50:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:50:41: 12000000 INFO @ Sat, 15 Jan 2022 20:50:41: 16000000 INFO @ Sat, 15 Jan 2022 20:50:47: 17000000 INFO @ Sat, 15 Jan 2022 20:50:48: 13000000 INFO @ Sat, 15 Jan 2022 20:50:52: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:50:52: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:50:52: #1 total tags in treatment: 6848894 INFO @ Sat, 15 Jan 2022 20:50:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:50:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:50:52: #1 tags after filtering in treatment: 4845828 INFO @ Sat, 15 Jan 2022 20:50:52: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 20:50:52: #1 finished! INFO @ Sat, 15 Jan 2022 20:50:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:50:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:50:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:50:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:50:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:50:55: 14000000 INFO @ Sat, 15 Jan 2022 20:51:03: 15000000 INFO @ Sat, 15 Jan 2022 20:51:10: 16000000 INFO @ Sat, 15 Jan 2022 20:51:17: 17000000 INFO @ Sat, 15 Jan 2022 20:51:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:51:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:51:22: #1 total tags in treatment: 6848894 INFO @ Sat, 15 Jan 2022 20:51:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:51:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:51:22: #1 tags after filtering in treatment: 4845828 INFO @ Sat, 15 Jan 2022 20:51:22: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 20:51:22: #1 finished! INFO @ Sat, 15 Jan 2022 20:51:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:51:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:51:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:51:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:51:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245363/SRX8245363.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling