Job ID = 14521099 SRX = SRX8245359 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19371896 spots for SRR11684570/SRR11684570.sra Written 19371896 spots for SRR11684570/SRR11684570.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:06 19371896 reads; of these: 19371896 (100.00%) were paired; of these: 8695229 (44.89%) aligned concordantly 0 times 9180141 (47.39%) aligned concordantly exactly 1 time 1496526 (7.73%) aligned concordantly >1 times ---- 8695229 pairs aligned concordantly 0 times; of these: 219506 (2.52%) aligned discordantly 1 time ---- 8475723 pairs aligned 0 times concordantly or discordantly; of these: 16951446 mates make up the pairs; of these: 10580389 (62.42%) aligned 0 times 5343914 (31.52%) aligned exactly 1 time 1027143 (6.06%) aligned >1 times 72.69% overall alignment rate Time searching: 00:11:07 Overall time: 00:11:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 903210 / 10894843 = 0.0829 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:49:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:49:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:49:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:49:43: 1000000 INFO @ Sat, 15 Jan 2022 20:49:49: 2000000 INFO @ Sat, 15 Jan 2022 20:49:54: 3000000 INFO @ Sat, 15 Jan 2022 20:50:00: 4000000 INFO @ Sat, 15 Jan 2022 20:50:05: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:50:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:50:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:50:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:50:11: 6000000 INFO @ Sat, 15 Jan 2022 20:50:14: 1000000 INFO @ Sat, 15 Jan 2022 20:50:17: 7000000 INFO @ Sat, 15 Jan 2022 20:50:20: 2000000 INFO @ Sat, 15 Jan 2022 20:50:23: 8000000 INFO @ Sat, 15 Jan 2022 20:50:26: 3000000 INFO @ Sat, 15 Jan 2022 20:50:28: 9000000 INFO @ Sat, 15 Jan 2022 20:50:32: 4000000 INFO @ Sat, 15 Jan 2022 20:50:34: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:50:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:50:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:50:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:50:38: 5000000 INFO @ Sat, 15 Jan 2022 20:50:40: 11000000 INFO @ Sat, 15 Jan 2022 20:50:44: 1000000 INFO @ Sat, 15 Jan 2022 20:50:44: 6000000 INFO @ Sat, 15 Jan 2022 20:50:46: 12000000 INFO @ Sat, 15 Jan 2022 20:50:50: 2000000 INFO @ Sat, 15 Jan 2022 20:50:50: 7000000 INFO @ Sat, 15 Jan 2022 20:50:52: 13000000 INFO @ Sat, 15 Jan 2022 20:50:56: 3000000 INFO @ Sat, 15 Jan 2022 20:50:57: 8000000 INFO @ Sat, 15 Jan 2022 20:50:58: 14000000 INFO @ Sat, 15 Jan 2022 20:51:02: 4000000 INFO @ Sat, 15 Jan 2022 20:51:02: 9000000 INFO @ Sat, 15 Jan 2022 20:51:04: 15000000 INFO @ Sat, 15 Jan 2022 20:51:08: 5000000 INFO @ Sat, 15 Jan 2022 20:51:08: 10000000 INFO @ Sat, 15 Jan 2022 20:51:10: 16000000 INFO @ Sat, 15 Jan 2022 20:51:14: 6000000 INFO @ Sat, 15 Jan 2022 20:51:14: 11000000 INFO @ Sat, 15 Jan 2022 20:51:16: 17000000 INFO @ Sat, 15 Jan 2022 20:51:20: 7000000 INFO @ Sat, 15 Jan 2022 20:51:21: 12000000 INFO @ Sat, 15 Jan 2022 20:51:22: 18000000 INFO @ Sat, 15 Jan 2022 20:51:26: 8000000 INFO @ Sat, 15 Jan 2022 20:51:27: 13000000 INFO @ Sat, 15 Jan 2022 20:51:28: 19000000 INFO @ Sat, 15 Jan 2022 20:51:32: 9000000 INFO @ Sat, 15 Jan 2022 20:51:33: 14000000 INFO @ Sat, 15 Jan 2022 20:51:34: 20000000 INFO @ Sat, 15 Jan 2022 20:51:38: 10000000 INFO @ Sat, 15 Jan 2022 20:51:40: 15000000 INFO @ Sat, 15 Jan 2022 20:51:40: 21000000 INFO @ Sat, 15 Jan 2022 20:51:44: 11000000 INFO @ Sat, 15 Jan 2022 20:51:46: 22000000 INFO @ Sat, 15 Jan 2022 20:51:46: 16000000 INFO @ Sat, 15 Jan 2022 20:51:50: 12000000 INFO @ Sat, 15 Jan 2022 20:51:52: 17000000 INFO @ Sat, 15 Jan 2022 20:51:52: 23000000 INFO @ Sat, 15 Jan 2022 20:51:56: 13000000 INFO @ Sat, 15 Jan 2022 20:51:57: 18000000 INFO @ Sat, 15 Jan 2022 20:51:58: 24000000 INFO @ Sat, 15 Jan 2022 20:52:02: 14000000 INFO @ Sat, 15 Jan 2022 20:52:04: 19000000 INFO @ Sat, 15 Jan 2022 20:52:04: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:52:08: 15000000 INFO @ Sat, 15 Jan 2022 20:52:09: 20000000 INFO @ Sat, 15 Jan 2022 20:52:10: 26000000 INFO @ Sat, 15 Jan 2022 20:52:12: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:52:12: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:52:12: #1 total tags in treatment: 9779970 INFO @ Sat, 15 Jan 2022 20:52:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:52:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:52:13: #1 tags after filtering in treatment: 5904547 INFO @ Sat, 15 Jan 2022 20:52:13: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 20:52:13: #1 finished! INFO @ Sat, 15 Jan 2022 20:52:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:52:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:52:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:52:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:52:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:52:14: 16000000 INFO @ Sat, 15 Jan 2022 20:52:15: 21000000 INFO @ Sat, 15 Jan 2022 20:52:21: 17000000 INFO @ Sat, 15 Jan 2022 20:52:21: 22000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:52:27: 23000000 INFO @ Sat, 15 Jan 2022 20:52:27: 18000000 INFO @ Sat, 15 Jan 2022 20:52:32: 24000000 INFO @ Sat, 15 Jan 2022 20:52:33: 19000000 INFO @ Sat, 15 Jan 2022 20:52:38: 25000000 INFO @ Sat, 15 Jan 2022 20:52:39: 20000000 INFO @ Sat, 15 Jan 2022 20:52:44: 26000000 INFO @ Sat, 15 Jan 2022 20:52:45: 21000000 INFO @ Sat, 15 Jan 2022 20:52:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:52:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:52:46: #1 total tags in treatment: 9779970 INFO @ Sat, 15 Jan 2022 20:52:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:52:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:52:46: #1 tags after filtering in treatment: 5904547 INFO @ Sat, 15 Jan 2022 20:52:46: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 20:52:46: #1 finished! INFO @ Sat, 15 Jan 2022 20:52:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:52:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:52:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:52:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:52:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:52:51: 22000000 INFO @ Sat, 15 Jan 2022 20:52:56: 23000000 INFO @ Sat, 15 Jan 2022 20:53:02: 24000000 INFO @ Sat, 15 Jan 2022 20:53:08: 25000000 INFO @ Sat, 15 Jan 2022 20:53:13: 26000000 INFO @ Sat, 15 Jan 2022 20:53:16: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:53:16: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:53:16: #1 total tags in treatment: 9779970 INFO @ Sat, 15 Jan 2022 20:53:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:53:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:53:16: #1 tags after filtering in treatment: 5904547 INFO @ Sat, 15 Jan 2022 20:53:16: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 20:53:16: #1 finished! INFO @ Sat, 15 Jan 2022 20:53:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:53:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:53:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:53:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:53:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245359/SRX8245359.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling