Job ID = 14521089 SRX = SRX8245351 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18397997 spots for SRR11684562/SRR11684562.sra Written 18397997 spots for SRR11684562/SRR11684562.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:55 18397997 reads; of these: 18397997 (100.00%) were paired; of these: 8206001 (44.60%) aligned concordantly 0 times 8593891 (46.71%) aligned concordantly exactly 1 time 1598105 (8.69%) aligned concordantly >1 times ---- 8206001 pairs aligned concordantly 0 times; of these: 32143 (0.39%) aligned discordantly 1 time ---- 8173858 pairs aligned 0 times concordantly or discordantly; of these: 16347716 mates make up the pairs; of these: 8726462 (53.38%) aligned 0 times 6372837 (38.98%) aligned exactly 1 time 1248417 (7.64%) aligned >1 times 76.28% overall alignment rate Time searching: 00:10:55 Overall time: 00:10:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 339403 / 10222830 = 0.0332 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:09: 1000000 INFO @ Sat, 15 Jan 2022 20:46:14: 2000000 INFO @ Sat, 15 Jan 2022 20:46:19: 3000000 INFO @ Sat, 15 Jan 2022 20:46:24: 4000000 INFO @ Sat, 15 Jan 2022 20:46:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:33: 6000000 INFO @ Sat, 15 Jan 2022 20:46:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:38: 7000000 INFO @ Sat, 15 Jan 2022 20:46:38: 1000000 INFO @ Sat, 15 Jan 2022 20:46:43: 2000000 INFO @ Sat, 15 Jan 2022 20:46:43: 8000000 INFO @ Sat, 15 Jan 2022 20:46:47: 3000000 INFO @ Sat, 15 Jan 2022 20:46:48: 9000000 INFO @ Sat, 15 Jan 2022 20:46:51: 4000000 INFO @ Sat, 15 Jan 2022 20:46:53: 10000000 INFO @ Sat, 15 Jan 2022 20:46:56: 5000000 INFO @ Sat, 15 Jan 2022 20:46:58: 11000000 INFO @ Sat, 15 Jan 2022 20:47:01: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:47:03: 12000000 INFO @ Sat, 15 Jan 2022 20:47:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:47:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:47:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:47:06: 7000000 INFO @ Sat, 15 Jan 2022 20:47:08: 13000000 INFO @ Sat, 15 Jan 2022 20:47:09: 1000000 INFO @ Sat, 15 Jan 2022 20:47:10: 8000000 INFO @ Sat, 15 Jan 2022 20:47:13: 14000000 INFO @ Sat, 15 Jan 2022 20:47:14: 2000000 INFO @ Sat, 15 Jan 2022 20:47:15: 9000000 INFO @ Sat, 15 Jan 2022 20:47:18: 15000000 INFO @ Sat, 15 Jan 2022 20:47:19: 3000000 INFO @ Sat, 15 Jan 2022 20:47:19: 10000000 INFO @ Sat, 15 Jan 2022 20:47:24: 16000000 INFO @ Sat, 15 Jan 2022 20:47:24: 11000000 INFO @ Sat, 15 Jan 2022 20:47:24: 4000000 INFO @ Sat, 15 Jan 2022 20:47:29: 12000000 INFO @ Sat, 15 Jan 2022 20:47:29: 17000000 INFO @ Sat, 15 Jan 2022 20:47:29: 5000000 INFO @ Sat, 15 Jan 2022 20:47:33: 13000000 INFO @ Sat, 15 Jan 2022 20:47:34: 6000000 INFO @ Sat, 15 Jan 2022 20:47:34: 18000000 INFO @ Sat, 15 Jan 2022 20:47:37: 14000000 INFO @ Sat, 15 Jan 2022 20:47:39: 7000000 INFO @ Sat, 15 Jan 2022 20:47:39: 19000000 INFO @ Sat, 15 Jan 2022 20:47:42: 15000000 INFO @ Sat, 15 Jan 2022 20:47:44: 8000000 INFO @ Sat, 15 Jan 2022 20:47:44: 20000000 INFO @ Sat, 15 Jan 2022 20:47:46: 16000000 INFO @ Sat, 15 Jan 2022 20:47:49: 9000000 INFO @ Sat, 15 Jan 2022 20:47:50: 21000000 INFO @ Sat, 15 Jan 2022 20:47:50: 17000000 INFO @ Sat, 15 Jan 2022 20:47:54: 10000000 INFO @ Sat, 15 Jan 2022 20:47:55: 18000000 INFO @ Sat, 15 Jan 2022 20:47:55: 22000000 INFO @ Sat, 15 Jan 2022 20:47:59: 11000000 INFO @ Sat, 15 Jan 2022 20:47:59: 19000000 INFO @ Sat, 15 Jan 2022 20:48:00: 23000000 INFO @ Sat, 15 Jan 2022 20:48:04: 20000000 INFO @ Sat, 15 Jan 2022 20:48:04: 12000000 INFO @ Sat, 15 Jan 2022 20:48:05: 24000000 INFO @ Sat, 15 Jan 2022 20:48:09: 21000000 INFO @ Sat, 15 Jan 2022 20:48:09: 13000000 INFO @ Sat, 15 Jan 2022 20:48:11: 25000000 INFO @ Sat, 15 Jan 2022 20:48:13: 22000000 INFO @ Sat, 15 Jan 2022 20:48:14: 14000000 INFO @ Sat, 15 Jan 2022 20:48:16: 26000000 INFO @ Sat, 15 Jan 2022 20:48:17: 23000000 INFO @ Sat, 15 Jan 2022 20:48:19: 15000000 INFO @ Sat, 15 Jan 2022 20:48:21: 27000000 INFO @ Sat, 15 Jan 2022 20:48:22: 24000000 INFO @ Sat, 15 Jan 2022 20:48:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:48:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:48:23: #1 total tags in treatment: 9852678 INFO @ Sat, 15 Jan 2022 20:48:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:24: #1 tags after filtering in treatment: 7040653 INFO @ Sat, 15 Jan 2022 20:48:24: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 20:48:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:48:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 20:48:25: 16000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:48:26: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:48:30: 17000000 INFO @ Sat, 15 Jan 2022 20:48:31: 26000000 INFO @ Sat, 15 Jan 2022 20:48:35: 18000000 INFO @ Sat, 15 Jan 2022 20:48:35: 27000000 INFO @ Sat, 15 Jan 2022 20:48:37: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:48:37: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:48:37: #1 total tags in treatment: 9852678 INFO @ Sat, 15 Jan 2022 20:48:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:37: #1 tags after filtering in treatment: 7040653 INFO @ Sat, 15 Jan 2022 20:48:37: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 20:48:37: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:48:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:48:40: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:48:45: 20000000 INFO @ Sat, 15 Jan 2022 20:48:50: 21000000 INFO @ Sat, 15 Jan 2022 20:48:56: 22000000 INFO @ Sat, 15 Jan 2022 20:49:01: 23000000 INFO @ Sat, 15 Jan 2022 20:49:06: 24000000 INFO @ Sat, 15 Jan 2022 20:49:11: 25000000 INFO @ Sat, 15 Jan 2022 20:49:17: 26000000 INFO @ Sat, 15 Jan 2022 20:49:22: 27000000 INFO @ Sat, 15 Jan 2022 20:49:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:49:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:49:24: #1 total tags in treatment: 9852678 INFO @ Sat, 15 Jan 2022 20:49:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:49:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:49:24: #1 tags after filtering in treatment: 7040653 INFO @ Sat, 15 Jan 2022 20:49:24: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 20:49:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:49:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:49:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:49:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:49:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:49:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245351/SRX8245351.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling