Job ID = 14521088 SRX = SRX8245350 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19353994 spots for SRR11684561/SRR11684561.sra Written 19353994 spots for SRR11684561/SRR11684561.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:54 19353994 reads; of these: 19353994 (100.00%) were paired; of these: 10459342 (54.04%) aligned concordantly 0 times 7551733 (39.02%) aligned concordantly exactly 1 time 1342919 (6.94%) aligned concordantly >1 times ---- 10459342 pairs aligned concordantly 0 times; of these: 29949 (0.29%) aligned discordantly 1 time ---- 10429393 pairs aligned 0 times concordantly or discordantly; of these: 20858786 mates make up the pairs; of these: 11051353 (52.98%) aligned 0 times 8280889 (39.70%) aligned exactly 1 time 1526544 (7.32%) aligned >1 times 71.45% overall alignment rate Time searching: 00:10:54 Overall time: 00:10:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 254343 / 8923447 = 0.0285 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:45:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:45:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:45:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:03: 1000000 INFO @ Sat, 15 Jan 2022 20:46:08: 2000000 INFO @ Sat, 15 Jan 2022 20:46:13: 3000000 INFO @ Sat, 15 Jan 2022 20:46:18: 4000000 INFO @ Sat, 15 Jan 2022 20:46:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:28: 6000000 INFO @ Sat, 15 Jan 2022 20:46:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:33: 7000000 INFO @ Sat, 15 Jan 2022 20:46:34: 1000000 INFO @ Sat, 15 Jan 2022 20:46:39: 8000000 INFO @ Sat, 15 Jan 2022 20:46:40: 2000000 INFO @ Sat, 15 Jan 2022 20:46:44: 9000000 INFO @ Sat, 15 Jan 2022 20:46:47: 3000000 INFO @ Sat, 15 Jan 2022 20:46:50: 10000000 INFO @ Sat, 15 Jan 2022 20:46:53: 4000000 INFO @ Sat, 15 Jan 2022 20:46:56: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:59: 5000000 INFO @ Sat, 15 Jan 2022 20:47:01: 12000000 INFO @ Sat, 15 Jan 2022 20:47:05: 1000000 INFO @ Sat, 15 Jan 2022 20:47:05: 6000000 INFO @ Sat, 15 Jan 2022 20:47:07: 13000000 INFO @ Sat, 15 Jan 2022 20:47:11: 2000000 INFO @ Sat, 15 Jan 2022 20:47:11: 7000000 INFO @ Sat, 15 Jan 2022 20:47:13: 14000000 INFO @ Sat, 15 Jan 2022 20:47:17: 3000000 INFO @ Sat, 15 Jan 2022 20:47:17: 8000000 INFO @ Sat, 15 Jan 2022 20:47:18: 15000000 INFO @ Sat, 15 Jan 2022 20:47:23: 4000000 INFO @ Sat, 15 Jan 2022 20:47:24: 9000000 INFO @ Sat, 15 Jan 2022 20:47:24: 16000000 INFO @ Sat, 15 Jan 2022 20:47:29: 5000000 INFO @ Sat, 15 Jan 2022 20:47:30: 17000000 INFO @ Sat, 15 Jan 2022 20:47:30: 10000000 INFO @ Sat, 15 Jan 2022 20:47:35: 18000000 INFO @ Sat, 15 Jan 2022 20:47:36: 6000000 INFO @ Sat, 15 Jan 2022 20:47:36: 11000000 INFO @ Sat, 15 Jan 2022 20:47:41: 19000000 INFO @ Sat, 15 Jan 2022 20:47:42: 7000000 INFO @ Sat, 15 Jan 2022 20:47:43: 12000000 INFO @ Sat, 15 Jan 2022 20:47:46: 20000000 INFO @ Sat, 15 Jan 2022 20:47:48: 8000000 INFO @ Sat, 15 Jan 2022 20:47:49: 13000000 INFO @ Sat, 15 Jan 2022 20:47:52: 21000000 INFO @ Sat, 15 Jan 2022 20:47:54: 9000000 INFO @ Sat, 15 Jan 2022 20:47:55: 14000000 INFO @ Sat, 15 Jan 2022 20:47:58: 22000000 INFO @ Sat, 15 Jan 2022 20:48:01: 10000000 INFO @ Sat, 15 Jan 2022 20:48:01: 15000000 INFO @ Sat, 15 Jan 2022 20:48:03: 23000000 INFO @ Sat, 15 Jan 2022 20:48:07: 11000000 INFO @ Sat, 15 Jan 2022 20:48:08: 16000000 INFO @ Sat, 15 Jan 2022 20:48:09: 24000000 INFO @ Sat, 15 Jan 2022 20:48:13: 12000000 INFO @ Sat, 15 Jan 2022 20:48:14: 17000000 INFO @ Sat, 15 Jan 2022 20:48:15: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:48:19: 13000000 INFO @ Sat, 15 Jan 2022 20:48:20: 18000000 INFO @ Sat, 15 Jan 2022 20:48:21: 26000000 INFO @ Sat, 15 Jan 2022 20:48:26: 14000000 INFO @ Sat, 15 Jan 2022 20:48:26: 19000000 INFO @ Sat, 15 Jan 2022 20:48:26: 27000000 INFO @ Sat, 15 Jan 2022 20:48:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:48:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:48:27: #1 total tags in treatment: 8640374 INFO @ Sat, 15 Jan 2022 20:48:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:27: #1 tags after filtering in treatment: 6334157 INFO @ Sat, 15 Jan 2022 20:48:27: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 20:48:27: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:48:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:48:32: 15000000 INFO @ Sat, 15 Jan 2022 20:48:32: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:48:38: 16000000 INFO @ Sat, 15 Jan 2022 20:48:38: 21000000 INFO @ Sat, 15 Jan 2022 20:48:44: 17000000 INFO @ Sat, 15 Jan 2022 20:48:45: 22000000 INFO @ Sat, 15 Jan 2022 20:48:50: 18000000 INFO @ Sat, 15 Jan 2022 20:48:51: 23000000 INFO @ Sat, 15 Jan 2022 20:48:56: 19000000 INFO @ Sat, 15 Jan 2022 20:48:57: 24000000 INFO @ Sat, 15 Jan 2022 20:49:03: 20000000 INFO @ Sat, 15 Jan 2022 20:49:03: 25000000 INFO @ Sat, 15 Jan 2022 20:49:09: 21000000 INFO @ Sat, 15 Jan 2022 20:49:09: 26000000 INFO @ Sat, 15 Jan 2022 20:49:15: 22000000 INFO @ Sat, 15 Jan 2022 20:49:16: 27000000 INFO @ Sat, 15 Jan 2022 20:49:17: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:49:17: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:49:17: #1 total tags in treatment: 8640374 INFO @ Sat, 15 Jan 2022 20:49:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:49:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:49:17: #1 tags after filtering in treatment: 6334157 INFO @ Sat, 15 Jan 2022 20:49:17: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 20:49:17: #1 finished! INFO @ Sat, 15 Jan 2022 20:49:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:49:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:49:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:49:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:49:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:49:21: 23000000 INFO @ Sat, 15 Jan 2022 20:49:27: 24000000 INFO @ Sat, 15 Jan 2022 20:49:33: 25000000 INFO @ Sat, 15 Jan 2022 20:49:38: 26000000 INFO @ Sat, 15 Jan 2022 20:49:44: 27000000 INFO @ Sat, 15 Jan 2022 20:49:45: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:49:45: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:49:45: #1 total tags in treatment: 8640374 INFO @ Sat, 15 Jan 2022 20:49:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:49:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:49:45: #1 tags after filtering in treatment: 6334157 INFO @ Sat, 15 Jan 2022 20:49:45: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 20:49:45: #1 finished! INFO @ Sat, 15 Jan 2022 20:49:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:49:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:49:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:49:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:49:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245350/SRX8245350.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling