Job ID = 14521035 SRX = SRX8245349 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20285456 spots for SRR11684560/SRR11684560.sra Written 20285456 spots for SRR11684560/SRR11684560.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:13 20285456 reads; of these: 20285456 (100.00%) were paired; of these: 9758908 (48.11%) aligned concordantly 0 times 8984383 (44.29%) aligned concordantly exactly 1 time 1542165 (7.60%) aligned concordantly >1 times ---- 9758908 pairs aligned concordantly 0 times; of these: 73042 (0.75%) aligned discordantly 1 time ---- 9685866 pairs aligned 0 times concordantly or discordantly; of these: 19371732 mates make up the pairs; of these: 10319808 (53.27%) aligned 0 times 7668847 (39.59%) aligned exactly 1 time 1383077 (7.14%) aligned >1 times 74.56% overall alignment rate Time searching: 00:17:13 Overall time: 00:17:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 315185 / 10598326 = 0.0297 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:56:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:56:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:56:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:57:02: 1000000 INFO @ Sat, 15 Jan 2022 20:57:13: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:57:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:57:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:57:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:57:24: 3000000 INFO @ Sat, 15 Jan 2022 20:57:31: 1000000 INFO @ Sat, 15 Jan 2022 20:57:36: 4000000 INFO @ Sat, 15 Jan 2022 20:57:41: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:57:48: 5000000 INFO @ Sat, 15 Jan 2022 20:57:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:57:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:57:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:57:52: 3000000 INFO @ Sat, 15 Jan 2022 20:58:00: 6000000 INFO @ Sat, 15 Jan 2022 20:58:03: 1000000 INFO @ Sat, 15 Jan 2022 20:58:03: 4000000 INFO @ Sat, 15 Jan 2022 20:58:13: 7000000 INFO @ Sat, 15 Jan 2022 20:58:14: 5000000 INFO @ Sat, 15 Jan 2022 20:58:17: 2000000 INFO @ Sat, 15 Jan 2022 20:58:25: 6000000 INFO @ Sat, 15 Jan 2022 20:58:26: 8000000 INFO @ Sat, 15 Jan 2022 20:58:32: 3000000 INFO @ Sat, 15 Jan 2022 20:58:37: 7000000 INFO @ Sat, 15 Jan 2022 20:58:40: 9000000 INFO @ Sat, 15 Jan 2022 20:58:48: 4000000 INFO @ Sat, 15 Jan 2022 20:58:49: 8000000 INFO @ Sat, 15 Jan 2022 20:58:54: 10000000 INFO @ Sat, 15 Jan 2022 20:59:02: 9000000 INFO @ Sat, 15 Jan 2022 20:59:04: 5000000 INFO @ Sat, 15 Jan 2022 20:59:07: 11000000 INFO @ Sat, 15 Jan 2022 20:59:15: 10000000 INFO @ Sat, 15 Jan 2022 20:59:19: 6000000 INFO @ Sat, 15 Jan 2022 20:59:21: 12000000 INFO @ Sat, 15 Jan 2022 20:59:27: 11000000 INFO @ Sat, 15 Jan 2022 20:59:33: 13000000 INFO @ Sat, 15 Jan 2022 20:59:33: 7000000 INFO @ Sat, 15 Jan 2022 20:59:38: 12000000 INFO @ Sat, 15 Jan 2022 20:59:47: 14000000 INFO @ Sat, 15 Jan 2022 20:59:49: 8000000 INFO @ Sat, 15 Jan 2022 20:59:50: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:59:58: 15000000 INFO @ Sat, 15 Jan 2022 21:00:01: 14000000 INFO @ Sat, 15 Jan 2022 21:00:04: 9000000 INFO @ Sat, 15 Jan 2022 21:00:10: 16000000 INFO @ Sat, 15 Jan 2022 21:00:12: 15000000 INFO @ Sat, 15 Jan 2022 21:00:19: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:00:20: 17000000 INFO @ Sat, 15 Jan 2022 21:00:23: 16000000 INFO @ Sat, 15 Jan 2022 21:00:32: 18000000 INFO @ Sat, 15 Jan 2022 21:00:34: 11000000 INFO @ Sat, 15 Jan 2022 21:00:35: 17000000 INFO @ Sat, 15 Jan 2022 21:00:42: 19000000 INFO @ Sat, 15 Jan 2022 21:00:45: 18000000 INFO @ Sat, 15 Jan 2022 21:00:49: 12000000 INFO @ Sat, 15 Jan 2022 21:00:52: 20000000 INFO @ Sat, 15 Jan 2022 21:00:56: 19000000 INFO @ Sat, 15 Jan 2022 21:01:02: 21000000 INFO @ Sat, 15 Jan 2022 21:01:03: 13000000 INFO @ Sat, 15 Jan 2022 21:01:07: 20000000 INFO @ Sat, 15 Jan 2022 21:01:13: 22000000 INFO @ Sat, 15 Jan 2022 21:01:18: 21000000 INFO @ Sat, 15 Jan 2022 21:01:18: 14000000 INFO @ Sat, 15 Jan 2022 21:01:25: 23000000 INFO @ Sat, 15 Jan 2022 21:01:29: 22000000 INFO @ Sat, 15 Jan 2022 21:01:32: 15000000 INFO @ Sat, 15 Jan 2022 21:01:36: 24000000 INFO @ Sat, 15 Jan 2022 21:01:39: 23000000 INFO @ Sat, 15 Jan 2022 21:01:46: 16000000 INFO @ Sat, 15 Jan 2022 21:01:47: 25000000 INFO @ Sat, 15 Jan 2022 21:01:50: 24000000 INFO @ Sat, 15 Jan 2022 21:01:58: 26000000 INFO @ Sat, 15 Jan 2022 21:02:01: 25000000 INFO @ Sat, 15 Jan 2022 21:02:01: 17000000 INFO @ Sat, 15 Jan 2022 21:02:08: 27000000 INFO @ Sat, 15 Jan 2022 21:02:11: 26000000 INFO @ Sat, 15 Jan 2022 21:02:15: 18000000 INFO @ Sat, 15 Jan 2022 21:02:18: 28000000 INFO @ Sat, 15 Jan 2022 21:02:23: 27000000 INFO @ Sat, 15 Jan 2022 21:02:28: 29000000 INFO @ Sat, 15 Jan 2022 21:02:30: 19000000 INFO @ Sat, 15 Jan 2022 21:02:35: 28000000 INFO @ Sat, 15 Jan 2022 21:02:35: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:02:35: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:02:35: #1 total tags in treatment: 10211565 INFO @ Sat, 15 Jan 2022 21:02:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:02:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:02:35: #1 tags after filtering in treatment: 7170030 INFO @ Sat, 15 Jan 2022 21:02:35: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:02:35: #1 finished! INFO @ Sat, 15 Jan 2022 21:02:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:02:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:02:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:02:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:02:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:02:44: 20000000 INFO @ Sat, 15 Jan 2022 21:02:46: 29000000 INFO @ Sat, 15 Jan 2022 21:02:53: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:02:53: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:02:53: #1 total tags in treatment: 10211565 INFO @ Sat, 15 Jan 2022 21:02:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:02:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:02:53: #1 tags after filtering in treatment: 7170030 INFO @ Sat, 15 Jan 2022 21:02:53: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:02:53: #1 finished! INFO @ Sat, 15 Jan 2022 21:02:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:02:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:02:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:02:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:02:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:02:59: 21000000 INFO @ Sat, 15 Jan 2022 21:03:14: 22000000 INFO @ Sat, 15 Jan 2022 21:03:29: 23000000 INFO @ Sat, 15 Jan 2022 21:03:43: 24000000 INFO @ Sat, 15 Jan 2022 21:03:57: 25000000 INFO @ Sat, 15 Jan 2022 21:04:11: 26000000 INFO @ Sat, 15 Jan 2022 21:04:24: 27000000 INFO @ Sat, 15 Jan 2022 21:04:38: 28000000 INFO @ Sat, 15 Jan 2022 21:04:52: 29000000 INFO @ Sat, 15 Jan 2022 21:05:00: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:05:00: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:05:00: #1 total tags in treatment: 10211565 INFO @ Sat, 15 Jan 2022 21:05:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:05:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:05:00: #1 tags after filtering in treatment: 7170030 INFO @ Sat, 15 Jan 2022 21:05:00: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:05:00: #1 finished! INFO @ Sat, 15 Jan 2022 21:05:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:05:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:05:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:05:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:05:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245349/SRX8245349.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling