Job ID = 14521034 SRX = SRX8245348 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13103213 spots for SRR11684559/SRR11684559.sra Written 13103213 spots for SRR11684559/SRR11684559.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:11 13103213 reads; of these: 13103213 (100.00%) were paired; of these: 6624107 (50.55%) aligned concordantly 0 times 5550481 (42.36%) aligned concordantly exactly 1 time 928625 (7.09%) aligned concordantly >1 times ---- 6624107 pairs aligned concordantly 0 times; of these: 42596 (0.64%) aligned discordantly 1 time ---- 6581511 pairs aligned 0 times concordantly or discordantly; of these: 13163022 mates make up the pairs; of these: 7009492 (53.25%) aligned 0 times 5235836 (39.78%) aligned exactly 1 time 917694 (6.97%) aligned >1 times 73.25% overall alignment rate Time searching: 00:08:11 Overall time: 00:08:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 134522 / 6520934 = 0.0206 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:36:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:36:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:36:21: 1000000 INFO @ Sat, 15 Jan 2022 20:36:25: 2000000 INFO @ Sat, 15 Jan 2022 20:36:30: 3000000 INFO @ Sat, 15 Jan 2022 20:36:35: 4000000 INFO @ Sat, 15 Jan 2022 20:36:40: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:36:45: 6000000 INFO @ Sat, 15 Jan 2022 20:36:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:36:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:36:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:36:51: 7000000 INFO @ Sat, 15 Jan 2022 20:36:53: 1000000 INFO @ Sat, 15 Jan 2022 20:36:57: 8000000 INFO @ Sat, 15 Jan 2022 20:36:59: 2000000 INFO @ Sat, 15 Jan 2022 20:37:03: 9000000 INFO @ Sat, 15 Jan 2022 20:37:06: 3000000 INFO @ Sat, 15 Jan 2022 20:37:09: 10000000 INFO @ Sat, 15 Jan 2022 20:37:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:37:15: 11000000 INFO @ Sat, 15 Jan 2022 20:37:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:37:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:37:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:37:20: 5000000 INFO @ Sat, 15 Jan 2022 20:37:21: 12000000 INFO @ Sat, 15 Jan 2022 20:37:23: 1000000 INFO @ Sat, 15 Jan 2022 20:37:27: 6000000 INFO @ Sat, 15 Jan 2022 20:37:27: 13000000 INFO @ Sat, 15 Jan 2022 20:37:30: 2000000 INFO @ Sat, 15 Jan 2022 20:37:33: 14000000 INFO @ Sat, 15 Jan 2022 20:37:34: 7000000 INFO @ Sat, 15 Jan 2022 20:37:37: 3000000 INFO @ Sat, 15 Jan 2022 20:37:39: 15000000 INFO @ Sat, 15 Jan 2022 20:37:41: 8000000 INFO @ Sat, 15 Jan 2022 20:37:44: 4000000 INFO @ Sat, 15 Jan 2022 20:37:45: 16000000 INFO @ Sat, 15 Jan 2022 20:37:48: 9000000 INFO @ Sat, 15 Jan 2022 20:37:50: 5000000 INFO @ Sat, 15 Jan 2022 20:37:51: 17000000 INFO @ Sat, 15 Jan 2022 20:37:55: 10000000 INFO @ Sat, 15 Jan 2022 20:37:57: 18000000 INFO @ Sat, 15 Jan 2022 20:37:57: 6000000 INFO @ Sat, 15 Jan 2022 20:38:02: 11000000 INFO @ Sat, 15 Jan 2022 20:38:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:38:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:38:03: #1 total tags in treatment: 6344671 INFO @ Sat, 15 Jan 2022 20:38:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:38:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:38:03: #1 tags after filtering in treatment: 4906608 INFO @ Sat, 15 Jan 2022 20:38:03: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:38:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:38:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:38:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:38:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:38:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:38:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:38:05: 7000000 INFO @ Sat, 15 Jan 2022 20:38:09: 12000000 INFO @ Sat, 15 Jan 2022 20:38:12: 8000000 INFO @ Sat, 15 Jan 2022 20:38:15: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:38:19: 9000000 INFO @ Sat, 15 Jan 2022 20:38:22: 14000000 INFO @ Sat, 15 Jan 2022 20:38:26: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:38:29: 15000000 INFO @ Sat, 15 Jan 2022 20:38:33: 11000000 INFO @ Sat, 15 Jan 2022 20:38:36: 16000000 INFO @ Sat, 15 Jan 2022 20:38:40: 12000000 INFO @ Sat, 15 Jan 2022 20:38:43: 17000000 INFO @ Sat, 15 Jan 2022 20:38:47: 13000000 INFO @ Sat, 15 Jan 2022 20:38:50: 18000000 INFO @ Sat, 15 Jan 2022 20:38:54: 14000000 INFO @ Sat, 15 Jan 2022 20:38:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:38:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:38:56: #1 total tags in treatment: 6344671 INFO @ Sat, 15 Jan 2022 20:38:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:38:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:38:56: #1 tags after filtering in treatment: 4906608 INFO @ Sat, 15 Jan 2022 20:38:56: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:38:56: #1 finished! INFO @ Sat, 15 Jan 2022 20:38:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:38:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:38:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:38:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:38:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:39:00: 15000000 INFO @ Sat, 15 Jan 2022 20:39:07: 16000000 INFO @ Sat, 15 Jan 2022 20:39:13: 17000000 INFO @ Sat, 15 Jan 2022 20:39:20: 18000000 INFO @ Sat, 15 Jan 2022 20:39:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:39:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:39:25: #1 total tags in treatment: 6344671 INFO @ Sat, 15 Jan 2022 20:39:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:39:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:39:26: #1 tags after filtering in treatment: 4906608 INFO @ Sat, 15 Jan 2022 20:39:26: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:39:26: #1 finished! INFO @ Sat, 15 Jan 2022 20:39:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:39:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:39:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:39:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:39:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245348/SRX8245348.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling