Job ID = 14521033 SRX = SRX8245347 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9976811 spots for SRR11684558/SRR11684558.sra Written 9976811 spots for SRR11684558/SRR11684558.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:59 9976811 reads; of these: 9976811 (100.00%) were paired; of these: 4329295 (43.39%) aligned concordantly 0 times 4891390 (49.03%) aligned concordantly exactly 1 time 756126 (7.58%) aligned concordantly >1 times ---- 4329295 pairs aligned concordantly 0 times; of these: 24903 (0.58%) aligned discordantly 1 time ---- 4304392 pairs aligned 0 times concordantly or discordantly; of these: 8608784 mates make up the pairs; of these: 4714143 (54.76%) aligned 0 times 3347948 (38.89%) aligned exactly 1 time 546693 (6.35%) aligned >1 times 76.37% overall alignment rate Time searching: 00:05:59 Overall time: 00:05:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 87933 / 5671652 = 0.0155 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:14: 1000000 INFO @ Sat, 15 Jan 2022 20:31:20: 2000000 INFO @ Sat, 15 Jan 2022 20:31:26: 3000000 INFO @ Sat, 15 Jan 2022 20:31:32: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:37: 5000000 INFO @ Sat, 15 Jan 2022 20:31:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:44: 6000000 INFO @ Sat, 15 Jan 2022 20:31:44: 1000000 INFO @ Sat, 15 Jan 2022 20:31:50: 7000000 INFO @ Sat, 15 Jan 2022 20:31:51: 2000000 INFO @ Sat, 15 Jan 2022 20:31:57: 8000000 INFO @ Sat, 15 Jan 2022 20:31:57: 3000000 INFO @ Sat, 15 Jan 2022 20:32:03: 9000000 INFO @ Sat, 15 Jan 2022 20:32:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:32:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:32:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:32:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:32:10: 10000000 INFO @ Sat, 15 Jan 2022 20:32:11: 5000000 INFO @ Sat, 15 Jan 2022 20:32:14: 1000000 INFO @ Sat, 15 Jan 2022 20:32:16: 11000000 INFO @ Sat, 15 Jan 2022 20:32:17: 6000000 INFO @ Sat, 15 Jan 2022 20:32:19: 2000000 INFO @ Sat, 15 Jan 2022 20:32:23: 12000000 INFO @ Sat, 15 Jan 2022 20:32:24: 7000000 INFO @ Sat, 15 Jan 2022 20:32:25: 3000000 INFO @ Sat, 15 Jan 2022 20:32:29: 13000000 INFO @ Sat, 15 Jan 2022 20:32:30: 8000000 INFO @ Sat, 15 Jan 2022 20:32:31: 4000000 INFO @ Sat, 15 Jan 2022 20:32:36: 14000000 INFO @ Sat, 15 Jan 2022 20:32:36: 5000000 INFO @ Sat, 15 Jan 2022 20:32:37: 9000000 INFO @ Sat, 15 Jan 2022 20:32:42: 6000000 INFO @ Sat, 15 Jan 2022 20:32:43: 15000000 INFO @ Sat, 15 Jan 2022 20:32:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:43: #1 total tags in treatment: 5559628 INFO @ Sat, 15 Jan 2022 20:32:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:43: #1 tags after filtering in treatment: 4465233 INFO @ Sat, 15 Jan 2022 20:32:43: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:32:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:43: #2 number of paired peaks: 24 WARNING @ Sat, 15 Jan 2022 20:32:43: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:32:44: 10000000 INFO @ Sat, 15 Jan 2022 20:32:48: 7000000 INFO @ Sat, 15 Jan 2022 20:32:50: 11000000 INFO @ Sat, 15 Jan 2022 20:32:53: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:32:57: 12000000 INFO @ Sat, 15 Jan 2022 20:32:59: 9000000 INFO @ Sat, 15 Jan 2022 20:33:04: 13000000 INFO @ Sat, 15 Jan 2022 20:33:04: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:33:10: 11000000 INFO @ Sat, 15 Jan 2022 20:33:10: 14000000 INFO @ Sat, 15 Jan 2022 20:33:16: 12000000 INFO @ Sat, 15 Jan 2022 20:33:17: 15000000 INFO @ Sat, 15 Jan 2022 20:33:17: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:33:17: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:33:17: #1 total tags in treatment: 5559628 INFO @ Sat, 15 Jan 2022 20:33:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:33:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:33:17: #1 tags after filtering in treatment: 4465233 INFO @ Sat, 15 Jan 2022 20:33:17: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:33:17: #1 finished! INFO @ Sat, 15 Jan 2022 20:33:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:33:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:33:18: #2 number of paired peaks: 24 WARNING @ Sat, 15 Jan 2022 20:33:18: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:33:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:33:21: 13000000 INFO @ Sat, 15 Jan 2022 20:33:26: 14000000 INFO @ Sat, 15 Jan 2022 20:33:31: 15000000 INFO @ Sat, 15 Jan 2022 20:33:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:33:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:33:31: #1 total tags in treatment: 5559628 INFO @ Sat, 15 Jan 2022 20:33:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:33:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:33:31: #1 tags after filtering in treatment: 4465233 INFO @ Sat, 15 Jan 2022 20:33:31: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:33:31: #1 finished! INFO @ Sat, 15 Jan 2022 20:33:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:33:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:33:31: #2 number of paired peaks: 24 WARNING @ Sat, 15 Jan 2022 20:33:31: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:33:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245347/SRX8245347.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling