Job ID = 14521032 SRX = SRX8245346 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10281617 spots for SRR11684557/SRR11684557.sra Written 10281617 spots for SRR11684557/SRR11684557.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:43 10281617 reads; of these: 10281617 (100.00%) were paired; of these: 5978909 (58.15%) aligned concordantly 0 times 3766711 (36.64%) aligned concordantly exactly 1 time 535997 (5.21%) aligned concordantly >1 times ---- 5978909 pairs aligned concordantly 0 times; of these: 21883 (0.37%) aligned discordantly 1 time ---- 5957026 pairs aligned 0 times concordantly or discordantly; of these: 11914052 mates make up the pairs; of these: 6437500 (54.03%) aligned 0 times 4756917 (39.93%) aligned exactly 1 time 719635 (6.04%) aligned >1 times 68.69% overall alignment rate Time searching: 00:05:43 Overall time: 00:05:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 48897 / 4324012 = 0.0113 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:30:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:30:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:30:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:30:52: 1000000 INFO @ Sat, 15 Jan 2022 20:30:56: 2000000 INFO @ Sat, 15 Jan 2022 20:31:01: 3000000 INFO @ Sat, 15 Jan 2022 20:31:05: 4000000 INFO @ Sat, 15 Jan 2022 20:31:10: 5000000 INFO @ Sat, 15 Jan 2022 20:31:14: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:19: 7000000 INFO @ Sat, 15 Jan 2022 20:31:22: 1000000 INFO @ Sat, 15 Jan 2022 20:31:24: 8000000 INFO @ Sat, 15 Jan 2022 20:31:27: 2000000 INFO @ Sat, 15 Jan 2022 20:31:29: 9000000 INFO @ Sat, 15 Jan 2022 20:31:32: 3000000 INFO @ Sat, 15 Jan 2022 20:31:34: 10000000 INFO @ Sat, 15 Jan 2022 20:31:37: 4000000 INFO @ Sat, 15 Jan 2022 20:31:39: 11000000 INFO @ Sat, 15 Jan 2022 20:31:43: 5000000 INFO @ Sat, 15 Jan 2022 20:31:44: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:48: 6000000 INFO @ Sat, 15 Jan 2022 20:31:50: 13000000 INFO @ Sat, 15 Jan 2022 20:31:53: 1000000 INFO @ Sat, 15 Jan 2022 20:31:53: 7000000 INFO @ Sat, 15 Jan 2022 20:31:55: 14000000 INFO @ Sat, 15 Jan 2022 20:31:55: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:31:55: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:31:55: #1 total tags in treatment: 4253841 INFO @ Sat, 15 Jan 2022 20:31:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:31:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:31:55: #1 tags after filtering in treatment: 3549584 INFO @ Sat, 15 Jan 2022 20:31:55: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:31:55: #1 finished! INFO @ Sat, 15 Jan 2022 20:31:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:31:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:31:55: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 20:31:55: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:31:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:31:59: 8000000 INFO @ Sat, 15 Jan 2022 20:31:59: 2000000 INFO @ Sat, 15 Jan 2022 20:32:04: 9000000 INFO @ Sat, 15 Jan 2022 20:32:05: 3000000 INFO @ Sat, 15 Jan 2022 20:32:09: 10000000 INFO @ Sat, 15 Jan 2022 20:32:10: 4000000 INFO @ Sat, 15 Jan 2022 20:32:15: 11000000 INFO @ Sat, 15 Jan 2022 20:32:16: 5000000 INFO @ Sat, 15 Jan 2022 20:32:20: 12000000 INFO @ Sat, 15 Jan 2022 20:32:23: 6000000 INFO @ Sat, 15 Jan 2022 20:32:26: 13000000 INFO @ Sat, 15 Jan 2022 20:32:29: 7000000 INFO @ Sat, 15 Jan 2022 20:32:31: 14000000 INFO @ Sat, 15 Jan 2022 20:32:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:31: #1 total tags in treatment: 4253841 INFO @ Sat, 15 Jan 2022 20:32:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:32: #1 tags after filtering in treatment: 3549584 INFO @ Sat, 15 Jan 2022 20:32:32: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:32:32: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:32: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 20:32:32: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:32: Process for pairing-model is terminated! BedGraph に変換しました。 BigWig に変換中... cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:32:34: 8000000 INFO @ Sat, 15 Jan 2022 20:32:40: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:32:46: 10000000 INFO @ Sat, 15 Jan 2022 20:32:52: 11000000 INFO @ Sat, 15 Jan 2022 20:32:57: 12000000 INFO @ Sat, 15 Jan 2022 20:33:03: 13000000 INFO @ Sat, 15 Jan 2022 20:33:08: 14000000 INFO @ Sat, 15 Jan 2022 20:33:09: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:33:09: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:33:09: #1 total tags in treatment: 4253841 INFO @ Sat, 15 Jan 2022 20:33:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:33:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:33:09: #1 tags after filtering in treatment: 3549584 INFO @ Sat, 15 Jan 2022 20:33:09: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:33:09: #1 finished! INFO @ Sat, 15 Jan 2022 20:33:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:33:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:33:09: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 20:33:09: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:33:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245346/SRX8245346.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling