Job ID = 14521029 SRX = SRX8245343 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 29273196 spots for SRR11684554/SRR11684554.sra Written 29273196 spots for SRR11684554/SRR11684554.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:32 29273196 reads; of these: 29273196 (100.00%) were paired; of these: 13874014 (47.39%) aligned concordantly 0 times 13256058 (45.28%) aligned concordantly exactly 1 time 2143124 (7.32%) aligned concordantly >1 times ---- 13874014 pairs aligned concordantly 0 times; of these: 403367 (2.91%) aligned discordantly 1 time ---- 13470647 pairs aligned 0 times concordantly or discordantly; of these: 26941294 mates make up the pairs; of these: 14677275 (54.48%) aligned 0 times 10357769 (38.45%) aligned exactly 1 time 1906250 (7.08%) aligned >1 times 74.93% overall alignment rate Time searching: 00:16:32 Overall time: 00:16:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 519490 / 15800821 = 0.0329 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:50:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:50:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:50:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:50:38: 1000000 INFO @ Sat, 15 Jan 2022 20:50:44: 2000000 INFO @ Sat, 15 Jan 2022 20:50:49: 3000000 INFO @ Sat, 15 Jan 2022 20:50:54: 4000000 INFO @ Sat, 15 Jan 2022 20:51:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:51:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:51:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:51:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:51:07: 6000000 INFO @ Sat, 15 Jan 2022 20:51:10: 1000000 INFO @ Sat, 15 Jan 2022 20:51:14: 7000000 INFO @ Sat, 15 Jan 2022 20:51:18: 2000000 INFO @ Sat, 15 Jan 2022 20:51:21: 8000000 INFO @ Sat, 15 Jan 2022 20:51:25: 3000000 INFO @ Sat, 15 Jan 2022 20:51:28: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:51:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:51:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:51:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:51:33: 4000000 INFO @ Sat, 15 Jan 2022 20:51:35: 10000000 INFO @ Sat, 15 Jan 2022 20:51:40: 1000000 INFO @ Sat, 15 Jan 2022 20:51:40: 5000000 INFO @ Sat, 15 Jan 2022 20:51:42: 11000000 INFO @ Sat, 15 Jan 2022 20:51:47: 2000000 INFO @ Sat, 15 Jan 2022 20:51:48: 6000000 INFO @ Sat, 15 Jan 2022 20:51:49: 12000000 INFO @ Sat, 15 Jan 2022 20:51:54: 3000000 INFO @ Sat, 15 Jan 2022 20:51:56: 7000000 INFO @ Sat, 15 Jan 2022 20:51:56: 13000000 INFO @ Sat, 15 Jan 2022 20:52:01: 4000000 INFO @ Sat, 15 Jan 2022 20:52:03: 14000000 INFO @ Sat, 15 Jan 2022 20:52:04: 8000000 INFO @ Sat, 15 Jan 2022 20:52:08: 5000000 INFO @ Sat, 15 Jan 2022 20:52:10: 15000000 INFO @ Sat, 15 Jan 2022 20:52:11: 9000000 INFO @ Sat, 15 Jan 2022 20:52:15: 6000000 INFO @ Sat, 15 Jan 2022 20:52:17: 16000000 INFO @ Sat, 15 Jan 2022 20:52:19: 10000000 INFO @ Sat, 15 Jan 2022 20:52:22: 7000000 INFO @ Sat, 15 Jan 2022 20:52:24: 17000000 INFO @ Sat, 15 Jan 2022 20:52:27: 11000000 INFO @ Sat, 15 Jan 2022 20:52:29: 8000000 INFO @ Sat, 15 Jan 2022 20:52:32: 18000000 INFO @ Sat, 15 Jan 2022 20:52:34: 12000000 INFO @ Sat, 15 Jan 2022 20:52:36: 9000000 INFO @ Sat, 15 Jan 2022 20:52:39: 19000000 INFO @ Sat, 15 Jan 2022 20:52:42: 13000000 INFO @ Sat, 15 Jan 2022 20:52:43: 10000000 INFO @ Sat, 15 Jan 2022 20:52:46: 20000000 INFO @ Sat, 15 Jan 2022 20:52:50: 14000000 INFO @ Sat, 15 Jan 2022 20:52:50: 11000000 INFO @ Sat, 15 Jan 2022 20:52:53: 21000000 INFO @ Sat, 15 Jan 2022 20:52:58: 12000000 INFO @ Sat, 15 Jan 2022 20:52:58: 15000000 INFO @ Sat, 15 Jan 2022 20:53:00: 22000000 INFO @ Sat, 15 Jan 2022 20:53:05: 13000000 INFO @ Sat, 15 Jan 2022 20:53:05: 16000000 INFO @ Sat, 15 Jan 2022 20:53:08: 23000000 INFO @ Sat, 15 Jan 2022 20:53:12: 14000000 INFO @ Sat, 15 Jan 2022 20:53:13: 17000000 INFO @ Sat, 15 Jan 2022 20:53:15: 24000000 INFO @ Sat, 15 Jan 2022 20:53:19: 15000000 INFO @ Sat, 15 Jan 2022 20:53:20: 18000000 INFO @ Sat, 15 Jan 2022 20:53:22: 25000000 INFO @ Sat, 15 Jan 2022 20:53:26: 16000000 INFO @ Sat, 15 Jan 2022 20:53:28: 19000000 INFO @ Sat, 15 Jan 2022 20:53:29: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:53:34: 17000000 INFO @ Sat, 15 Jan 2022 20:53:36: 20000000 INFO @ Sat, 15 Jan 2022 20:53:36: 27000000 INFO @ Sat, 15 Jan 2022 20:53:41: 18000000 INFO @ Sat, 15 Jan 2022 20:53:43: 21000000 INFO @ Sat, 15 Jan 2022 20:53:43: 28000000 INFO @ Sat, 15 Jan 2022 20:53:48: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:53:50: 29000000 INFO @ Sat, 15 Jan 2022 20:53:51: 22000000 INFO @ Sat, 15 Jan 2022 20:53:55: 20000000 INFO @ Sat, 15 Jan 2022 20:53:57: 30000000 INFO @ Sat, 15 Jan 2022 20:53:59: 23000000 INFO @ Sat, 15 Jan 2022 20:54:02: 21000000 INFO @ Sat, 15 Jan 2022 20:54:04: 31000000 INFO @ Sat, 15 Jan 2022 20:54:06: 24000000 INFO @ Sat, 15 Jan 2022 20:54:09: 22000000 INFO @ Sat, 15 Jan 2022 20:54:11: 32000000 INFO @ Sat, 15 Jan 2022 20:54:14: 25000000 INFO @ Sat, 15 Jan 2022 20:54:16: 23000000 INFO @ Sat, 15 Jan 2022 20:54:18: 33000000 INFO @ Sat, 15 Jan 2022 20:54:22: 26000000 INFO @ Sat, 15 Jan 2022 20:54:23: 24000000 INFO @ Sat, 15 Jan 2022 20:54:25: 34000000 INFO @ Sat, 15 Jan 2022 20:54:29: 27000000 INFO @ Sat, 15 Jan 2022 20:54:30: 25000000 INFO @ Sat, 15 Jan 2022 20:54:32: 35000000 INFO @ Sat, 15 Jan 2022 20:54:37: 26000000 INFO @ Sat, 15 Jan 2022 20:54:37: 28000000 INFO @ Sat, 15 Jan 2022 20:54:39: 36000000 INFO @ Sat, 15 Jan 2022 20:54:44: 27000000 INFO @ Sat, 15 Jan 2022 20:54:45: 29000000 INFO @ Sat, 15 Jan 2022 20:54:46: 37000000 INFO @ Sat, 15 Jan 2022 20:54:51: 28000000 INFO @ Sat, 15 Jan 2022 20:54:52: 30000000 INFO @ Sat, 15 Jan 2022 20:54:53: 38000000 INFO @ Sat, 15 Jan 2022 20:54:58: 29000000 INFO @ Sat, 15 Jan 2022 20:55:00: 31000000 INFO @ Sat, 15 Jan 2022 20:55:00: 39000000 INFO @ Sat, 15 Jan 2022 20:55:05: 30000000 INFO @ Sat, 15 Jan 2022 20:55:07: 40000000 INFO @ Sat, 15 Jan 2022 20:55:08: 32000000 INFO @ Sat, 15 Jan 2022 20:55:12: 31000000 INFO @ Sat, 15 Jan 2022 20:55:14: 41000000 INFO @ Sat, 15 Jan 2022 20:55:16: 33000000 INFO @ Sat, 15 Jan 2022 20:55:19: 32000000 INFO @ Sat, 15 Jan 2022 20:55:21: 42000000 INFO @ Sat, 15 Jan 2022 20:55:23: 34000000 INFO @ Sat, 15 Jan 2022 20:55:26: 33000000 INFO @ Sat, 15 Jan 2022 20:55:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:55:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:55:27: #1 total tags in treatment: 14883286 INFO @ Sat, 15 Jan 2022 20:55:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:55:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:55:27: #1 tags after filtering in treatment: 9791550 INFO @ Sat, 15 Jan 2022 20:55:27: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 20:55:27: #1 finished! INFO @ Sat, 15 Jan 2022 20:55:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:55:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:55:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:55:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:55:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:55:31: 35000000 INFO @ Sat, 15 Jan 2022 20:55:33: 34000000 INFO @ Sat, 15 Jan 2022 20:55:39: 36000000 INFO @ Sat, 15 Jan 2022 20:55:40: 35000000 INFO @ Sat, 15 Jan 2022 20:55:46: 37000000 INFO @ Sat, 15 Jan 2022 20:55:47: 36000000 INFO @ Sat, 15 Jan 2022 20:55:54: 37000000 INFO @ Sat, 15 Jan 2022 20:55:54: 38000000 INFO @ Sat, 15 Jan 2022 20:56:00: 38000000 INFO @ Sat, 15 Jan 2022 20:56:02: 39000000 INFO @ Sat, 15 Jan 2022 20:56:07: 39000000 INFO @ Sat, 15 Jan 2022 20:56:10: 40000000 INFO @ Sat, 15 Jan 2022 20:56:14: 40000000 INFO @ Sat, 15 Jan 2022 20:56:17: 41000000 INFO @ Sat, 15 Jan 2022 20:56:21: 41000000 INFO @ Sat, 15 Jan 2022 20:56:25: 42000000 INFO @ Sat, 15 Jan 2022 20:56:28: 42000000 INFO @ Sat, 15 Jan 2022 20:56:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:56:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:56:31: #1 total tags in treatment: 14883286 INFO @ Sat, 15 Jan 2022 20:56:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:56:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:56:32: #1 tags after filtering in treatment: 9791550 INFO @ Sat, 15 Jan 2022 20:56:32: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 20:56:32: #1 finished! INFO @ Sat, 15 Jan 2022 20:56:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:56:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:56:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:56:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:56:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:56:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:56:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:56:34: #1 total tags in treatment: 14883286 INFO @ Sat, 15 Jan 2022 20:56:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:56:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:56:34: #1 tags after filtering in treatment: 9791550 INFO @ Sat, 15 Jan 2022 20:56:34: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 20:56:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:56:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:56:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:56:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:56:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:56:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245343/SRX8245343.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling