Job ID = 14521028 SRX = SRX8245342 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11998280 spots for SRR11684553/SRR11684553.sra Written 11998280 spots for SRR11684553/SRR11684553.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:28 11998280 reads; of these: 11998280 (100.00%) were paired; of these: 4406676 (36.73%) aligned concordantly 0 times 6635809 (55.31%) aligned concordantly exactly 1 time 955795 (7.97%) aligned concordantly >1 times ---- 4406676 pairs aligned concordantly 0 times; of these: 41993 (0.95%) aligned discordantly 1 time ---- 4364683 pairs aligned 0 times concordantly or discordantly; of these: 8729366 mates make up the pairs; of these: 5013561 (57.43%) aligned 0 times 3201383 (36.67%) aligned exactly 1 time 514422 (5.89%) aligned >1 times 79.11% overall alignment rate Time searching: 00:10:28 Overall time: 00:10:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 199069 / 7632872 = 0.0261 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:40:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:40:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:40:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:41:04: 1000000 INFO @ Sat, 15 Jan 2022 20:41:12: 2000000 INFO @ Sat, 15 Jan 2022 20:41:20: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:41:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:41:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:41:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:41:29: 4000000 INFO @ Sat, 15 Jan 2022 20:41:34: 1000000 INFO @ Sat, 15 Jan 2022 20:41:37: 5000000 INFO @ Sat, 15 Jan 2022 20:41:43: 2000000 INFO @ Sat, 15 Jan 2022 20:41:46: 6000000 INFO @ Sat, 15 Jan 2022 20:41:52: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:41:55: 7000000 INFO @ Sat, 15 Jan 2022 20:41:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:41:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:41:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:42:01: 4000000 INFO @ Sat, 15 Jan 2022 20:42:04: 8000000 INFO @ Sat, 15 Jan 2022 20:42:05: 1000000 INFO @ Sat, 15 Jan 2022 20:42:11: 5000000 INFO @ Sat, 15 Jan 2022 20:42:13: 9000000 INFO @ Sat, 15 Jan 2022 20:42:14: 2000000 INFO @ Sat, 15 Jan 2022 20:42:21: 6000000 INFO @ Sat, 15 Jan 2022 20:42:22: 10000000 INFO @ Sat, 15 Jan 2022 20:42:23: 3000000 INFO @ Sat, 15 Jan 2022 20:42:30: 7000000 INFO @ Sat, 15 Jan 2022 20:42:31: 4000000 INFO @ Sat, 15 Jan 2022 20:42:31: 11000000 INFO @ Sat, 15 Jan 2022 20:42:38: 8000000 INFO @ Sat, 15 Jan 2022 20:42:39: 5000000 INFO @ Sat, 15 Jan 2022 20:42:40: 12000000 INFO @ Sat, 15 Jan 2022 20:42:47: 6000000 INFO @ Sat, 15 Jan 2022 20:42:47: 9000000 INFO @ Sat, 15 Jan 2022 20:42:49: 13000000 INFO @ Sat, 15 Jan 2022 20:42:55: 7000000 INFO @ Sat, 15 Jan 2022 20:42:57: 10000000 INFO @ Sat, 15 Jan 2022 20:42:57: 14000000 INFO @ Sat, 15 Jan 2022 20:43:03: 8000000 INFO @ Sat, 15 Jan 2022 20:43:06: 15000000 INFO @ Sat, 15 Jan 2022 20:43:06: 11000000 INFO @ Sat, 15 Jan 2022 20:43:11: 9000000 INFO @ Sat, 15 Jan 2022 20:43:15: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:43:16: 12000000 INFO @ Sat, 15 Jan 2022 20:43:18: 10000000 INFO @ Sat, 15 Jan 2022 20:43:24: 17000000 INFO @ Sat, 15 Jan 2022 20:43:25: 13000000 INFO @ Sat, 15 Jan 2022 20:43:26: 11000000 INFO @ Sat, 15 Jan 2022 20:43:33: 18000000 INFO @ Sat, 15 Jan 2022 20:43:33: 14000000 INFO @ Sat, 15 Jan 2022 20:43:34: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:43:38: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:43:38: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:43:38: #1 total tags in treatment: 7392634 INFO @ Sat, 15 Jan 2022 20:43:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:43:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:43:38: #1 tags after filtering in treatment: 5757674 INFO @ Sat, 15 Jan 2022 20:43:38: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 20:43:38: #1 finished! INFO @ Sat, 15 Jan 2022 20:43:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:43:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:43:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:43:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:43:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:43:42: 13000000 INFO @ Sat, 15 Jan 2022 20:43:42: 15000000 INFO @ Sat, 15 Jan 2022 20:43:49: 14000000 INFO @ Sat, 15 Jan 2022 20:43:51: 16000000 INFO @ Sat, 15 Jan 2022 20:43:57: 15000000 INFO @ Sat, 15 Jan 2022 20:44:00: 17000000 INFO @ Sat, 15 Jan 2022 20:44:05: 16000000 INFO @ Sat, 15 Jan 2022 20:44:09: 18000000 INFO @ Sat, 15 Jan 2022 20:44:12: 17000000 INFO @ Sat, 15 Jan 2022 20:44:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:44:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:44:14: #1 total tags in treatment: 7392634 INFO @ Sat, 15 Jan 2022 20:44:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:44:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:44:14: #1 tags after filtering in treatment: 5757674 INFO @ Sat, 15 Jan 2022 20:44:14: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 20:44:14: #1 finished! INFO @ Sat, 15 Jan 2022 20:44:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:44:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:44:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:44:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:44:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:44:19: 18000000 INFO @ Sat, 15 Jan 2022 20:44:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:44:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:44:24: #1 total tags in treatment: 7392634 INFO @ Sat, 15 Jan 2022 20:44:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:44:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:44:24: #1 tags after filtering in treatment: 5757674 INFO @ Sat, 15 Jan 2022 20:44:24: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 20:44:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:44:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:44:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:44:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:44:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:44:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245342/SRX8245342.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling