Job ID = 14521026 SRX = SRX8245340 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21631246 spots for SRR11684551/SRR11684551.sra Written 21631246 spots for SRR11684551/SRR11684551.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:59 21631246 reads; of these: 21631246 (100.00%) were paired; of these: 10865677 (50.23%) aligned concordantly 0 times 9363696 (43.29%) aligned concordantly exactly 1 time 1401873 (6.48%) aligned concordantly >1 times ---- 10865677 pairs aligned concordantly 0 times; of these: 409398 (3.77%) aligned discordantly 1 time ---- 10456279 pairs aligned 0 times concordantly or discordantly; of these: 20912558 mates make up the pairs; of these: 11441826 (54.71%) aligned 0 times 8055519 (38.52%) aligned exactly 1 time 1415213 (6.77%) aligned >1 times 73.55% overall alignment rate Time searching: 00:13:59 Overall time: 00:13:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 286962 / 11173663 = 0.0257 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:48:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:48:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:48:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:49:02: 1000000 INFO @ Sat, 15 Jan 2022 20:49:07: 2000000 INFO @ Sat, 15 Jan 2022 20:49:13: 3000000 INFO @ Sat, 15 Jan 2022 20:49:18: 4000000 INFO @ Sat, 15 Jan 2022 20:49:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:49:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:49:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:49:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:49:29: 6000000 INFO @ Sat, 15 Jan 2022 20:49:33: 1000000 INFO @ Sat, 15 Jan 2022 20:49:36: 7000000 INFO @ Sat, 15 Jan 2022 20:49:40: 2000000 INFO @ Sat, 15 Jan 2022 20:49:43: 8000000 INFO @ Sat, 15 Jan 2022 20:49:48: 3000000 INFO @ Sat, 15 Jan 2022 20:49:50: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:49:55: 4000000 INFO @ Sat, 15 Jan 2022 20:49:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:49:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:49:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:49:56: 10000000 INFO @ Sat, 15 Jan 2022 20:50:03: 5000000 INFO @ Sat, 15 Jan 2022 20:50:03: 1000000 INFO @ Sat, 15 Jan 2022 20:50:03: 11000000 INFO @ Sat, 15 Jan 2022 20:50:10: 2000000 INFO @ Sat, 15 Jan 2022 20:50:10: 12000000 INFO @ Sat, 15 Jan 2022 20:50:10: 6000000 INFO @ Sat, 15 Jan 2022 20:50:16: 3000000 INFO @ Sat, 15 Jan 2022 20:50:17: 13000000 INFO @ Sat, 15 Jan 2022 20:50:18: 7000000 INFO @ Sat, 15 Jan 2022 20:50:23: 4000000 INFO @ Sat, 15 Jan 2022 20:50:23: 14000000 INFO @ Sat, 15 Jan 2022 20:50:25: 8000000 INFO @ Sat, 15 Jan 2022 20:50:30: 5000000 INFO @ Sat, 15 Jan 2022 20:50:30: 15000000 INFO @ Sat, 15 Jan 2022 20:50:33: 9000000 INFO @ Sat, 15 Jan 2022 20:50:37: 6000000 INFO @ Sat, 15 Jan 2022 20:50:37: 16000000 INFO @ Sat, 15 Jan 2022 20:50:40: 10000000 INFO @ Sat, 15 Jan 2022 20:50:43: 7000000 INFO @ Sat, 15 Jan 2022 20:50:44: 17000000 INFO @ Sat, 15 Jan 2022 20:50:48: 11000000 INFO @ Sat, 15 Jan 2022 20:50:50: 8000000 INFO @ Sat, 15 Jan 2022 20:50:50: 18000000 INFO @ Sat, 15 Jan 2022 20:50:55: 12000000 INFO @ Sat, 15 Jan 2022 20:50:57: 9000000 INFO @ Sat, 15 Jan 2022 20:50:57: 19000000 INFO @ Sat, 15 Jan 2022 20:51:03: 13000000 INFO @ Sat, 15 Jan 2022 20:51:03: 10000000 INFO @ Sat, 15 Jan 2022 20:51:04: 20000000 INFO @ Sat, 15 Jan 2022 20:51:10: 11000000 INFO @ Sat, 15 Jan 2022 20:51:10: 14000000 INFO @ Sat, 15 Jan 2022 20:51:10: 21000000 INFO @ Sat, 15 Jan 2022 20:51:17: 12000000 INFO @ Sat, 15 Jan 2022 20:51:17: 22000000 INFO @ Sat, 15 Jan 2022 20:51:18: 15000000 INFO @ Sat, 15 Jan 2022 20:51:23: 13000000 INFO @ Sat, 15 Jan 2022 20:51:24: 23000000 INFO @ Sat, 15 Jan 2022 20:51:25: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:51:30: 14000000 INFO @ Sat, 15 Jan 2022 20:51:31: 24000000 INFO @ Sat, 15 Jan 2022 20:51:33: 17000000 INFO @ Sat, 15 Jan 2022 20:51:37: 15000000 INFO @ Sat, 15 Jan 2022 20:51:37: 25000000 INFO @ Sat, 15 Jan 2022 20:51:40: 18000000 INFO @ Sat, 15 Jan 2022 20:51:43: 16000000 INFO @ Sat, 15 Jan 2022 20:51:44: 26000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:51:48: 19000000 INFO @ Sat, 15 Jan 2022 20:51:50: 17000000 INFO @ Sat, 15 Jan 2022 20:51:51: 27000000 INFO @ Sat, 15 Jan 2022 20:51:55: 20000000 INFO @ Sat, 15 Jan 2022 20:51:57: 18000000 INFO @ Sat, 15 Jan 2022 20:51:58: 28000000 INFO @ Sat, 15 Jan 2022 20:52:03: 21000000 INFO @ Sat, 15 Jan 2022 20:52:04: 19000000 INFO @ Sat, 15 Jan 2022 20:52:04: 29000000 INFO @ Sat, 15 Jan 2022 20:52:10: 20000000 INFO @ Sat, 15 Jan 2022 20:52:10: 22000000 INFO @ Sat, 15 Jan 2022 20:52:11: 30000000 INFO @ Sat, 15 Jan 2022 20:52:17: 21000000 INFO @ Sat, 15 Jan 2022 20:52:18: 31000000 INFO @ Sat, 15 Jan 2022 20:52:18: 23000000 INFO @ Sat, 15 Jan 2022 20:52:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:52:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:52:19: #1 total tags in treatment: 10482020 INFO @ Sat, 15 Jan 2022 20:52:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:52:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:52:19: #1 tags after filtering in treatment: 7559805 INFO @ Sat, 15 Jan 2022 20:52:19: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 20:52:19: #1 finished! INFO @ Sat, 15 Jan 2022 20:52:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:52:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:52:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:52:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:52:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:52:23: 22000000 INFO @ Sat, 15 Jan 2022 20:52:25: 24000000 INFO @ Sat, 15 Jan 2022 20:52:30: 23000000 INFO @ Sat, 15 Jan 2022 20:52:32: 25000000 INFO @ Sat, 15 Jan 2022 20:52:36: 24000000 INFO @ Sat, 15 Jan 2022 20:52:40: 26000000 INFO @ Sat, 15 Jan 2022 20:52:43: 25000000 INFO @ Sat, 15 Jan 2022 20:52:47: 27000000 INFO @ Sat, 15 Jan 2022 20:52:49: 26000000 INFO @ Sat, 15 Jan 2022 20:52:55: 28000000 INFO @ Sat, 15 Jan 2022 20:52:56: 27000000 INFO @ Sat, 15 Jan 2022 20:53:02: 28000000 INFO @ Sat, 15 Jan 2022 20:53:03: 29000000 INFO @ Sat, 15 Jan 2022 20:53:09: 29000000 INFO @ Sat, 15 Jan 2022 20:53:10: 30000000 INFO @ Sat, 15 Jan 2022 20:53:16: 30000000 INFO @ Sat, 15 Jan 2022 20:53:18: 31000000 INFO @ Sat, 15 Jan 2022 20:53:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:53:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:53:19: #1 total tags in treatment: 10482020 INFO @ Sat, 15 Jan 2022 20:53:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:53:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:53:20: #1 tags after filtering in treatment: 7559805 INFO @ Sat, 15 Jan 2022 20:53:20: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 20:53:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:53:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:53:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:53:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:53:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:53:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:53:22: 31000000 INFO @ Sat, 15 Jan 2022 20:53:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:53:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:53:23: #1 total tags in treatment: 10482020 INFO @ Sat, 15 Jan 2022 20:53:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:53:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:53:23: #1 tags after filtering in treatment: 7559805 INFO @ Sat, 15 Jan 2022 20:53:23: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 20:53:23: #1 finished! INFO @ Sat, 15 Jan 2022 20:53:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:53:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:53:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:53:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:53:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245340/SRX8245340.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling