Job ID = 14520962 SRX = SRX8245338 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15072889 spots for SRR11684549/SRR11684549.sra Written 15072889 spots for SRR11684549/SRR11684549.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:10 15072889 reads; of these: 15072889 (100.00%) were paired; of these: 7433860 (49.32%) aligned concordantly 0 times 6729472 (44.65%) aligned concordantly exactly 1 time 909557 (6.03%) aligned concordantly >1 times ---- 7433860 pairs aligned concordantly 0 times; of these: 442953 (5.96%) aligned discordantly 1 time ---- 6990907 pairs aligned 0 times concordantly or discordantly; of these: 13981814 mates make up the pairs; of these: 7639199 (54.64%) aligned 0 times 5422544 (38.78%) aligned exactly 1 time 920071 (6.58%) aligned >1 times 74.66% overall alignment rate Time searching: 00:12:10 Overall time: 00:12:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 144256 / 8080889 = 0.0179 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:35:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:35:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:35:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:35:06: 1000000 INFO @ Sat, 15 Jan 2022 20:35:12: 2000000 INFO @ Sat, 15 Jan 2022 20:35:19: 3000000 INFO @ Sat, 15 Jan 2022 20:35:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:35:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:35:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:35:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:35:32: 5000000 INFO @ Sat, 15 Jan 2022 20:35:37: 1000000 INFO @ Sat, 15 Jan 2022 20:35:39: 6000000 INFO @ Sat, 15 Jan 2022 20:35:44: 2000000 INFO @ Sat, 15 Jan 2022 20:35:46: 7000000 INFO @ Sat, 15 Jan 2022 20:35:51: 3000000 INFO @ Sat, 15 Jan 2022 20:35:53: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:35:59: 4000000 INFO @ Sat, 15 Jan 2022 20:36:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:36:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:36:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:36:00: 9000000 INFO @ Sat, 15 Jan 2022 20:36:07: 1000000 INFO @ Sat, 15 Jan 2022 20:36:07: 5000000 INFO @ Sat, 15 Jan 2022 20:36:08: 10000000 INFO @ Sat, 15 Jan 2022 20:36:15: 2000000 INFO @ Sat, 15 Jan 2022 20:36:15: 6000000 INFO @ Sat, 15 Jan 2022 20:36:16: 11000000 INFO @ Sat, 15 Jan 2022 20:36:23: 3000000 INFO @ Sat, 15 Jan 2022 20:36:23: 12000000 INFO @ Sat, 15 Jan 2022 20:36:23: 7000000 INFO @ Sat, 15 Jan 2022 20:36:31: 13000000 INFO @ Sat, 15 Jan 2022 20:36:31: 4000000 INFO @ Sat, 15 Jan 2022 20:36:31: 8000000 INFO @ Sat, 15 Jan 2022 20:36:38: 14000000 INFO @ Sat, 15 Jan 2022 20:36:39: 5000000 INFO @ Sat, 15 Jan 2022 20:36:39: 9000000 INFO @ Sat, 15 Jan 2022 20:36:45: 15000000 INFO @ Sat, 15 Jan 2022 20:36:47: 6000000 INFO @ Sat, 15 Jan 2022 20:36:47: 10000000 INFO @ Sat, 15 Jan 2022 20:36:53: 16000000 INFO @ Sat, 15 Jan 2022 20:36:55: 7000000 INFO @ Sat, 15 Jan 2022 20:36:55: 11000000 INFO @ Sat, 15 Jan 2022 20:37:00: 17000000 INFO @ Sat, 15 Jan 2022 20:37:02: 8000000 INFO @ Sat, 15 Jan 2022 20:37:03: 12000000 INFO @ Sat, 15 Jan 2022 20:37:07: 18000000 INFO @ Sat, 15 Jan 2022 20:37:10: 9000000 INFO @ Sat, 15 Jan 2022 20:37:11: 13000000 INFO @ Sat, 15 Jan 2022 20:37:14: 19000000 INFO @ Sat, 15 Jan 2022 20:37:18: 10000000 INFO @ Sat, 15 Jan 2022 20:37:19: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:37:22: 20000000 INFO @ Sat, 15 Jan 2022 20:37:26: 11000000 INFO @ Sat, 15 Jan 2022 20:37:26: 15000000 INFO @ Sat, 15 Jan 2022 20:37:30: 21000000 INFO @ Sat, 15 Jan 2022 20:37:33: 12000000 INFO @ Sat, 15 Jan 2022 20:37:34: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:37:38: 22000000 INFO @ Sat, 15 Jan 2022 20:37:40: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:37:40: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:37:40: #1 total tags in treatment: 7497880 INFO @ Sat, 15 Jan 2022 20:37:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:37:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:37:40: #1 tags after filtering in treatment: 5854602 INFO @ Sat, 15 Jan 2022 20:37:40: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 20:37:40: #1 finished! INFO @ Sat, 15 Jan 2022 20:37:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:37:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:37:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:37:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:37:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:37:41: 13000000 INFO @ Sat, 15 Jan 2022 20:37:41: 17000000 INFO @ Sat, 15 Jan 2022 20:37:48: 14000000 INFO @ Sat, 15 Jan 2022 20:37:48: 18000000 INFO @ Sat, 15 Jan 2022 20:37:55: 15000000 INFO @ Sat, 15 Jan 2022 20:37:56: 19000000 INFO @ Sat, 15 Jan 2022 20:38:02: 16000000 INFO @ Sat, 15 Jan 2022 20:38:03: 20000000 INFO @ Sat, 15 Jan 2022 20:38:08: 17000000 INFO @ Sat, 15 Jan 2022 20:38:11: 21000000 INFO @ Sat, 15 Jan 2022 20:38:15: 18000000 INFO @ Sat, 15 Jan 2022 20:38:19: 22000000 INFO @ Sat, 15 Jan 2022 20:38:21: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:38:21: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:38:21: #1 total tags in treatment: 7497880 INFO @ Sat, 15 Jan 2022 20:38:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:38:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:38:21: #1 tags after filtering in treatment: 5854602 INFO @ Sat, 15 Jan 2022 20:38:21: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 20:38:21: #1 finished! INFO @ Sat, 15 Jan 2022 20:38:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:38:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:38:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:38:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:38:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:38:22: 19000000 INFO @ Sat, 15 Jan 2022 20:38:29: 20000000 INFO @ Sat, 15 Jan 2022 20:38:37: 21000000 INFO @ Sat, 15 Jan 2022 20:38:44: 22000000 INFO @ Sat, 15 Jan 2022 20:38:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:38:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:38:46: #1 total tags in treatment: 7497880 INFO @ Sat, 15 Jan 2022 20:38:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:38:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:38:46: #1 tags after filtering in treatment: 5854602 INFO @ Sat, 15 Jan 2022 20:38:46: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 20:38:46: #1 finished! INFO @ Sat, 15 Jan 2022 20:38:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:38:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:38:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:38:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:38:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245338/SRX8245338.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling