Job ID = 14520961 SRX = SRX8245337 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10416521 spots for SRR11684548/SRR11684548.sra Written 10416521 spots for SRR11684548/SRR11684548.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:17 10416521 reads; of these: 10416521 (100.00%) were paired; of these: 4035567 (38.74%) aligned concordantly 0 times 5640536 (54.15%) aligned concordantly exactly 1 time 740418 (7.11%) aligned concordantly >1 times ---- 4035567 pairs aligned concordantly 0 times; of these: 336545 (8.34%) aligned discordantly 1 time ---- 3699022 pairs aligned 0 times concordantly or discordantly; of these: 7398044 mates make up the pairs; of these: 4316854 (58.35%) aligned 0 times 2614993 (35.35%) aligned exactly 1 time 466197 (6.30%) aligned >1 times 79.28% overall alignment rate Time searching: 00:08:17 Overall time: 00:08:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 140880 / 6716709 = 0.0210 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:21: 1000000 INFO @ Sat, 15 Jan 2022 20:31:27: 2000000 INFO @ Sat, 15 Jan 2022 20:31:32: 3000000 INFO @ Sat, 15 Jan 2022 20:31:37: 4000000 INFO @ Sat, 15 Jan 2022 20:31:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:48: 6000000 INFO @ Sat, 15 Jan 2022 20:31:52: 1000000 INFO @ Sat, 15 Jan 2022 20:31:53: 7000000 INFO @ Sat, 15 Jan 2022 20:31:57: 2000000 INFO @ Sat, 15 Jan 2022 20:31:59: 8000000 INFO @ Sat, 15 Jan 2022 20:32:03: 3000000 INFO @ Sat, 15 Jan 2022 20:32:05: 9000000 INFO @ Sat, 15 Jan 2022 20:32:08: 4000000 INFO @ Sat, 15 Jan 2022 20:32:10: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:32:14: 5000000 INFO @ Sat, 15 Jan 2022 20:32:16: 11000000 INFO @ Sat, 15 Jan 2022 20:32:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:32:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:32:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:32:20: 6000000 INFO @ Sat, 15 Jan 2022 20:32:22: 12000000 INFO @ Sat, 15 Jan 2022 20:32:22: 1000000 INFO @ Sat, 15 Jan 2022 20:32:26: 7000000 INFO @ Sat, 15 Jan 2022 20:32:28: 13000000 INFO @ Sat, 15 Jan 2022 20:32:29: 2000000 INFO @ Sat, 15 Jan 2022 20:32:32: 8000000 INFO @ Sat, 15 Jan 2022 20:32:34: 14000000 INFO @ Sat, 15 Jan 2022 20:32:35: 3000000 INFO @ Sat, 15 Jan 2022 20:32:38: 9000000 INFO @ Sat, 15 Jan 2022 20:32:40: 15000000 INFO @ Sat, 15 Jan 2022 20:32:41: 4000000 INFO @ Sat, 15 Jan 2022 20:32:44: 10000000 INFO @ Sat, 15 Jan 2022 20:32:46: 16000000 INFO @ Sat, 15 Jan 2022 20:32:47: 5000000 INFO @ Sat, 15 Jan 2022 20:32:47: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:47: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:47: #1 total tags in treatment: 6243419 INFO @ Sat, 15 Jan 2022 20:32:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:47: #1 tags after filtering in treatment: 4997600 INFO @ Sat, 15 Jan 2022 20:32:47: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:32:47: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:32:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:32:50: 11000000 INFO @ Sat, 15 Jan 2022 20:32:52: 6000000 INFO @ Sat, 15 Jan 2022 20:32:56: 12000000 INFO @ Sat, 15 Jan 2022 20:32:58: 7000000 INFO @ Sat, 15 Jan 2022 20:33:01: 13000000 INFO @ Sat, 15 Jan 2022 20:33:03: 8000000 INFO @ Sat, 15 Jan 2022 20:33:07: 14000000 INFO @ Sat, 15 Jan 2022 20:33:09: 9000000 INFO @ Sat, 15 Jan 2022 20:33:13: 15000000 INFO @ Sat, 15 Jan 2022 20:33:14: 10000000 INFO @ Sat, 15 Jan 2022 20:33:18: 16000000 INFO @ Sat, 15 Jan 2022 20:33:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:33:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:33:19: #1 total tags in treatment: 6243419 INFO @ Sat, 15 Jan 2022 20:33:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:33:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:33:19: 11000000 INFO @ Sat, 15 Jan 2022 20:33:20: #1 tags after filtering in treatment: 4997600 INFO @ Sat, 15 Jan 2022 20:33:20: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:33:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:33:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:33:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:33:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:33:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:33:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:33:25: 12000000 INFO @ Sat, 15 Jan 2022 20:33:30: 13000000 INFO @ Sat, 15 Jan 2022 20:33:35: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:33:39: 15000000 INFO @ Sat, 15 Jan 2022 20:33:45: 16000000 INFO @ Sat, 15 Jan 2022 20:33:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:33:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:33:46: #1 total tags in treatment: 6243419 INFO @ Sat, 15 Jan 2022 20:33:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:33:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:33:46: #1 tags after filtering in treatment: 4997600 INFO @ Sat, 15 Jan 2022 20:33:46: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:33:46: #1 finished! INFO @ Sat, 15 Jan 2022 20:33:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:33:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:33:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:33:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:33:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245337/SRX8245337.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling