Job ID = 14520960 SRX = SRX8245336 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11300577 spots for SRR11684547/SRR11684547.sra Written 11300577 spots for SRR11684547/SRR11684547.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:02 11300577 reads; of these: 11300577 (100.00%) were paired; of these: 4816152 (42.62%) aligned concordantly 0 times 5699937 (50.44%) aligned concordantly exactly 1 time 784488 (6.94%) aligned concordantly >1 times ---- 4816152 pairs aligned concordantly 0 times; of these: 375757 (7.80%) aligned discordantly 1 time ---- 4440395 pairs aligned 0 times concordantly or discordantly; of these: 8880790 mates make up the pairs; of these: 5044250 (56.80%) aligned 0 times 3244731 (36.54%) aligned exactly 1 time 591809 (6.66%) aligned >1 times 77.68% overall alignment rate Time searching: 00:06:02 Overall time: 00:06:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 155613 / 6859361 = 0.0227 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:23:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:23:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:23:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:23:43: 1000000 INFO @ Sat, 15 Jan 2022 20:23:48: 2000000 INFO @ Sat, 15 Jan 2022 20:23:53: 3000000 INFO @ Sat, 15 Jan 2022 20:23:58: 4000000 INFO @ Sat, 15 Jan 2022 20:24:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:24:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:24:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:24:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:08: 6000000 INFO @ Sat, 15 Jan 2022 20:24:15: 7000000 INFO @ Sat, 15 Jan 2022 20:24:15: 1000000 INFO @ Sat, 15 Jan 2022 20:24:21: 8000000 INFO @ Sat, 15 Jan 2022 20:24:22: 2000000 INFO @ Sat, 15 Jan 2022 20:24:27: 9000000 INFO @ Sat, 15 Jan 2022 20:24:29: 3000000 INFO @ Sat, 15 Jan 2022 20:24:33: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:24:37: 4000000 INFO @ Sat, 15 Jan 2022 20:24:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:24:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:24:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:39: 11000000 INFO @ Sat, 15 Jan 2022 20:24:44: 5000000 INFO @ Sat, 15 Jan 2022 20:24:45: 1000000 INFO @ Sat, 15 Jan 2022 20:24:46: 12000000 INFO @ Sat, 15 Jan 2022 20:24:52: 6000000 INFO @ Sat, 15 Jan 2022 20:24:54: 2000000 INFO @ Sat, 15 Jan 2022 20:24:55: 13000000 INFO @ Sat, 15 Jan 2022 20:24:59: 7000000 INFO @ Sat, 15 Jan 2022 20:25:01: 14000000 INFO @ Sat, 15 Jan 2022 20:25:01: 3000000 INFO @ Sat, 15 Jan 2022 20:25:11: 15000000 INFO @ Sat, 15 Jan 2022 20:25:11: 8000000 INFO @ Sat, 15 Jan 2022 20:25:11: 4000000 INFO @ Sat, 15 Jan 2022 20:25:17: 16000000 INFO @ Sat, 15 Jan 2022 20:25:18: 9000000 INFO @ Sat, 15 Jan 2022 20:25:19: 5000000 INFO @ Sat, 15 Jan 2022 20:25:24: 17000000 INFO @ Sat, 15 Jan 2022 20:25:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:25:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:25:25: #1 total tags in treatment: 6332545 INFO @ Sat, 15 Jan 2022 20:25:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:25:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:25:25: #1 tags after filtering in treatment: 5070127 INFO @ Sat, 15 Jan 2022 20:25:25: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:25:25: #1 finished! INFO @ Sat, 15 Jan 2022 20:25:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:25:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:25:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:25:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:25:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:25:26: 10000000 INFO @ Sat, 15 Jan 2022 20:25:28: 6000000 INFO @ Sat, 15 Jan 2022 20:25:33: 11000000 INFO @ Sat, 15 Jan 2022 20:25:35: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:25:42: 12000000 INFO @ Sat, 15 Jan 2022 20:25:42: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:25:48: 13000000 INFO @ Sat, 15 Jan 2022 20:25:49: 9000000 INFO @ Sat, 15 Jan 2022 20:25:55: 14000000 INFO @ Sat, 15 Jan 2022 20:25:56: 10000000 INFO @ Sat, 15 Jan 2022 20:26:01: 15000000 INFO @ Sat, 15 Jan 2022 20:26:03: 11000000 INFO @ Sat, 15 Jan 2022 20:26:08: 16000000 INFO @ Sat, 15 Jan 2022 20:26:11: 12000000 INFO @ Sat, 15 Jan 2022 20:26:14: 17000000 INFO @ Sat, 15 Jan 2022 20:26:15: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:26:15: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:26:15: #1 total tags in treatment: 6332545 INFO @ Sat, 15 Jan 2022 20:26:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:16: #1 tags after filtering in treatment: 5070127 INFO @ Sat, 15 Jan 2022 20:26:16: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:26:16: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:26:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:26:18: 13000000 INFO @ Sat, 15 Jan 2022 20:26:24: 14000000 INFO @ Sat, 15 Jan 2022 20:26:31: 15000000 INFO @ Sat, 15 Jan 2022 20:26:37: 16000000 INFO @ Sat, 15 Jan 2022 20:26:45: 17000000 INFO @ Sat, 15 Jan 2022 20:26:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:26:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:26:46: #1 total tags in treatment: 6332545 INFO @ Sat, 15 Jan 2022 20:26:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:46: #1 tags after filtering in treatment: 5070127 INFO @ Sat, 15 Jan 2022 20:26:46: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:26:46: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:26:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245336/SRX8245336.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling