Job ID = 14520959 SRX = SRX8245335 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17993885 spots for SRR11684546/SRR11684546.sra Written 17993885 spots for SRR11684546/SRR11684546.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:29 17993885 reads; of these: 17993885 (100.00%) were paired; of these: 6566870 (36.50%) aligned concordantly 0 times 9777289 (54.34%) aligned concordantly exactly 1 time 1649726 (9.17%) aligned concordantly >1 times ---- 6566870 pairs aligned concordantly 0 times; of these: 39311 (0.60%) aligned discordantly 1 time ---- 6527559 pairs aligned 0 times concordantly or discordantly; of these: 13055118 mates make up the pairs; of these: 7554215 (57.86%) aligned 0 times 4669212 (35.77%) aligned exactly 1 time 831691 (6.37%) aligned >1 times 79.01% overall alignment rate Time searching: 00:10:29 Overall time: 00:10:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 363879 / 11464594 = 0.0317 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:27: 1000000 INFO @ Sat, 15 Jan 2022 20:33:32: 2000000 INFO @ Sat, 15 Jan 2022 20:33:38: 3000000 INFO @ Sat, 15 Jan 2022 20:33:43: 4000000 INFO @ Sat, 15 Jan 2022 20:33:48: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:54: 6000000 INFO @ Sat, 15 Jan 2022 20:33:58: 1000000 INFO @ Sat, 15 Jan 2022 20:34:01: 7000000 INFO @ Sat, 15 Jan 2022 20:34:06: 2000000 INFO @ Sat, 15 Jan 2022 20:34:08: 8000000 INFO @ Sat, 15 Jan 2022 20:34:14: 3000000 INFO @ Sat, 15 Jan 2022 20:34:14: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:34:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:34:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:34:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:34:21: 10000000 INFO @ Sat, 15 Jan 2022 20:34:22: 4000000 INFO @ Sat, 15 Jan 2022 20:34:28: 11000000 INFO @ Sat, 15 Jan 2022 20:34:28: 1000000 INFO @ Sat, 15 Jan 2022 20:34:30: 5000000 INFO @ Sat, 15 Jan 2022 20:34:35: 12000000 INFO @ Sat, 15 Jan 2022 20:34:35: 2000000 INFO @ Sat, 15 Jan 2022 20:34:38: 6000000 INFO @ Sat, 15 Jan 2022 20:34:42: 13000000 INFO @ Sat, 15 Jan 2022 20:34:42: 3000000 INFO @ Sat, 15 Jan 2022 20:34:46: 7000000 INFO @ Sat, 15 Jan 2022 20:34:49: 14000000 INFO @ Sat, 15 Jan 2022 20:34:49: 4000000 INFO @ Sat, 15 Jan 2022 20:34:53: 8000000 INFO @ Sat, 15 Jan 2022 20:34:55: 15000000 INFO @ Sat, 15 Jan 2022 20:34:55: 5000000 INFO @ Sat, 15 Jan 2022 20:35:01: 9000000 INFO @ Sat, 15 Jan 2022 20:35:02: 16000000 INFO @ Sat, 15 Jan 2022 20:35:02: 6000000 INFO @ Sat, 15 Jan 2022 20:35:09: 10000000 INFO @ Sat, 15 Jan 2022 20:35:09: 7000000 INFO @ Sat, 15 Jan 2022 20:35:09: 17000000 INFO @ Sat, 15 Jan 2022 20:35:16: 8000000 INFO @ Sat, 15 Jan 2022 20:35:16: 18000000 INFO @ Sat, 15 Jan 2022 20:35:17: 11000000 INFO @ Sat, 15 Jan 2022 20:35:23: 9000000 INFO @ Sat, 15 Jan 2022 20:35:23: 19000000 INFO @ Sat, 15 Jan 2022 20:35:25: 12000000 INFO @ Sat, 15 Jan 2022 20:35:30: 10000000 INFO @ Sat, 15 Jan 2022 20:35:30: 20000000 INFO @ Sat, 15 Jan 2022 20:35:33: 13000000 INFO @ Sat, 15 Jan 2022 20:35:37: 11000000 INFO @ Sat, 15 Jan 2022 20:35:37: 21000000 INFO @ Sat, 15 Jan 2022 20:35:41: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:35:43: 12000000 INFO @ Sat, 15 Jan 2022 20:35:43: 22000000 INFO @ Sat, 15 Jan 2022 20:35:48: 15000000 INFO @ Sat, 15 Jan 2022 20:35:51: 23000000 INFO @ Sat, 15 Jan 2022 20:35:51: 13000000 INFO @ Sat, 15 Jan 2022 20:35:56: 16000000 INFO @ Sat, 15 Jan 2022 20:35:58: 14000000 INFO @ Sat, 15 Jan 2022 20:35:58: 24000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:36:04: 17000000 INFO @ Sat, 15 Jan 2022 20:36:05: 25000000 INFO @ Sat, 15 Jan 2022 20:36:05: 15000000 INFO @ Sat, 15 Jan 2022 20:36:12: 26000000 INFO @ Sat, 15 Jan 2022 20:36:12: 16000000 INFO @ Sat, 15 Jan 2022 20:36:12: 18000000 INFO @ Sat, 15 Jan 2022 20:36:19: 17000000 INFO @ Sat, 15 Jan 2022 20:36:19: 27000000 INFO @ Sat, 15 Jan 2022 20:36:19: 19000000 INFO @ Sat, 15 Jan 2022 20:36:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:36:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:36:24: #1 total tags in treatment: 11063208 INFO @ Sat, 15 Jan 2022 20:36:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:36:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:36:24: #1 tags after filtering in treatment: 7730012 INFO @ Sat, 15 Jan 2022 20:36:24: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:36:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:36:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:36:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:36:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:36:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:36:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:36:25: 18000000 INFO @ Sat, 15 Jan 2022 20:36:27: 20000000 INFO @ Sat, 15 Jan 2022 20:36:32: 19000000 INFO @ Sat, 15 Jan 2022 20:36:35: 21000000 INFO @ Sat, 15 Jan 2022 20:36:39: 20000000 INFO @ Sat, 15 Jan 2022 20:36:43: 22000000 INFO @ Sat, 15 Jan 2022 20:36:46: 21000000 INFO @ Sat, 15 Jan 2022 20:36:51: 23000000 INFO @ Sat, 15 Jan 2022 20:36:53: 22000000 INFO @ Sat, 15 Jan 2022 20:36:58: 24000000 INFO @ Sat, 15 Jan 2022 20:37:00: 23000000 INFO @ Sat, 15 Jan 2022 20:37:06: 25000000 INFO @ Sat, 15 Jan 2022 20:37:07: 24000000 INFO @ Sat, 15 Jan 2022 20:37:14: 25000000 INFO @ Sat, 15 Jan 2022 20:37:14: 26000000 INFO @ Sat, 15 Jan 2022 20:37:20: 26000000 INFO @ Sat, 15 Jan 2022 20:37:21: 27000000 INFO @ Sat, 15 Jan 2022 20:37:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:37:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:37:27: #1 total tags in treatment: 11063208 INFO @ Sat, 15 Jan 2022 20:37:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:37:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:37:27: #1 tags after filtering in treatment: 7730012 INFO @ Sat, 15 Jan 2022 20:37:27: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:37:27: #1 finished! INFO @ Sat, 15 Jan 2022 20:37:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:37:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:37:27: 27000000 INFO @ Sat, 15 Jan 2022 20:37:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:37:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:37:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:37:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:37:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:37:31: #1 total tags in treatment: 11063208 INFO @ Sat, 15 Jan 2022 20:37:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:37:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:37:32: #1 tags after filtering in treatment: 7730012 INFO @ Sat, 15 Jan 2022 20:37:32: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:37:32: #1 finished! INFO @ Sat, 15 Jan 2022 20:37:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:37:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:37:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:37:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:37:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245335/SRX8245335.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling