Job ID = 14520958 SRX = SRX8245334 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20151963 spots for SRR11684545/SRR11684545.sra Written 20151963 spots for SRR11684545/SRR11684545.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:37 20151963 reads; of these: 20151963 (100.00%) were paired; of these: 12519323 (62.12%) aligned concordantly 0 times 6630871 (32.90%) aligned concordantly exactly 1 time 1001769 (4.97%) aligned concordantly >1 times ---- 12519323 pairs aligned concordantly 0 times; of these: 36887 (0.29%) aligned discordantly 1 time ---- 12482436 pairs aligned 0 times concordantly or discordantly; of these: 24964872 mates make up the pairs; of these: 13624475 (54.57%) aligned 0 times 9791062 (39.22%) aligned exactly 1 time 1549335 (6.21%) aligned >1 times 66.20% overall alignment rate Time searching: 00:10:37 Overall time: 00:10:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 171745 / 7668458 = 0.0224 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:27: 1000000 INFO @ Sat, 15 Jan 2022 20:33:32: 2000000 INFO @ Sat, 15 Jan 2022 20:33:37: 3000000 INFO @ Sat, 15 Jan 2022 20:33:42: 4000000 INFO @ Sat, 15 Jan 2022 20:33:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:53: 6000000 INFO @ Sat, 15 Jan 2022 20:33:59: 1000000 INFO @ Sat, 15 Jan 2022 20:33:59: 7000000 INFO @ Sat, 15 Jan 2022 20:34:06: 2000000 INFO @ Sat, 15 Jan 2022 20:34:06: 8000000 INFO @ Sat, 15 Jan 2022 20:34:13: 9000000 INFO @ Sat, 15 Jan 2022 20:34:13: 3000000 BedGraph に変換中... INFO @ Sat, 15 Jan 2022 20:34:20: 10000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:34:20: 4000000 INFO @ Sat, 15 Jan 2022 20:34:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:34:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:34:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:34:27: 11000000 INFO @ Sat, 15 Jan 2022 20:34:28: 5000000 INFO @ Sat, 15 Jan 2022 20:34:28: 1000000 INFO @ Sat, 15 Jan 2022 20:34:33: 12000000 INFO @ Sat, 15 Jan 2022 20:34:35: 6000000 INFO @ Sat, 15 Jan 2022 20:34:35: 2000000 INFO @ Sat, 15 Jan 2022 20:34:40: 13000000 INFO @ Sat, 15 Jan 2022 20:34:42: 7000000 INFO @ Sat, 15 Jan 2022 20:34:42: 3000000 INFO @ Sat, 15 Jan 2022 20:34:47: 14000000 INFO @ Sat, 15 Jan 2022 20:34:49: 4000000 INFO @ Sat, 15 Jan 2022 20:34:49: 8000000 INFO @ Sat, 15 Jan 2022 20:34:54: 15000000 INFO @ Sat, 15 Jan 2022 20:34:56: 5000000 INFO @ Sat, 15 Jan 2022 20:34:56: 9000000 INFO @ Sat, 15 Jan 2022 20:35:01: 16000000 INFO @ Sat, 15 Jan 2022 20:35:02: 6000000 INFO @ Sat, 15 Jan 2022 20:35:03: 10000000 INFO @ Sat, 15 Jan 2022 20:35:08: 17000000 INFO @ Sat, 15 Jan 2022 20:35:09: 7000000 INFO @ Sat, 15 Jan 2022 20:35:11: 11000000 INFO @ Sat, 15 Jan 2022 20:35:15: 18000000 INFO @ Sat, 15 Jan 2022 20:35:16: 8000000 INFO @ Sat, 15 Jan 2022 20:35:18: 12000000 INFO @ Sat, 15 Jan 2022 20:35:22: 19000000 INFO @ Sat, 15 Jan 2022 20:35:23: 9000000 INFO @ Sat, 15 Jan 2022 20:35:26: 13000000 INFO @ Sat, 15 Jan 2022 20:35:29: 20000000 INFO @ Sat, 15 Jan 2022 20:35:31: 10000000 INFO @ Sat, 15 Jan 2022 20:35:33: 14000000 INFO @ Sat, 15 Jan 2022 20:35:36: 21000000 INFO @ Sat, 15 Jan 2022 20:35:38: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:35:40: 15000000 INFO @ Sat, 15 Jan 2022 20:35:42: 22000000 INFO @ Sat, 15 Jan 2022 20:35:44: 12000000 INFO @ Sat, 15 Jan 2022 20:35:47: 16000000 INFO @ Sat, 15 Jan 2022 20:35:49: 23000000 INFO @ Sat, 15 Jan 2022 20:35:51: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:35:54: 17000000 INFO @ Sat, 15 Jan 2022 20:35:56: 24000000 INFO @ Sat, 15 Jan 2022 20:35:58: 14000000 INFO @ Sat, 15 Jan 2022 20:36:02: 18000000 INFO @ Sat, 15 Jan 2022 20:36:03: 25000000 INFO @ Sat, 15 Jan 2022 20:36:05: 15000000 INFO @ Sat, 15 Jan 2022 20:36:09: 19000000 INFO @ Sat, 15 Jan 2022 20:36:10: 26000000 INFO @ Sat, 15 Jan 2022 20:36:12: 16000000 INFO @ Sat, 15 Jan 2022 20:36:12: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:36:12: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:36:12: #1 total tags in treatment: 7460987 INFO @ Sat, 15 Jan 2022 20:36:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:36:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:36:12: #1 tags after filtering in treatment: 5663477 INFO @ Sat, 15 Jan 2022 20:36:12: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:36:12: #1 finished! INFO @ Sat, 15 Jan 2022 20:36:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:36:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:36:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:36:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:36:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:36:16: 20000000 INFO @ Sat, 15 Jan 2022 20:36:18: 17000000 INFO @ Sat, 15 Jan 2022 20:36:23: 21000000 INFO @ Sat, 15 Jan 2022 20:36:25: 18000000 INFO @ Sat, 15 Jan 2022 20:36:31: 22000000 INFO @ Sat, 15 Jan 2022 20:36:32: 19000000 INFO @ Sat, 15 Jan 2022 20:36:38: 23000000 INFO @ Sat, 15 Jan 2022 20:36:39: 20000000 INFO @ Sat, 15 Jan 2022 20:36:45: 21000000 INFO @ Sat, 15 Jan 2022 20:36:45: 24000000 INFO @ Sat, 15 Jan 2022 20:36:52: 22000000 INFO @ Sat, 15 Jan 2022 20:36:53: 25000000 INFO @ Sat, 15 Jan 2022 20:36:59: 23000000 INFO @ Sat, 15 Jan 2022 20:37:00: 26000000 INFO @ Sat, 15 Jan 2022 20:37:02: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:37:02: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:37:02: #1 total tags in treatment: 7460987 INFO @ Sat, 15 Jan 2022 20:37:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:37:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:37:02: #1 tags after filtering in treatment: 5663477 INFO @ Sat, 15 Jan 2022 20:37:02: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:37:02: #1 finished! INFO @ Sat, 15 Jan 2022 20:37:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:37:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:37:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:37:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:37:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:37:05: 24000000 INFO @ Sat, 15 Jan 2022 20:37:12: 25000000 INFO @ Sat, 15 Jan 2022 20:37:19: 26000000 INFO @ Sat, 15 Jan 2022 20:37:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:37:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:37:20: #1 total tags in treatment: 7460987 INFO @ Sat, 15 Jan 2022 20:37:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:37:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:37:21: #1 tags after filtering in treatment: 5663477 INFO @ Sat, 15 Jan 2022 20:37:21: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:37:21: #1 finished! INFO @ Sat, 15 Jan 2022 20:37:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:37:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:37:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:37:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:37:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245334/SRX8245334.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling