Job ID = 14520921 SRX = SRX8245327 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15261097 spots for SRR11684522/SRR11684522.sra Written 15261097 spots for SRR11684522/SRR11684522.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:23 15261097 reads; of these: 15261097 (100.00%) were paired; of these: 11273920 (73.87%) aligned concordantly 0 times 3495192 (22.90%) aligned concordantly exactly 1 time 491985 (3.22%) aligned concordantly >1 times ---- 11273920 pairs aligned concordantly 0 times; of these: 137105 (1.22%) aligned discordantly 1 time ---- 11136815 pairs aligned 0 times concordantly or discordantly; of these: 22273630 mates make up the pairs; of these: 11470654 (51.50%) aligned 0 times 9345151 (41.96%) aligned exactly 1 time 1457825 (6.55%) aligned >1 times 62.42% overall alignment rate Time searching: 00:07:23 Overall time: 00:07:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 100960 / 4123709 = 0.0245 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:21:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:21:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:21:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:21:39: 1000000 INFO @ Sat, 15 Jan 2022 20:21:43: 2000000 INFO @ Sat, 15 Jan 2022 20:21:48: 3000000 INFO @ Sat, 15 Jan 2022 20:21:52: 4000000 INFO @ Sat, 15 Jan 2022 20:21:56: 5000000 INFO @ Sat, 15 Jan 2022 20:22:01: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:22:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:22:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:22:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:22:05: 7000000 INFO @ Sat, 15 Jan 2022 20:22:10: 1000000 INFO @ Sat, 15 Jan 2022 20:22:10: 8000000 INFO @ Sat, 15 Jan 2022 20:22:15: 9000000 INFO @ Sat, 15 Jan 2022 20:22:15: 2000000 INFO @ Sat, 15 Jan 2022 20:22:20: 10000000 INFO @ Sat, 15 Jan 2022 20:22:21: 3000000 INFO @ Sat, 15 Jan 2022 20:22:25: 11000000 INFO @ Sat, 15 Jan 2022 20:22:27: 4000000 INFO @ Sat, 15 Jan 2022 20:22:30: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:22:32: 5000000 INFO @ Sat, 15 Jan 2022 20:22:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:22:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:22:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:22:35: 13000000 INFO @ Sat, 15 Jan 2022 20:22:38: 6000000 INFO @ Sat, 15 Jan 2022 20:22:39: 1000000 INFO @ Sat, 15 Jan 2022 20:22:40: 14000000 INFO @ Sat, 15 Jan 2022 20:22:44: 2000000 INFO @ Sat, 15 Jan 2022 20:22:44: 7000000 INFO @ Sat, 15 Jan 2022 20:22:45: 15000000 INFO @ Sat, 15 Jan 2022 20:22:49: 3000000 INFO @ Sat, 15 Jan 2022 20:22:50: 16000000 INFO @ Sat, 15 Jan 2022 20:22:50: 8000000 INFO @ Sat, 15 Jan 2022 20:22:54: 4000000 INFO @ Sat, 15 Jan 2022 20:22:55: 17000000 INFO @ Sat, 15 Jan 2022 20:22:56: 9000000 INFO @ Sat, 15 Jan 2022 20:22:59: 5000000 INFO @ Sat, 15 Jan 2022 20:23:00: 18000000 INFO @ Sat, 15 Jan 2022 20:23:01: 10000000 INFO @ Sat, 15 Jan 2022 20:23:04: #1 tag size is determined as 28 bps INFO @ Sat, 15 Jan 2022 20:23:04: #1 tag size = 28 INFO @ Sat, 15 Jan 2022 20:23:04: #1 total tags in treatment: 3887600 INFO @ Sat, 15 Jan 2022 20:23:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:23:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:23:04: #1 tags after filtering in treatment: 3292395 INFO @ Sat, 15 Jan 2022 20:23:04: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 15 Jan 2022 20:23:04: #1 finished! INFO @ Sat, 15 Jan 2022 20:23:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:23:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:23:04: 6000000 INFO @ Sat, 15 Jan 2022 20:23:04: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 20:23:04: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:23:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:23:07: 11000000 INFO @ Sat, 15 Jan 2022 20:23:09: 7000000 INFO @ Sat, 15 Jan 2022 20:23:13: 12000000 INFO @ Sat, 15 Jan 2022 20:23:14: 8000000 INFO @ Sat, 15 Jan 2022 20:23:19: 9000000 INFO @ Sat, 15 Jan 2022 20:23:19: 13000000 INFO @ Sat, 15 Jan 2022 20:23:24: 10000000 INFO @ Sat, 15 Jan 2022 20:23:25: 14000000 INFO @ Sat, 15 Jan 2022 20:23:28: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:23:30: 15000000 INFO @ Sat, 15 Jan 2022 20:23:33: 12000000 INFO @ Sat, 15 Jan 2022 20:23:36: 16000000 INFO @ Sat, 15 Jan 2022 20:23:38: 13000000 INFO @ Sat, 15 Jan 2022 20:23:42: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:23:43: 14000000 INFO @ Sat, 15 Jan 2022 20:23:48: 18000000 INFO @ Sat, 15 Jan 2022 20:23:48: 15000000 INFO @ Sat, 15 Jan 2022 20:23:52: #1 tag size is determined as 28 bps INFO @ Sat, 15 Jan 2022 20:23:52: #1 tag size = 28 INFO @ Sat, 15 Jan 2022 20:23:52: #1 total tags in treatment: 3887600 INFO @ Sat, 15 Jan 2022 20:23:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:23:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:23:52: #1 tags after filtering in treatment: 3292395 INFO @ Sat, 15 Jan 2022 20:23:52: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 15 Jan 2022 20:23:52: #1 finished! INFO @ Sat, 15 Jan 2022 20:23:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:23:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:23:53: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 20:23:53: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:23:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:23:53: 16000000 INFO @ Sat, 15 Jan 2022 20:23:57: 17000000 INFO @ Sat, 15 Jan 2022 20:24:01: 18000000 INFO @ Sat, 15 Jan 2022 20:24:05: #1 tag size is determined as 28 bps INFO @ Sat, 15 Jan 2022 20:24:05: #1 tag size = 28 INFO @ Sat, 15 Jan 2022 20:24:05: #1 total tags in treatment: 3887600 INFO @ Sat, 15 Jan 2022 20:24:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:24:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:24:05: #1 tags after filtering in treatment: 3292395 INFO @ Sat, 15 Jan 2022 20:24:05: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 15 Jan 2022 20:24:05: #1 finished! INFO @ Sat, 15 Jan 2022 20:24:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:24:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:24:05: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 20:24:05: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:24:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245327/SRX8245327.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling