Job ID = 14520920 SRX = SRX8245326 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13262344 spots for SRR11684521/SRR11684521.sra Written 13262344 spots for SRR11684521/SRR11684521.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:29 13262344 reads; of these: 13262344 (100.00%) were paired; of these: 8879731 (66.95%) aligned concordantly 0 times 3833924 (28.91%) aligned concordantly exactly 1 time 548689 (4.14%) aligned concordantly >1 times ---- 8879731 pairs aligned concordantly 0 times; of these: 152438 (1.72%) aligned discordantly 1 time ---- 8727293 pairs aligned 0 times concordantly or discordantly; of these: 17454586 mates make up the pairs; of these: 9024564 (51.70%) aligned 0 times 7264659 (41.62%) aligned exactly 1 time 1165363 (6.68%) aligned >1 times 65.98% overall alignment rate Time searching: 00:09:29 Overall time: 00:09:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 121422 / 4534399 = 0.0268 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:27:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:27:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:27:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:02: 1000000 INFO @ Sat, 15 Jan 2022 20:28:10: 2000000 INFO @ Sat, 15 Jan 2022 20:28:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:27: 4000000 INFO @ Sat, 15 Jan 2022 20:28:31: 1000000 INFO @ Sat, 15 Jan 2022 20:28:36: 5000000 INFO @ Sat, 15 Jan 2022 20:28:40: 2000000 INFO @ Sat, 15 Jan 2022 20:28:44: 6000000 INFO @ Sat, 15 Jan 2022 20:28:49: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:52: 7000000 INFO @ Sat, 15 Jan 2022 20:28:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:58: 4000000 INFO @ Sat, 15 Jan 2022 20:29:01: 1000000 INFO @ Sat, 15 Jan 2022 20:29:01: 8000000 INFO @ Sat, 15 Jan 2022 20:29:07: 5000000 INFO @ Sat, 15 Jan 2022 20:29:08: 2000000 INFO @ Sat, 15 Jan 2022 20:29:08: 9000000 INFO @ Sat, 15 Jan 2022 20:29:15: 3000000 INFO @ Sat, 15 Jan 2022 20:29:16: 10000000 INFO @ Sat, 15 Jan 2022 20:29:16: 6000000 INFO @ Sat, 15 Jan 2022 20:29:22: 4000000 INFO @ Sat, 15 Jan 2022 20:29:23: 11000000 INFO @ Sat, 15 Jan 2022 20:29:25: 7000000 INFO @ Sat, 15 Jan 2022 20:29:29: 5000000 INFO @ Sat, 15 Jan 2022 20:29:31: 12000000 INFO @ Sat, 15 Jan 2022 20:29:34: 8000000 INFO @ Sat, 15 Jan 2022 20:29:35: 6000000 INFO @ Sat, 15 Jan 2022 20:29:40: 13000000 INFO @ Sat, 15 Jan 2022 20:29:42: 7000000 INFO @ Sat, 15 Jan 2022 20:29:44: 9000000 INFO @ Sat, 15 Jan 2022 20:29:49: 8000000 INFO @ Sat, 15 Jan 2022 20:29:49: 14000000 INFO @ Sat, 15 Jan 2022 20:29:53: 10000000 INFO @ Sat, 15 Jan 2022 20:29:55: 9000000 INFO @ Sat, 15 Jan 2022 20:29:57: 15000000 INFO @ Sat, 15 Jan 2022 20:30:02: 10000000 INFO @ Sat, 15 Jan 2022 20:30:02: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:30:06: 16000000 INFO @ Sat, 15 Jan 2022 20:30:08: 11000000 INFO @ Sat, 15 Jan 2022 20:30:11: 12000000 INFO @ Sat, 15 Jan 2022 20:30:14: 17000000 INFO @ Sat, 15 Jan 2022 20:30:15: 12000000 INFO @ Sat, 15 Jan 2022 20:30:16: #1 tag size is determined as 33 bps INFO @ Sat, 15 Jan 2022 20:30:16: #1 tag size = 33 INFO @ Sat, 15 Jan 2022 20:30:16: #1 total tags in treatment: 4262765 INFO @ Sat, 15 Jan 2022 20:30:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:16: #1 tags after filtering in treatment: 3557016 INFO @ Sat, 15 Jan 2022 20:30:16: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:30:16: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:30:17: #2 number of paired peaks: 31 WARNING @ Sat, 15 Jan 2022 20:30:17: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:30:19: 13000000 INFO @ Sat, 15 Jan 2022 20:30:21: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:30:28: 14000000 INFO @ Sat, 15 Jan 2022 20:30:29: 14000000 INFO @ Sat, 15 Jan 2022 20:30:35: 15000000 INFO @ Sat, 15 Jan 2022 20:30:38: 15000000 INFO @ Sat, 15 Jan 2022 20:30:41: 16000000 INFO @ Sat, 15 Jan 2022 20:30:47: 16000000 INFO @ Sat, 15 Jan 2022 20:30:47: 17000000 INFO @ Sat, 15 Jan 2022 20:30:49: #1 tag size is determined as 33 bps INFO @ Sat, 15 Jan 2022 20:30:49: #1 tag size = 33 INFO @ Sat, 15 Jan 2022 20:30:49: #1 total tags in treatment: 4262765 INFO @ Sat, 15 Jan 2022 20:30:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:49: #1 tags after filtering in treatment: 3557016 INFO @ Sat, 15 Jan 2022 20:30:49: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:30:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:30:49: #2 number of paired peaks: 31 WARNING @ Sat, 15 Jan 2022 20:30:49: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:30:55: 17000000 INFO @ Sat, 15 Jan 2022 20:30:57: #1 tag size is determined as 33 bps INFO @ Sat, 15 Jan 2022 20:30:57: #1 tag size = 33 INFO @ Sat, 15 Jan 2022 20:30:57: #1 total tags in treatment: 4262765 INFO @ Sat, 15 Jan 2022 20:30:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:57: #1 tags after filtering in treatment: 3557016 INFO @ Sat, 15 Jan 2022 20:30:57: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:30:57: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:30:58: #2 number of paired peaks: 31 WARNING @ Sat, 15 Jan 2022 20:30:58: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245326/SRX8245326.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling