Job ID = 14520919 SRX = SRX8245325 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10711897 spots for SRR11684520/SRR11684520.sra Written 10711897 spots for SRR11684520/SRR11684520.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:30 10711897 reads; of these: 10711897 (100.00%) were paired; of these: 5025921 (46.92%) aligned concordantly 0 times 4627222 (43.20%) aligned concordantly exactly 1 time 1058754 (9.88%) aligned concordantly >1 times ---- 5025921 pairs aligned concordantly 0 times; of these: 1756 (0.03%) aligned discordantly 1 time ---- 5024165 pairs aligned 0 times concordantly or discordantly; of these: 10048330 mates make up the pairs; of these: 6828381 (67.96%) aligned 0 times 2617592 (26.05%) aligned exactly 1 time 602357 (5.99%) aligned >1 times 68.13% overall alignment rate Time searching: 00:05:30 Overall time: 00:05:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 423505 / 5687008 = 0.0745 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:19:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:19:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:19:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:19:51: 1000000 INFO @ Sat, 15 Jan 2022 20:19:58: 2000000 INFO @ Sat, 15 Jan 2022 20:20:04: 3000000 INFO @ Sat, 15 Jan 2022 20:20:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:20:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:20:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:20:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:20:18: 5000000 INFO @ Sat, 15 Jan 2022 20:20:22: 1000000 INFO @ Sat, 15 Jan 2022 20:20:25: 6000000 INFO @ Sat, 15 Jan 2022 20:20:30: 2000000 INFO @ Sat, 15 Jan 2022 20:20:32: 7000000 INFO @ Sat, 15 Jan 2022 20:20:38: 3000000 INFO @ Sat, 15 Jan 2022 20:20:40: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:20:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:20:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:20:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:20:45: 4000000 INFO @ Sat, 15 Jan 2022 20:20:48: 9000000 INFO @ Sat, 15 Jan 2022 20:20:52: 1000000 INFO @ Sat, 15 Jan 2022 20:20:53: 5000000 INFO @ Sat, 15 Jan 2022 20:20:55: 10000000 INFO @ Sat, 15 Jan 2022 20:21:00: 2000000 INFO @ Sat, 15 Jan 2022 20:21:01: 6000000 INFO @ Sat, 15 Jan 2022 20:21:03: 11000000 INFO @ Sat, 15 Jan 2022 20:21:08: 3000000 INFO @ Sat, 15 Jan 2022 20:21:09: 7000000 INFO @ Sat, 15 Jan 2022 20:21:11: 12000000 INFO @ Sat, 15 Jan 2022 20:21:16: 4000000 INFO @ Sat, 15 Jan 2022 20:21:16: 8000000 INFO @ Sat, 15 Jan 2022 20:21:19: 13000000 INFO @ Sat, 15 Jan 2022 20:21:23: 5000000 INFO @ Sat, 15 Jan 2022 20:21:24: 9000000 INFO @ Sat, 15 Jan 2022 20:21:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:21:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:21:24: #1 total tags in treatment: 5262719 INFO @ Sat, 15 Jan 2022 20:21:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:21:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:21:24: #1 tags after filtering in treatment: 3682419 INFO @ Sat, 15 Jan 2022 20:21:24: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:21:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:21:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:21:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:21:25: #2 number of paired peaks: 36 WARNING @ Sat, 15 Jan 2022 20:21:25: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:21:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:21:31: 6000000 INFO @ Sat, 15 Jan 2022 20:21:32: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:21:38: 7000000 INFO @ Sat, 15 Jan 2022 20:21:39: 11000000 INFO @ Sat, 15 Jan 2022 20:21:45: 8000000 INFO @ Sat, 15 Jan 2022 20:21:47: 12000000 INFO @ Sat, 15 Jan 2022 20:21:53: 9000000 INFO @ Sat, 15 Jan 2022 20:21:55: 13000000 INFO @ Sat, 15 Jan 2022 20:22:00: 10000000 INFO @ Sat, 15 Jan 2022 20:22:00: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:22:00: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:22:00: #1 total tags in treatment: 5262719 INFO @ Sat, 15 Jan 2022 20:22:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:22:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:22:00: #1 tags after filtering in treatment: 3682419 INFO @ Sat, 15 Jan 2022 20:22:00: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:22:00: #1 finished! INFO @ Sat, 15 Jan 2022 20:22:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:22:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:22:00: #2 number of paired peaks: 36 WARNING @ Sat, 15 Jan 2022 20:22:00: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:22:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:22:07: 11000000 INFO @ Sat, 15 Jan 2022 20:22:13: 12000000 INFO @ Sat, 15 Jan 2022 20:22:19: 13000000 INFO @ Sat, 15 Jan 2022 20:22:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:22:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:22:24: #1 total tags in treatment: 5262719 INFO @ Sat, 15 Jan 2022 20:22:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:22:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:22:24: #1 tags after filtering in treatment: 3682419 INFO @ Sat, 15 Jan 2022 20:22:24: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:22:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:22:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:22:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:22:24: #2 number of paired peaks: 36 WARNING @ Sat, 15 Jan 2022 20:22:24: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:22:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245325/SRX8245325.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling