Job ID = 14520913 SRX = SRX8245320 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16591461 spots for SRR11684515/SRR11684515.sra Written 16591461 spots for SRR11684515/SRR11684515.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:25 16591461 reads; of these: 16591461 (100.00%) were paired; of these: 11676873 (70.38%) aligned concordantly 0 times 4335458 (26.13%) aligned concordantly exactly 1 time 579130 (3.49%) aligned concordantly >1 times ---- 11676873 pairs aligned concordantly 0 times; of these: 131087 (1.12%) aligned discordantly 1 time ---- 11545786 pairs aligned 0 times concordantly or discordantly; of these: 23091572 mates make up the pairs; of these: 12152582 (52.63%) aligned 0 times 9527484 (41.26%) aligned exactly 1 time 1411506 (6.11%) aligned >1 times 63.38% overall alignment rate Time searching: 00:10:25 Overall time: 00:10:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 135017 / 5045046 = 0.0268 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:26: 1000000 INFO @ Sat, 15 Jan 2022 20:28:30: 2000000 INFO @ Sat, 15 Jan 2022 20:28:34: 3000000 INFO @ Sat, 15 Jan 2022 20:28:38: 4000000 INFO @ Sat, 15 Jan 2022 20:28:43: 5000000 INFO @ Sat, 15 Jan 2022 20:28:47: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:52: 7000000 INFO @ Sat, 15 Jan 2022 20:28:56: 1000000 INFO @ Sat, 15 Jan 2022 20:28:56: 8000000 INFO @ Sat, 15 Jan 2022 20:29:01: 2000000 INFO @ Sat, 15 Jan 2022 20:29:01: 9000000 INFO @ Sat, 15 Jan 2022 20:29:05: 3000000 INFO @ Sat, 15 Jan 2022 20:29:05: 10000000 INFO @ Sat, 15 Jan 2022 20:29:10: 11000000 INFO @ Sat, 15 Jan 2022 20:29:10: 4000000 INFO @ Sat, 15 Jan 2022 20:29:15: 12000000 INFO @ Sat, 15 Jan 2022 20:29:15: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:29:19: 13000000 INFO @ Sat, 15 Jan 2022 20:29:20: 6000000 INFO @ Sat, 15 Jan 2022 20:29:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:29:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:29:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:29:24: 14000000 INFO @ Sat, 15 Jan 2022 20:29:25: 7000000 INFO @ Sat, 15 Jan 2022 20:29:27: 1000000 INFO @ Sat, 15 Jan 2022 20:29:29: 15000000 INFO @ Sat, 15 Jan 2022 20:29:30: 8000000 INFO @ Sat, 15 Jan 2022 20:29:32: 2000000 INFO @ Sat, 15 Jan 2022 20:29:34: 16000000 INFO @ Sat, 15 Jan 2022 20:29:35: 9000000 INFO @ Sat, 15 Jan 2022 20:29:37: 3000000 INFO @ Sat, 15 Jan 2022 20:29:39: 17000000 INFO @ Sat, 15 Jan 2022 20:29:40: 10000000 INFO @ Sat, 15 Jan 2022 20:29:42: 4000000 INFO @ Sat, 15 Jan 2022 20:29:45: 18000000 INFO @ Sat, 15 Jan 2022 20:29:45: 11000000 INFO @ Sat, 15 Jan 2022 20:29:47: 5000000 INFO @ Sat, 15 Jan 2022 20:29:50: 19000000 INFO @ Sat, 15 Jan 2022 20:29:50: 12000000 INFO @ Sat, 15 Jan 2022 20:29:52: 6000000 INFO @ Sat, 15 Jan 2022 20:29:55: 20000000 INFO @ Sat, 15 Jan 2022 20:29:56: 13000000 INFO @ Sat, 15 Jan 2022 20:29:58: 7000000 INFO @ Sat, 15 Jan 2022 20:29:59: #1 tag size is determined as 26 bps INFO @ Sat, 15 Jan 2022 20:29:59: #1 tag size = 26 INFO @ Sat, 15 Jan 2022 20:29:59: #1 total tags in treatment: 4780915 INFO @ Sat, 15 Jan 2022 20:29:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:29:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:29:59: #1 tags after filtering in treatment: 3967872 INFO @ Sat, 15 Jan 2022 20:29:59: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:29:59: #1 finished! INFO @ Sat, 15 Jan 2022 20:29:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:29:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:29:59: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 20:29:59: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:29:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:30:01: 14000000 INFO @ Sat, 15 Jan 2022 20:30:03: 8000000 INFO @ Sat, 15 Jan 2022 20:30:06: 15000000 INFO @ Sat, 15 Jan 2022 20:30:08: 9000000 INFO @ Sat, 15 Jan 2022 20:30:11: 16000000 INFO @ Sat, 15 Jan 2022 20:30:12: 10000000 INFO @ Sat, 15 Jan 2022 20:30:16: 17000000 INFO @ Sat, 15 Jan 2022 20:30:17: 11000000 INFO @ Sat, 15 Jan 2022 20:30:21: 18000000 INFO @ Sat, 15 Jan 2022 20:30:23: 12000000 INFO @ Sat, 15 Jan 2022 20:30:26: 19000000 INFO @ Sat, 15 Jan 2022 20:30:28: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:30:32: 20000000 INFO @ Sat, 15 Jan 2022 20:30:33: 14000000 INFO @ Sat, 15 Jan 2022 20:30:35: #1 tag size is determined as 26 bps INFO @ Sat, 15 Jan 2022 20:30:35: #1 tag size = 26 INFO @ Sat, 15 Jan 2022 20:30:35: #1 total tags in treatment: 4780915 INFO @ Sat, 15 Jan 2022 20:30:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:35: #1 tags after filtering in treatment: 3967872 INFO @ Sat, 15 Jan 2022 20:30:35: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:30:35: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:30:36: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 20:30:36: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:30:37: 15000000 INFO @ Sat, 15 Jan 2022 20:30:42: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:30:46: 17000000 INFO @ Sat, 15 Jan 2022 20:30:51: 18000000 INFO @ Sat, 15 Jan 2022 20:30:56: 19000000 INFO @ Sat, 15 Jan 2022 20:31:00: 20000000 INFO @ Sat, 15 Jan 2022 20:31:03: #1 tag size is determined as 26 bps INFO @ Sat, 15 Jan 2022 20:31:03: #1 tag size = 26 INFO @ Sat, 15 Jan 2022 20:31:03: #1 total tags in treatment: 4780915 INFO @ Sat, 15 Jan 2022 20:31:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:31:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:31:03: #1 tags after filtering in treatment: 3967872 INFO @ Sat, 15 Jan 2022 20:31:03: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:31:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:31:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:31:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:31:03: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 20:31:03: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:31:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245320/SRX8245320.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling