Job ID = 14520878 SRX = SRX8245316 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12963079 spots for SRR11684543/SRR11684543.sra Written 12963079 spots for SRR11684543/SRR11684543.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:39 12963079 reads; of these: 12963079 (100.00%) were paired; of these: 5545433 (42.78%) aligned concordantly 0 times 6462424 (49.85%) aligned concordantly exactly 1 time 955222 (7.37%) aligned concordantly >1 times ---- 5545433 pairs aligned concordantly 0 times; of these: 63224 (1.14%) aligned discordantly 1 time ---- 5482209 pairs aligned 0 times concordantly or discordantly; of these: 10964418 mates make up the pairs; of these: 6266294 (57.15%) aligned 0 times 4048537 (36.92%) aligned exactly 1 time 649587 (5.92%) aligned >1 times 75.83% overall alignment rate Time searching: 00:06:39 Overall time: 00:06:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 145738 / 7480023 = 0.0195 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:50: 1000000 INFO @ Sat, 15 Jan 2022 20:15:54: 2000000 INFO @ Sat, 15 Jan 2022 20:15:59: 3000000 INFO @ Sat, 15 Jan 2022 20:16:04: 4000000 INFO @ Sat, 15 Jan 2022 20:16:09: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:16:13: 6000000 INFO @ Sat, 15 Jan 2022 20:16:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:16:19: 7000000 INFO @ Sat, 15 Jan 2022 20:16:20: 1000000 INFO @ Sat, 15 Jan 2022 20:16:24: 8000000 INFO @ Sat, 15 Jan 2022 20:16:26: 2000000 INFO @ Sat, 15 Jan 2022 20:16:30: 9000000 INFO @ Sat, 15 Jan 2022 20:16:32: 3000000 INFO @ Sat, 15 Jan 2022 20:16:35: 10000000 INFO @ Sat, 15 Jan 2022 20:16:37: 4000000 INFO @ Sat, 15 Jan 2022 20:16:41: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:16:43: 5000000 INFO @ Sat, 15 Jan 2022 20:16:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:16:46: 12000000 INFO @ Sat, 15 Jan 2022 20:16:49: 6000000 INFO @ Sat, 15 Jan 2022 20:16:50: 1000000 INFO @ Sat, 15 Jan 2022 20:16:54: 13000000 INFO @ Sat, 15 Jan 2022 20:16:54: 7000000 INFO @ Sat, 15 Jan 2022 20:16:56: 2000000 INFO @ Sat, 15 Jan 2022 20:17:01: 8000000 INFO @ Sat, 15 Jan 2022 20:17:01: 14000000 INFO @ Sat, 15 Jan 2022 20:17:03: 3000000 INFO @ Sat, 15 Jan 2022 20:17:07: 15000000 INFO @ Sat, 15 Jan 2022 20:17:07: 9000000 INFO @ Sat, 15 Jan 2022 20:17:08: 4000000 INFO @ Sat, 15 Jan 2022 20:17:14: 16000000 INFO @ Sat, 15 Jan 2022 20:17:14: 10000000 INFO @ Sat, 15 Jan 2022 20:17:14: 5000000 INFO @ Sat, 15 Jan 2022 20:17:22: 11000000 INFO @ Sat, 15 Jan 2022 20:17:22: 17000000 INFO @ Sat, 15 Jan 2022 20:17:22: 6000000 INFO @ Sat, 15 Jan 2022 20:17:28: 12000000 INFO @ Sat, 15 Jan 2022 20:17:28: 7000000 INFO @ Sat, 15 Jan 2022 20:17:29: 18000000 INFO @ Sat, 15 Jan 2022 20:17:34: 13000000 INFO @ Sat, 15 Jan 2022 20:17:35: 8000000 INFO @ Sat, 15 Jan 2022 20:17:35: 19000000 INFO @ Sat, 15 Jan 2022 20:17:37: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:17:37: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:17:37: #1 total tags in treatment: 7272038 INFO @ Sat, 15 Jan 2022 20:17:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:37: #1 tags after filtering in treatment: 5522456 INFO @ Sat, 15 Jan 2022 20:17:37: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:17:37: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:17:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 120 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:17:40: 14000000 INFO @ Sat, 15 Jan 2022 20:17:41: 9000000 INFO @ Sat, 15 Jan 2022 20:17:45: 15000000 INFO @ Sat, 15 Jan 2022 20:17:47: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:17:51: 16000000 INFO @ Sat, 15 Jan 2022 20:17:53: 11000000 INFO @ Sat, 15 Jan 2022 20:17:57: 17000000 INFO @ Sat, 15 Jan 2022 20:17:59: 12000000 INFO @ Sat, 15 Jan 2022 20:18:02: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:18:06: 13000000 INFO @ Sat, 15 Jan 2022 20:18:08: 19000000 INFO @ Sat, 15 Jan 2022 20:18:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:18:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:18:10: #1 total tags in treatment: 7272038 INFO @ Sat, 15 Jan 2022 20:18:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:18:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:18:10: #1 tags after filtering in treatment: 5522456 INFO @ Sat, 15 Jan 2022 20:18:10: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:18:10: #1 finished! INFO @ Sat, 15 Jan 2022 20:18:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:18:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:18:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:18:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:18:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:18:12: 14000000 INFO @ Sat, 15 Jan 2022 20:18:17: 15000000 INFO @ Sat, 15 Jan 2022 20:18:23: 16000000 INFO @ Sat, 15 Jan 2022 20:18:29: 17000000 INFO @ Sat, 15 Jan 2022 20:18:35: 18000000 INFO @ Sat, 15 Jan 2022 20:18:40: 19000000 INFO @ Sat, 15 Jan 2022 20:18:42: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:18:42: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:18:42: #1 total tags in treatment: 7272038 INFO @ Sat, 15 Jan 2022 20:18:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:18:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:18:42: #1 tags after filtering in treatment: 5522456 INFO @ Sat, 15 Jan 2022 20:18:42: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:18:42: #1 finished! INFO @ Sat, 15 Jan 2022 20:18:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:18:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:18:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:18:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:18:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245316/SRX8245316.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling