Job ID = 14520877 SRX = SRX8245315 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13040932 spots for SRR11684542/SRR11684542.sra Written 13040932 spots for SRR11684542/SRR11684542.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:54 13040932 reads; of these: 13040932 (100.00%) were paired; of these: 5448840 (41.78%) aligned concordantly 0 times 6526486 (50.05%) aligned concordantly exactly 1 time 1065606 (8.17%) aligned concordantly >1 times ---- 5448840 pairs aligned concordantly 0 times; of these: 32245 (0.59%) aligned discordantly 1 time ---- 5416595 pairs aligned 0 times concordantly or discordantly; of these: 10833190 mates make up the pairs; of these: 6132002 (56.60%) aligned 0 times 4014699 (37.06%) aligned exactly 1 time 686489 (6.34%) aligned >1 times 76.49% overall alignment rate Time searching: 00:11:54 Overall time: 00:11:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 181655 / 7623338 = 0.0238 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:25:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:25:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:25:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:25:55: 1000000 INFO @ Sat, 15 Jan 2022 20:26:02: 2000000 INFO @ Sat, 15 Jan 2022 20:26:10: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:18: 4000000 INFO @ Sat, 15 Jan 2022 20:26:25: 1000000 INFO @ Sat, 15 Jan 2022 20:26:26: 5000000 INFO @ Sat, 15 Jan 2022 20:26:34: 2000000 INFO @ Sat, 15 Jan 2022 20:26:35: 6000000 INFO @ Sat, 15 Jan 2022 20:26:42: 3000000 INFO @ Sat, 15 Jan 2022 20:26:44: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:50: 4000000 INFO @ Sat, 15 Jan 2022 20:26:52: 8000000 INFO @ Sat, 15 Jan 2022 20:26:55: 1000000 INFO @ Sat, 15 Jan 2022 20:26:59: 5000000 INFO @ Sat, 15 Jan 2022 20:27:01: 9000000 INFO @ Sat, 15 Jan 2022 20:27:04: 2000000 INFO @ Sat, 15 Jan 2022 20:27:07: 6000000 INFO @ Sat, 15 Jan 2022 20:27:09: 10000000 INFO @ Sat, 15 Jan 2022 20:27:12: 3000000 INFO @ Sat, 15 Jan 2022 20:27:16: 7000000 INFO @ Sat, 15 Jan 2022 20:27:19: 11000000 INFO @ Sat, 15 Jan 2022 20:27:21: 4000000 INFO @ Sat, 15 Jan 2022 20:27:25: 8000000 INFO @ Sat, 15 Jan 2022 20:27:27: 12000000 INFO @ Sat, 15 Jan 2022 20:27:30: 5000000 INFO @ Sat, 15 Jan 2022 20:27:33: 9000000 INFO @ Sat, 15 Jan 2022 20:27:35: 13000000 INFO @ Sat, 15 Jan 2022 20:27:38: 6000000 INFO @ Sat, 15 Jan 2022 20:27:42: 10000000 INFO @ Sat, 15 Jan 2022 20:27:44: 14000000 INFO @ Sat, 15 Jan 2022 20:27:47: 7000000 INFO @ Sat, 15 Jan 2022 20:27:50: 11000000 INFO @ Sat, 15 Jan 2022 20:27:52: 15000000 INFO @ Sat, 15 Jan 2022 20:27:55: 8000000 INFO @ Sat, 15 Jan 2022 20:27:59: 12000000 INFO @ Sat, 15 Jan 2022 20:28:01: 16000000 INFO @ Sat, 15 Jan 2022 20:28:04: 9000000 INFO @ Sat, 15 Jan 2022 20:28:08: 13000000 INFO @ Sat, 15 Jan 2022 20:28:10: 17000000 INFO @ Sat, 15 Jan 2022 20:28:13: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:28:16: 14000000 INFO @ Sat, 15 Jan 2022 20:28:18: 18000000 INFO @ Sat, 15 Jan 2022 20:28:21: 11000000 INFO @ Sat, 15 Jan 2022 20:28:24: 15000000 INFO @ Sat, 15 Jan 2022 20:28:27: 19000000 INFO @ Sat, 15 Jan 2022 20:28:29: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:28:32: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:28:32: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:28:32: #1 total tags in treatment: 7410503 INFO @ Sat, 15 Jan 2022 20:28:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:28:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:28:32: #1 tags after filtering in treatment: 5660113 INFO @ Sat, 15 Jan 2022 20:28:32: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:28:32: #1 finished! INFO @ Sat, 15 Jan 2022 20:28:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:28:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:28:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:28:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:28:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:28:33: 16000000 INFO @ Sat, 15 Jan 2022 20:28:38: 13000000 INFO @ Sat, 15 Jan 2022 20:28:41: 17000000 INFO @ Sat, 15 Jan 2022 20:28:46: 14000000 INFO @ Sat, 15 Jan 2022 20:28:50: 18000000 INFO @ Sat, 15 Jan 2022 20:28:54: 15000000 INFO @ Sat, 15 Jan 2022 20:28:58: 19000000 INFO @ Sat, 15 Jan 2022 20:29:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:29:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:29:03: #1 total tags in treatment: 7410503 INFO @ Sat, 15 Jan 2022 20:29:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:29:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:29:03: 16000000 INFO @ Sat, 15 Jan 2022 20:29:03: #1 tags after filtering in treatment: 5660113 INFO @ Sat, 15 Jan 2022 20:29:03: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:29:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:29:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:29:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:29:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:29:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:29:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:29:12: 17000000 INFO @ Sat, 15 Jan 2022 20:29:21: 18000000 INFO @ Sat, 15 Jan 2022 20:29:29: 19000000 INFO @ Sat, 15 Jan 2022 20:29:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:29:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:29:34: #1 total tags in treatment: 7410503 INFO @ Sat, 15 Jan 2022 20:29:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:29:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:29:34: #1 tags after filtering in treatment: 5660113 INFO @ Sat, 15 Jan 2022 20:29:34: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:29:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:29:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:29:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:29:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:29:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:29:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245315/SRX8245315.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling