Job ID = 14520876 SRX = SRX8245314 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13191990 spots for SRR11684541/SRR11684541.sra Written 13191990 spots for SRR11684541/SRR11684541.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:21 13191990 reads; of these: 13191990 (100.00%) were paired; of these: 6167996 (46.76%) aligned concordantly 0 times 6146419 (46.59%) aligned concordantly exactly 1 time 877575 (6.65%) aligned concordantly >1 times ---- 6167996 pairs aligned concordantly 0 times; of these: 55613 (0.90%) aligned discordantly 1 time ---- 6112383 pairs aligned 0 times concordantly or discordantly; of these: 12224766 mates make up the pairs; of these: 6856811 (56.09%) aligned 0 times 4654774 (38.08%) aligned exactly 1 time 713181 (5.83%) aligned >1 times 74.01% overall alignment rate Time searching: 00:07:21 Overall time: 00:07:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 125380 / 7078684 = 0.0177 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:17:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:17:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:17:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:17:35: 1000000 INFO @ Sat, 15 Jan 2022 20:17:41: 2000000 INFO @ Sat, 15 Jan 2022 20:17:46: 3000000 INFO @ Sat, 15 Jan 2022 20:17:52: 4000000 INFO @ Sat, 15 Jan 2022 20:17:57: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:18:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:18:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:18:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:18:03: 6000000 INFO @ Sat, 15 Jan 2022 20:18:05: 1000000 INFO @ Sat, 15 Jan 2022 20:18:09: 7000000 INFO @ Sat, 15 Jan 2022 20:18:10: 2000000 INFO @ Sat, 15 Jan 2022 20:18:14: 8000000 INFO @ Sat, 15 Jan 2022 20:18:15: 3000000 INFO @ Sat, 15 Jan 2022 20:18:20: 4000000 INFO @ Sat, 15 Jan 2022 20:18:20: 9000000 INFO @ Sat, 15 Jan 2022 20:18:25: 5000000 INFO @ Sat, 15 Jan 2022 20:18:26: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:18:30: 6000000 INFO @ Sat, 15 Jan 2022 20:18:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:18:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:18:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:18:32: 11000000 INFO @ Sat, 15 Jan 2022 20:18:35: 7000000 INFO @ Sat, 15 Jan 2022 20:18:36: 1000000 INFO @ Sat, 15 Jan 2022 20:18:37: 12000000 INFO @ Sat, 15 Jan 2022 20:18:40: 8000000 INFO @ Sat, 15 Jan 2022 20:18:42: 2000000 INFO @ Sat, 15 Jan 2022 20:18:43: 13000000 INFO @ Sat, 15 Jan 2022 20:18:45: 9000000 INFO @ Sat, 15 Jan 2022 20:18:47: 3000000 INFO @ Sat, 15 Jan 2022 20:18:49: 14000000 INFO @ Sat, 15 Jan 2022 20:18:50: 10000000 INFO @ Sat, 15 Jan 2022 20:18:53: 4000000 INFO @ Sat, 15 Jan 2022 20:18:55: 15000000 INFO @ Sat, 15 Jan 2022 20:18:55: 11000000 INFO @ Sat, 15 Jan 2022 20:18:59: 5000000 INFO @ Sat, 15 Jan 2022 20:19:00: 12000000 INFO @ Sat, 15 Jan 2022 20:19:01: 16000000 INFO @ Sat, 15 Jan 2022 20:19:05: 6000000 INFO @ Sat, 15 Jan 2022 20:19:05: 13000000 INFO @ Sat, 15 Jan 2022 20:19:06: 17000000 INFO @ Sat, 15 Jan 2022 20:19:10: 14000000 INFO @ Sat, 15 Jan 2022 20:19:10: 7000000 INFO @ Sat, 15 Jan 2022 20:19:12: 18000000 INFO @ Sat, 15 Jan 2022 20:19:15: 15000000 INFO @ Sat, 15 Jan 2022 20:19:16: 8000000 INFO @ Sat, 15 Jan 2022 20:19:18: 19000000 INFO @ Sat, 15 Jan 2022 20:19:20: 16000000 INFO @ Sat, 15 Jan 2022 20:19:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:19:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:19:20: #1 total tags in treatment: 6898740 INFO @ Sat, 15 Jan 2022 20:19:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:19:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:19:20: #1 tags after filtering in treatment: 5337195 INFO @ Sat, 15 Jan 2022 20:19:20: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:19:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:19:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:19:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:19:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:19:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:19:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:19:22: 9000000 INFO @ Sat, 15 Jan 2022 20:19:25: 17000000 INFO @ Sat, 15 Jan 2022 20:19:27: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:19:30: 18000000 INFO @ Sat, 15 Jan 2022 20:19:33: 11000000 INFO @ Sat, 15 Jan 2022 20:19:35: 19000000 INFO @ Sat, 15 Jan 2022 20:19:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:19:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:19:36: #1 total tags in treatment: 6898740 INFO @ Sat, 15 Jan 2022 20:19:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:19:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:19:36: #1 tags after filtering in treatment: 5337195 INFO @ Sat, 15 Jan 2022 20:19:36: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:19:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:19:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:19:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:19:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:19:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:19:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:19:38: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:19:44: 13000000 INFO @ Sat, 15 Jan 2022 20:19:49: 14000000 INFO @ Sat, 15 Jan 2022 20:19:54: 15000000 INFO @ Sat, 15 Jan 2022 20:20:00: 16000000 INFO @ Sat, 15 Jan 2022 20:20:05: 17000000 INFO @ Sat, 15 Jan 2022 20:20:11: 18000000 INFO @ Sat, 15 Jan 2022 20:20:16: 19000000 INFO @ Sat, 15 Jan 2022 20:20:18: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:20:18: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:20:18: #1 total tags in treatment: 6898740 INFO @ Sat, 15 Jan 2022 20:20:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:20:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:20:18: #1 tags after filtering in treatment: 5337195 INFO @ Sat, 15 Jan 2022 20:20:18: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:20:18: #1 finished! INFO @ Sat, 15 Jan 2022 20:20:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:20:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:20:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:20:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:20:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245314/SRX8245314.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling