Job ID = 14520875 SRX = SRX8245313 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15624381 spots for SRR11684540/SRR11684540.sra Written 15624381 spots for SRR11684540/SRR11684540.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:42 15624381 reads; of these: 15624381 (100.00%) were paired; of these: 7391064 (47.30%) aligned concordantly 0 times 7143725 (45.72%) aligned concordantly exactly 1 time 1089592 (6.97%) aligned concordantly >1 times ---- 7391064 pairs aligned concordantly 0 times; of these: 47536 (0.64%) aligned discordantly 1 time ---- 7343528 pairs aligned 0 times concordantly or discordantly; of these: 14687056 mates make up the pairs; of these: 8258090 (56.23%) aligned 0 times 5542147 (37.73%) aligned exactly 1 time 886819 (6.04%) aligned >1 times 73.57% overall alignment rate Time searching: 00:12:42 Overall time: 00:12:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 198784 / 8279918 = 0.0240 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:08: 1000000 INFO @ Sat, 15 Jan 2022 20:26:14: 2000000 INFO @ Sat, 15 Jan 2022 20:26:20: 3000000 INFO @ Sat, 15 Jan 2022 20:26:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:32: 5000000 INFO @ Sat, 15 Jan 2022 20:26:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:38: 6000000 INFO @ Sat, 15 Jan 2022 20:26:41: 1000000 INFO @ Sat, 15 Jan 2022 20:26:44: 7000000 INFO @ Sat, 15 Jan 2022 20:26:49: 2000000 INFO @ Sat, 15 Jan 2022 20:26:50: 8000000 INFO @ Sat, 15 Jan 2022 20:26:56: 9000000 INFO @ Sat, 15 Jan 2022 20:26:57: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:27:02: 10000000 INFO @ Sat, 15 Jan 2022 20:27:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:27:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:27:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:27:05: 4000000 INFO @ Sat, 15 Jan 2022 20:27:08: 11000000 INFO @ Sat, 15 Jan 2022 20:27:12: 1000000 INFO @ Sat, 15 Jan 2022 20:27:14: 5000000 INFO @ Sat, 15 Jan 2022 20:27:14: 12000000 INFO @ Sat, 15 Jan 2022 20:27:20: 13000000 INFO @ Sat, 15 Jan 2022 20:27:21: 2000000 INFO @ Sat, 15 Jan 2022 20:27:23: 6000000 INFO @ Sat, 15 Jan 2022 20:27:26: 14000000 INFO @ Sat, 15 Jan 2022 20:27:29: 3000000 INFO @ Sat, 15 Jan 2022 20:27:31: 7000000 INFO @ Sat, 15 Jan 2022 20:27:32: 15000000 INFO @ Sat, 15 Jan 2022 20:27:38: 4000000 INFO @ Sat, 15 Jan 2022 20:27:38: 16000000 INFO @ Sat, 15 Jan 2022 20:27:40: 8000000 INFO @ Sat, 15 Jan 2022 20:27:45: 17000000 INFO @ Sat, 15 Jan 2022 20:27:47: 5000000 INFO @ Sat, 15 Jan 2022 20:27:48: 9000000 INFO @ Sat, 15 Jan 2022 20:27:51: 18000000 INFO @ Sat, 15 Jan 2022 20:27:55: 6000000 INFO @ Sat, 15 Jan 2022 20:27:57: 19000000 INFO @ Sat, 15 Jan 2022 20:27:57: 10000000 INFO @ Sat, 15 Jan 2022 20:28:03: 20000000 INFO @ Sat, 15 Jan 2022 20:28:04: 7000000 INFO @ Sat, 15 Jan 2022 20:28:06: 11000000 INFO @ Sat, 15 Jan 2022 20:28:09: 21000000 INFO @ Sat, 15 Jan 2022 20:28:13: 8000000 INFO @ Sat, 15 Jan 2022 20:28:14: 12000000 INFO @ Sat, 15 Jan 2022 20:28:15: 22000000 INFO @ Sat, 15 Jan 2022 20:28:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:28:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:28:19: #1 total tags in treatment: 8034633 INFO @ Sat, 15 Jan 2022 20:28:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:28:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:28:19: #1 tags after filtering in treatment: 5970406 INFO @ Sat, 15 Jan 2022 20:28:19: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 20:28:19: #1 finished! INFO @ Sat, 15 Jan 2022 20:28:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:28:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:28:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:28:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:28:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:28:21: 9000000 INFO @ Sat, 15 Jan 2022 20:28:23: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:28:30: 10000000 INFO @ Sat, 15 Jan 2022 20:28:32: 14000000 INFO @ Sat, 15 Jan 2022 20:28:38: 11000000 INFO @ Sat, 15 Jan 2022 20:28:40: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:28:47: 12000000 INFO @ Sat, 15 Jan 2022 20:28:48: 16000000 INFO @ Sat, 15 Jan 2022 20:28:55: 13000000 INFO @ Sat, 15 Jan 2022 20:28:57: 17000000 INFO @ Sat, 15 Jan 2022 20:29:03: 14000000 INFO @ Sat, 15 Jan 2022 20:29:05: 18000000 INFO @ Sat, 15 Jan 2022 20:29:12: 15000000 INFO @ Sat, 15 Jan 2022 20:29:13: 19000000 INFO @ Sat, 15 Jan 2022 20:29:20: 16000000 INFO @ Sat, 15 Jan 2022 20:29:22: 20000000 INFO @ Sat, 15 Jan 2022 20:29:29: 17000000 INFO @ Sat, 15 Jan 2022 20:29:30: 21000000 INFO @ Sat, 15 Jan 2022 20:29:37: 18000000 INFO @ Sat, 15 Jan 2022 20:29:39: 22000000 INFO @ Sat, 15 Jan 2022 20:29:44: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:29:44: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:29:44: #1 total tags in treatment: 8034633 INFO @ Sat, 15 Jan 2022 20:29:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:29:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:29:44: #1 tags after filtering in treatment: 5970406 INFO @ Sat, 15 Jan 2022 20:29:44: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 20:29:44: #1 finished! INFO @ Sat, 15 Jan 2022 20:29:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:29:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:29:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:29:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:29:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:29:46: 19000000 INFO @ Sat, 15 Jan 2022 20:29:54: 20000000 INFO @ Sat, 15 Jan 2022 20:30:02: 21000000 INFO @ Sat, 15 Jan 2022 20:30:10: 22000000 INFO @ Sat, 15 Jan 2022 20:30:15: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:30:15: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:30:15: #1 total tags in treatment: 8034633 INFO @ Sat, 15 Jan 2022 20:30:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:15: #1 tags after filtering in treatment: 5970406 INFO @ Sat, 15 Jan 2022 20:30:15: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 20:30:15: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:30:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:30:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245313/SRX8245313.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling