Job ID = 14520840 SRX = SRX8245309 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12567011 spots for SRR11684536/SRR11684536.sra Written 12567011 spots for SRR11684536/SRR11684536.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:22 12567011 reads; of these: 12567011 (100.00%) were paired; of these: 5226252 (41.59%) aligned concordantly 0 times 6279543 (49.97%) aligned concordantly exactly 1 time 1061216 (8.44%) aligned concordantly >1 times ---- 5226252 pairs aligned concordantly 0 times; of these: 182775 (3.50%) aligned discordantly 1 time ---- 5043477 pairs aligned 0 times concordantly or discordantly; of these: 10086954 mates make up the pairs; of these: 5802874 (57.53%) aligned 0 times 3533771 (35.03%) aligned exactly 1 time 750309 (7.44%) aligned >1 times 76.91% overall alignment rate Time searching: 00:07:22 Overall time: 00:07:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 205706 / 7522342 = 0.0273 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:18:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:18:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:18:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:18:36: 1000000 INFO @ Sat, 15 Jan 2022 20:18:42: 2000000 INFO @ Sat, 15 Jan 2022 20:18:47: 3000000 INFO @ Sat, 15 Jan 2022 20:18:52: 4000000 INFO @ Sat, 15 Jan 2022 20:18:57: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:19:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:19:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:19:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:19:03: 6000000 INFO @ Sat, 15 Jan 2022 20:19:07: 1000000 INFO @ Sat, 15 Jan 2022 20:19:09: 7000000 INFO @ Sat, 15 Jan 2022 20:19:14: 2000000 INFO @ Sat, 15 Jan 2022 20:19:15: 8000000 INFO @ Sat, 15 Jan 2022 20:19:20: 3000000 INFO @ Sat, 15 Jan 2022 20:19:21: 9000000 INFO @ Sat, 15 Jan 2022 20:19:26: 4000000 INFO @ Sat, 15 Jan 2022 20:19:28: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:19:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:19:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:19:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:19:32: 5000000 INFO @ Sat, 15 Jan 2022 20:19:34: 11000000 INFO @ Sat, 15 Jan 2022 20:19:39: 1000000 INFO @ Sat, 15 Jan 2022 20:19:39: 6000000 INFO @ Sat, 15 Jan 2022 20:19:41: 12000000 INFO @ Sat, 15 Jan 2022 20:19:46: 7000000 INFO @ Sat, 15 Jan 2022 20:19:46: 2000000 INFO @ Sat, 15 Jan 2022 20:19:48: 13000000 INFO @ Sat, 15 Jan 2022 20:19:53: 8000000 INFO @ Sat, 15 Jan 2022 20:19:54: 3000000 INFO @ Sat, 15 Jan 2022 20:19:55: 14000000 INFO @ Sat, 15 Jan 2022 20:20:00: 9000000 INFO @ Sat, 15 Jan 2022 20:20:01: 4000000 INFO @ Sat, 15 Jan 2022 20:20:02: 15000000 INFO @ Sat, 15 Jan 2022 20:20:07: 10000000 INFO @ Sat, 15 Jan 2022 20:20:09: 16000000 INFO @ Sat, 15 Jan 2022 20:20:09: 5000000 INFO @ Sat, 15 Jan 2022 20:20:14: 11000000 INFO @ Sat, 15 Jan 2022 20:20:16: 17000000 INFO @ Sat, 15 Jan 2022 20:20:16: 6000000 INFO @ Sat, 15 Jan 2022 20:20:21: 12000000 INFO @ Sat, 15 Jan 2022 20:20:23: 18000000 INFO @ Sat, 15 Jan 2022 20:20:24: 7000000 INFO @ Sat, 15 Jan 2022 20:20:28: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:20:29: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:20:29: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:20:29: #1 total tags in treatment: 7136348 INFO @ Sat, 15 Jan 2022 20:20:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:20:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:20:29: #1 tags after filtering in treatment: 5339780 INFO @ Sat, 15 Jan 2022 20:20:29: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:20:29: #1 finished! INFO @ Sat, 15 Jan 2022 20:20:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:20:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:20:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:20:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:20:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:20:31: 8000000 INFO @ Sat, 15 Jan 2022 20:20:34: 14000000 INFO @ Sat, 15 Jan 2022 20:20:39: 9000000 INFO @ Sat, 15 Jan 2022 20:20:41: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:20:46: 10000000 INFO @ Sat, 15 Jan 2022 20:20:48: 16000000 INFO @ Sat, 15 Jan 2022 20:20:53: 11000000 INFO @ Sat, 15 Jan 2022 20:20:55: 17000000 INFO @ Sat, 15 Jan 2022 20:21:01: 12000000 INFO @ Sat, 15 Jan 2022 20:21:02: 18000000 INFO @ Sat, 15 Jan 2022 20:21:08: 13000000 INFO @ Sat, 15 Jan 2022 20:21:08: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:21:08: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:21:08: #1 total tags in treatment: 7136348 INFO @ Sat, 15 Jan 2022 20:21:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:21:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:21:09: #1 tags after filtering in treatment: 5339780 INFO @ Sat, 15 Jan 2022 20:21:09: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:21:09: #1 finished! INFO @ Sat, 15 Jan 2022 20:21:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:21:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:21:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:21:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:21:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:21:15: 14000000 INFO @ Sat, 15 Jan 2022 20:21:22: 15000000 INFO @ Sat, 15 Jan 2022 20:21:28: 16000000 INFO @ Sat, 15 Jan 2022 20:21:35: 17000000 INFO @ Sat, 15 Jan 2022 20:21:42: 18000000 INFO @ Sat, 15 Jan 2022 20:21:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:21:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:21:48: #1 total tags in treatment: 7136348 INFO @ Sat, 15 Jan 2022 20:21:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:21:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:21:48: #1 tags after filtering in treatment: 5339780 INFO @ Sat, 15 Jan 2022 20:21:48: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:21:48: #1 finished! INFO @ Sat, 15 Jan 2022 20:21:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:21:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:21:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:21:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:21:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245309/SRX8245309.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling