Job ID = 14520838 SRX = SRX8245307 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 6803262 spots for SRR11684534/SRR11684534.sra Written 6803262 spots for SRR11684534/SRR11684534.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:07 6803262 reads; of these: 6803262 (100.00%) were paired; of these: 2402067 (35.31%) aligned concordantly 0 times 3846725 (56.54%) aligned concordantly exactly 1 time 554470 (8.15%) aligned concordantly >1 times ---- 2402067 pairs aligned concordantly 0 times; of these: 259056 (10.78%) aligned discordantly 1 time ---- 2143011 pairs aligned 0 times concordantly or discordantly; of these: 4286022 mates make up the pairs; of these: 2512637 (58.62%) aligned 0 times 1455467 (33.96%) aligned exactly 1 time 317918 (7.42%) aligned >1 times 81.53% overall alignment rate Time searching: 00:04:07 Overall time: 00:04:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 58752 / 4659635 = 0.0126 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:07:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:07:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:07:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:08:00: 1000000 INFO @ Sat, 15 Jan 2022 20:08:07: 2000000 INFO @ Sat, 15 Jan 2022 20:08:13: 3000000 INFO @ Sat, 15 Jan 2022 20:08:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:08:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:08:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:08:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:08:27: 5000000 INFO @ Sat, 15 Jan 2022 20:08:31: 1000000 INFO @ Sat, 15 Jan 2022 20:08:34: 6000000 INFO @ Sat, 15 Jan 2022 20:08:37: 2000000 INFO @ Sat, 15 Jan 2022 20:08:41: 7000000 INFO @ Sat, 15 Jan 2022 20:08:44: 3000000 INFO @ Sat, 15 Jan 2022 20:08:49: 8000000 INFO @ Sat, 15 Jan 2022 20:08:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:08:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:08:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:08:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:08:56: 9000000 INFO @ Sat, 15 Jan 2022 20:08:56: 5000000 INFO @ Sat, 15 Jan 2022 20:09:01: 1000000 INFO @ Sat, 15 Jan 2022 20:09:03: 6000000 INFO @ Sat, 15 Jan 2022 20:09:03: 10000000 INFO @ Sat, 15 Jan 2022 20:09:07: 2000000 INFO @ Sat, 15 Jan 2022 20:09:09: 7000000 INFO @ Sat, 15 Jan 2022 20:09:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:09:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:09:10: #1 total tags in treatment: 4344043 INFO @ Sat, 15 Jan 2022 20:09:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:10: #1 tags after filtering in treatment: 3546626 INFO @ Sat, 15 Jan 2022 20:09:10: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:09:10: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:11: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:09:11: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:09:13: 3000000 INFO @ Sat, 15 Jan 2022 20:09:16: 8000000 INFO @ Sat, 15 Jan 2022 20:09:20: 4000000 INFO @ Sat, 15 Jan 2022 20:09:22: 9000000 INFO @ Sat, 15 Jan 2022 20:09:26: 5000000 INFO @ Sat, 15 Jan 2022 20:09:28: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:09:32: 6000000 INFO @ Sat, 15 Jan 2022 20:09:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:09:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:09:34: #1 total tags in treatment: 4344043 INFO @ Sat, 15 Jan 2022 20:09:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:34: #1 tags after filtering in treatment: 3546626 INFO @ Sat, 15 Jan 2022 20:09:34: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:09:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:35: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:09:35: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 385 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:09:38: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:09:43: 8000000 INFO @ Sat, 15 Jan 2022 20:09:48: 9000000 INFO @ Sat, 15 Jan 2022 20:09:53: 10000000 INFO @ Sat, 15 Jan 2022 20:09:58: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:09:58: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:09:58: #1 total tags in treatment: 4344043 INFO @ Sat, 15 Jan 2022 20:09:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:58: #1 tags after filtering in treatment: 3546626 INFO @ Sat, 15 Jan 2022 20:09:58: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:09:58: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:59: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:09:59: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245307/SRX8245307.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling