Job ID = 14520834 SRX = SRX8245303 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18709925 spots for SRR11684530/SRR11684530.sra Written 18709925 spots for SRR11684530/SRR11684530.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:40 18709925 reads; of these: 18709925 (100.00%) were paired; of these: 12573476 (67.20%) aligned concordantly 0 times 5268025 (28.16%) aligned concordantly exactly 1 time 868424 (4.64%) aligned concordantly >1 times ---- 12573476 pairs aligned concordantly 0 times; of these: 179653 (1.43%) aligned discordantly 1 time ---- 12393823 pairs aligned 0 times concordantly or discordantly; of these: 24787646 mates make up the pairs; of these: 12617901 (50.90%) aligned 0 times 10338542 (41.71%) aligned exactly 1 time 1831203 (7.39%) aligned >1 times 66.28% overall alignment rate Time searching: 00:08:40 Overall time: 00:08:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 188823 / 6315472 = 0.0299 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:14:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:14:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:14:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:14:39: 1000000 INFO @ Sat, 15 Jan 2022 20:14:42: 2000000 INFO @ Sat, 15 Jan 2022 20:14:46: 3000000 INFO @ Sat, 15 Jan 2022 20:14:50: 4000000 INFO @ Sat, 15 Jan 2022 20:14:54: 5000000 INFO @ Sat, 15 Jan 2022 20:14:58: 6000000 INFO @ Sat, 15 Jan 2022 20:15:01: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:05: 8000000 INFO @ Sat, 15 Jan 2022 20:15:09: 1000000 INFO @ Sat, 15 Jan 2022 20:15:09: 9000000 INFO @ Sat, 15 Jan 2022 20:15:13: 2000000 INFO @ Sat, 15 Jan 2022 20:15:13: 10000000 INFO @ Sat, 15 Jan 2022 20:15:17: 3000000 INFO @ Sat, 15 Jan 2022 20:15:17: 11000000 INFO @ Sat, 15 Jan 2022 20:15:21: 4000000 INFO @ Sat, 15 Jan 2022 20:15:21: 12000000 INFO @ Sat, 15 Jan 2022 20:15:25: 5000000 INFO @ Sat, 15 Jan 2022 20:15:25: 13000000 INFO @ Sat, 15 Jan 2022 20:15:29: 6000000 INFO @ Sat, 15 Jan 2022 20:15:29: 14000000 INFO @ Sat, 15 Jan 2022 20:15:33: 7000000 INFO @ Sat, 15 Jan 2022 20:15:33: 15000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:37: 8000000 INFO @ Sat, 15 Jan 2022 20:15:37: 16000000 INFO @ Sat, 15 Jan 2022 20:15:40: 1000000 INFO @ Sat, 15 Jan 2022 20:15:41: 9000000 INFO @ Sat, 15 Jan 2022 20:15:41: 17000000 INFO @ Sat, 15 Jan 2022 20:15:44: 2000000 INFO @ Sat, 15 Jan 2022 20:15:45: 10000000 INFO @ Sat, 15 Jan 2022 20:15:45: 18000000 INFO @ Sat, 15 Jan 2022 20:15:49: 11000000 INFO @ Sat, 15 Jan 2022 20:15:49: 19000000 INFO @ Sat, 15 Jan 2022 20:15:49: 3000000 INFO @ Sat, 15 Jan 2022 20:15:53: 12000000 INFO @ Sat, 15 Jan 2022 20:15:53: 20000000 INFO @ Sat, 15 Jan 2022 20:15:54: 4000000 INFO @ Sat, 15 Jan 2022 20:15:57: 13000000 INFO @ Sat, 15 Jan 2022 20:15:57: 21000000 INFO @ Sat, 15 Jan 2022 20:15:59: 5000000 INFO @ Sat, 15 Jan 2022 20:16:01: 14000000 INFO @ Sat, 15 Jan 2022 20:16:01: 22000000 INFO @ Sat, 15 Jan 2022 20:16:04: 6000000 INFO @ Sat, 15 Jan 2022 20:16:05: 15000000 INFO @ Sat, 15 Jan 2022 20:16:05: 23000000 INFO @ Sat, 15 Jan 2022 20:16:08: 7000000 INFO @ Sat, 15 Jan 2022 20:16:09: 16000000 INFO @ Sat, 15 Jan 2022 20:16:09: 24000000 INFO @ Sat, 15 Jan 2022 20:16:11: #1 tag size is determined as 26 bps INFO @ Sat, 15 Jan 2022 20:16:11: #1 tag size = 26 INFO @ Sat, 15 Jan 2022 20:16:11: #1 total tags in treatment: 5949534 INFO @ Sat, 15 Jan 2022 20:16:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:11: #1 tags after filtering in treatment: 4711204 INFO @ Sat, 15 Jan 2022 20:16:11: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 20:16:11: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:11: #2 number of paired peaks: 20 WARNING @ Sat, 15 Jan 2022 20:16:11: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:16:13: 17000000 INFO @ Sat, 15 Jan 2022 20:16:13: 8000000 INFO @ Sat, 15 Jan 2022 20:16:17: 18000000 INFO @ Sat, 15 Jan 2022 20:16:18: 9000000 INFO @ Sat, 15 Jan 2022 20:16:21: 19000000 INFO @ Sat, 15 Jan 2022 20:16:23: 10000000 INFO @ Sat, 15 Jan 2022 20:16:25: 20000000 INFO @ Sat, 15 Jan 2022 20:16:28: 11000000 INFO @ Sat, 15 Jan 2022 20:16:29: 21000000 INFO @ Sat, 15 Jan 2022 20:16:32: 12000000 INFO @ Sat, 15 Jan 2022 20:16:33: 22000000 INFO @ Sat, 15 Jan 2022 20:16:37: 13000000 INFO @ Sat, 15 Jan 2022 20:16:37: 23000000 INFO @ Sat, 15 Jan 2022 20:16:42: 24000000 INFO @ Sat, 15 Jan 2022 20:16:42: 14000000 INFO @ Sat, 15 Jan 2022 20:16:43: #1 tag size is determined as 26 bps INFO @ Sat, 15 Jan 2022 20:16:43: #1 tag size = 26 INFO @ Sat, 15 Jan 2022 20:16:43: #1 total tags in treatment: 5949534 INFO @ Sat, 15 Jan 2022 20:16:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:43: #1 tags after filtering in treatment: 4711204 INFO @ Sat, 15 Jan 2022 20:16:43: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 20:16:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:44: #2 number of paired peaks: 20 WARNING @ Sat, 15 Jan 2022 20:16:44: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 721 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:16:47: 15000000 INFO @ Sat, 15 Jan 2022 20:16:51: 16000000 INFO @ Sat, 15 Jan 2022 20:16:56: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:17:00: 18000000 INFO @ Sat, 15 Jan 2022 20:17:05: 19000000 INFO @ Sat, 15 Jan 2022 20:17:10: 20000000 INFO @ Sat, 15 Jan 2022 20:17:14: 21000000 INFO @ Sat, 15 Jan 2022 20:17:19: 22000000 INFO @ Sat, 15 Jan 2022 20:17:23: 23000000 INFO @ Sat, 15 Jan 2022 20:17:28: 24000000 INFO @ Sat, 15 Jan 2022 20:17:30: #1 tag size is determined as 26 bps INFO @ Sat, 15 Jan 2022 20:17:30: #1 tag size = 26 INFO @ Sat, 15 Jan 2022 20:17:30: #1 total tags in treatment: 5949534 INFO @ Sat, 15 Jan 2022 20:17:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:30: #1 tags after filtering in treatment: 4711204 INFO @ Sat, 15 Jan 2022 20:17:30: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 20:17:30: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:30: #2 number of paired peaks: 20 WARNING @ Sat, 15 Jan 2022 20:17:30: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 21 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245303/SRX8245303.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling