Job ID = 14520833 SRX = SRX8245302 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13394100 spots for SRR11684529/SRR11684529.sra Written 13394100 spots for SRR11684529/SRR11684529.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:40 13394100 reads; of these: 13394100 (100.00%) were paired; of these: 9260997 (69.14%) aligned concordantly 0 times 3596945 (26.85%) aligned concordantly exactly 1 time 536158 (4.00%) aligned concordantly >1 times ---- 9260997 pairs aligned concordantly 0 times; of these: 107911 (1.17%) aligned discordantly 1 time ---- 9153086 pairs aligned 0 times concordantly or discordantly; of these: 18306172 mates make up the pairs; of these: 9372786 (51.20%) aligned 0 times 7687379 (41.99%) aligned exactly 1 time 1246007 (6.81%) aligned >1 times 65.01% overall alignment rate Time searching: 00:06:40 Overall time: 00:06:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 106516 / 4240525 = 0.0251 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:12:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:12:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:12:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:12:22: 1000000 INFO @ Sat, 15 Jan 2022 20:12:26: 2000000 INFO @ Sat, 15 Jan 2022 20:12:30: 3000000 INFO @ Sat, 15 Jan 2022 20:12:34: 4000000 INFO @ Sat, 15 Jan 2022 20:12:38: 5000000 INFO @ Sat, 15 Jan 2022 20:12:42: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:12:46: 7000000 INFO @ Sat, 15 Jan 2022 20:12:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:12:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:12:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:12:50: 8000000 INFO @ Sat, 15 Jan 2022 20:12:52: 1000000 INFO @ Sat, 15 Jan 2022 20:12:55: 9000000 INFO @ Sat, 15 Jan 2022 20:12:57: 2000000 INFO @ Sat, 15 Jan 2022 20:12:59: 10000000 INFO @ Sat, 15 Jan 2022 20:13:01: 3000000 INFO @ Sat, 15 Jan 2022 20:13:04: 11000000 INFO @ Sat, 15 Jan 2022 20:13:06: 4000000 INFO @ Sat, 15 Jan 2022 20:13:08: 12000000 INFO @ Sat, 15 Jan 2022 20:13:10: 5000000 INFO @ Sat, 15 Jan 2022 20:13:12: 13000000 INFO @ Sat, 15 Jan 2022 20:13:15: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:13:17: 14000000 INFO @ Sat, 15 Jan 2022 20:13:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:13:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:13:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:13:19: 7000000 INFO @ Sat, 15 Jan 2022 20:13:22: 15000000 INFO @ Sat, 15 Jan 2022 20:13:23: 1000000 INFO @ Sat, 15 Jan 2022 20:13:24: 8000000 INFO @ Sat, 15 Jan 2022 20:13:26: 16000000 INFO @ Sat, 15 Jan 2022 20:13:28: 2000000 INFO @ Sat, 15 Jan 2022 20:13:29: 9000000 INFO @ Sat, 15 Jan 2022 20:13:31: 17000000 INFO @ Sat, 15 Jan 2022 20:13:32: #1 tag size is determined as 28 bps INFO @ Sat, 15 Jan 2022 20:13:32: #1 tag size = 28 INFO @ Sat, 15 Jan 2022 20:13:32: #1 total tags in treatment: 4027546 INFO @ Sat, 15 Jan 2022 20:13:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:13:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:13:32: #1 tags after filtering in treatment: 3369255 INFO @ Sat, 15 Jan 2022 20:13:32: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 20:13:32: #1 finished! INFO @ Sat, 15 Jan 2022 20:13:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:13:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:13:32: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 20:13:32: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:13:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:13:33: 10000000 INFO @ Sat, 15 Jan 2022 20:13:34: 3000000 INFO @ Sat, 15 Jan 2022 20:13:38: 11000000 INFO @ Sat, 15 Jan 2022 20:13:39: 4000000 INFO @ Sat, 15 Jan 2022 20:13:43: 12000000 INFO @ Sat, 15 Jan 2022 20:13:44: 5000000 INFO @ Sat, 15 Jan 2022 20:13:48: 13000000 INFO @ Sat, 15 Jan 2022 20:13:50: 6000000 INFO @ Sat, 15 Jan 2022 20:13:52: 14000000 INFO @ Sat, 15 Jan 2022 20:13:55: 7000000 INFO @ Sat, 15 Jan 2022 20:13:57: 15000000 INFO @ Sat, 15 Jan 2022 20:14:00: 8000000 INFO @ Sat, 15 Jan 2022 20:14:02: 16000000 INFO @ Sat, 15 Jan 2022 20:14:05: 9000000 INFO @ Sat, 15 Jan 2022 20:14:07: 17000000 INFO @ Sat, 15 Jan 2022 20:14:08: #1 tag size is determined as 28 bps INFO @ Sat, 15 Jan 2022 20:14:08: #1 tag size = 28 INFO @ Sat, 15 Jan 2022 20:14:08: #1 total tags in treatment: 4027546 INFO @ Sat, 15 Jan 2022 20:14:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:14:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:14:08: #1 tags after filtering in treatment: 3369255 INFO @ Sat, 15 Jan 2022 20:14:08: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 20:14:08: #1 finished! INFO @ Sat, 15 Jan 2022 20:14:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:14:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:14:08: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 20:14:08: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:14:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:14:10: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:14:15: 11000000 INFO @ Sat, 15 Jan 2022 20:14:20: 12000000 INFO @ Sat, 15 Jan 2022 20:14:25: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:14:30: 14000000 INFO @ Sat, 15 Jan 2022 20:14:35: 15000000 INFO @ Sat, 15 Jan 2022 20:14:40: 16000000 INFO @ Sat, 15 Jan 2022 20:14:44: 17000000 INFO @ Sat, 15 Jan 2022 20:14:45: #1 tag size is determined as 28 bps INFO @ Sat, 15 Jan 2022 20:14:45: #1 tag size = 28 INFO @ Sat, 15 Jan 2022 20:14:45: #1 total tags in treatment: 4027546 INFO @ Sat, 15 Jan 2022 20:14:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:14:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:14:45: #1 tags after filtering in treatment: 3369255 INFO @ Sat, 15 Jan 2022 20:14:45: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 20:14:45: #1 finished! INFO @ Sat, 15 Jan 2022 20:14:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:14:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:14:46: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 20:14:46: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:14:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245302/SRX8245302.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling