Job ID = 14520659 SRX = SRX8244968 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15325391 spots for SRR11684179/SRR11684179.sra Written 15325391 spots for SRR11684179/SRR11684179.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:58 15325391 reads; of these: 15325391 (100.00%) were unpaired; of these: 1761379 (11.49%) aligned 0 times 10633478 (69.38%) aligned exactly 1 time 2930534 (19.12%) aligned >1 times 88.51% overall alignment rate Time searching: 00:01:58 Overall time: 00:01:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5177067 / 13564012 = 0.3817 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:46:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:46:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:46:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:46:18: 1000000 INFO @ Sat, 15 Jan 2022 19:46:25: 2000000 INFO @ Sat, 15 Jan 2022 19:46:31: 3000000 INFO @ Sat, 15 Jan 2022 19:46:37: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:46:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:46:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:46:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:46:44: 5000000 INFO @ Sat, 15 Jan 2022 19:46:49: 1000000 INFO @ Sat, 15 Jan 2022 19:46:51: 6000000 INFO @ Sat, 15 Jan 2022 19:46:56: 2000000 INFO @ Sat, 15 Jan 2022 19:46:59: 7000000 INFO @ Sat, 15 Jan 2022 19:47:04: 3000000 INFO @ Sat, 15 Jan 2022 19:47:06: 8000000 INFO @ Sat, 15 Jan 2022 19:47:09: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:47:09: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:47:09: #1 total tags in treatment: 8386945 INFO @ Sat, 15 Jan 2022 19:47:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:09: #1 tags after filtering in treatment: 8386945 INFO @ Sat, 15 Jan 2022 19:47:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:47:09: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:09: Process for pairing-model is terminated! BedGraph に変換中... cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:47:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:47:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:47:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:47:11: 4000000 INFO @ Sat, 15 Jan 2022 19:47:18: 1000000 INFO @ Sat, 15 Jan 2022 19:47:19: 5000000 INFO @ Sat, 15 Jan 2022 19:47:25: 2000000 INFO @ Sat, 15 Jan 2022 19:47:27: 6000000 INFO @ Sat, 15 Jan 2022 19:47:32: 3000000 INFO @ Sat, 15 Jan 2022 19:47:34: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:47:39: 4000000 INFO @ Sat, 15 Jan 2022 19:47:42: 8000000 INFO @ Sat, 15 Jan 2022 19:47:45: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:47:45: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:47:45: #1 total tags in treatment: 8386945 INFO @ Sat, 15 Jan 2022 19:47:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:45: #1 tags after filtering in treatment: 8386945 INFO @ Sat, 15 Jan 2022 19:47:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:47:45: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:47:46: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:47:52: 6000000 INFO @ Sat, 15 Jan 2022 19:47:59: 7000000 INFO @ Sat, 15 Jan 2022 19:48:05: 8000000 INFO @ Sat, 15 Jan 2022 19:48:07: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:48:07: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:48:07: #1 total tags in treatment: 8386945 INFO @ Sat, 15 Jan 2022 19:48:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:48:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:48:07: #1 tags after filtering in treatment: 8386945 INFO @ Sat, 15 Jan 2022 19:48:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:48:07: #1 finished! INFO @ Sat, 15 Jan 2022 19:48:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:48:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:48:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:48:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:48:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling