Job ID = 10223998 SRX = SRX8244968 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15325391 spots for SRR11684179/SRR11684179.sra Written 15325391 spots for SRR11684179/SRR11684179.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:24 15325391 reads; of these: 15325391 (100.00%) were unpaired; of these: 1761379 (11.49%) aligned 0 times 10633478 (69.38%) aligned exactly 1 time 2930534 (19.12%) aligned >1 times 88.51% overall alignment rate Time searching: 00:01:24 Overall time: 00:01:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5177067 / 13564012 = 0.3817 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:07:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:07:53: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:07:53: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:07:58: 1000000 INFO @ Fri, 16 Oct 2020 09:08:03: 2000000 INFO @ Fri, 16 Oct 2020 09:08:08: 3000000 INFO @ Fri, 16 Oct 2020 09:08:13: 4000000 INFO @ Fri, 16 Oct 2020 09:08:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:08:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:08:23: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:08:23: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:08:23: 6000000 INFO @ Fri, 16 Oct 2020 09:08:29: 1000000 INFO @ Fri, 16 Oct 2020 09:08:29: 7000000 INFO @ Fri, 16 Oct 2020 09:08:35: 2000000 INFO @ Fri, 16 Oct 2020 09:08:35: 8000000 INFO @ Fri, 16 Oct 2020 09:08:37: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:08:37: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:08:37: #1 total tags in treatment: 8386945 INFO @ Fri, 16 Oct 2020 09:08:37: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:08:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:08:38: #1 tags after filtering in treatment: 8386945 INFO @ Fri, 16 Oct 2020 09:08:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:08:38: #1 finished! INFO @ Fri, 16 Oct 2020 09:08:38: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:08:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:08:38: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:08:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:08:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:08:41: 3000000 INFO @ Fri, 16 Oct 2020 09:08:47: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:08:53: 5000000 INFO @ Fri, 16 Oct 2020 09:08:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:08:53: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:08:53: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:08:59: 6000000 INFO @ Fri, 16 Oct 2020 09:09:00: 1000000 INFO @ Fri, 16 Oct 2020 09:09:05: 7000000 INFO @ Fri, 16 Oct 2020 09:09:07: 2000000 INFO @ Fri, 16 Oct 2020 09:09:11: 8000000 INFO @ Fri, 16 Oct 2020 09:09:13: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:09:13: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:09:13: #1 total tags in treatment: 8386945 INFO @ Fri, 16 Oct 2020 09:09:13: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:09:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:09:13: #1 tags after filtering in treatment: 8386945 INFO @ Fri, 16 Oct 2020 09:09:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:09:13: #1 finished! INFO @ Fri, 16 Oct 2020 09:09:13: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:09:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:09:14: 3000000 INFO @ Fri, 16 Oct 2020 09:09:14: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:09:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:09:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:09:20: 4000000 INFO @ Fri, 16 Oct 2020 09:09:26: 5000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 09:09:33: 6000000 INFO @ Fri, 16 Oct 2020 09:09:39: 7000000 INFO @ Fri, 16 Oct 2020 09:09:45: 8000000 INFO @ Fri, 16 Oct 2020 09:09:47: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:09:47: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:09:47: #1 total tags in treatment: 8386945 INFO @ Fri, 16 Oct 2020 09:09:47: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:09:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:09:48: #1 tags after filtering in treatment: 8386945 INFO @ Fri, 16 Oct 2020 09:09:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:09:48: #1 finished! INFO @ Fri, 16 Oct 2020 09:09:48: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:09:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:09:48: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:09:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:09:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244968/SRX8244968.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling