Job ID = 14520658 SRX = SRX8244967 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15733094 spots for SRR11684178/SRR11684178.sra Written 15733094 spots for SRR11684178/SRR11684178.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:40 15733094 reads; of these: 15733094 (100.00%) were unpaired; of these: 895321 (5.69%) aligned 0 times 12077860 (76.77%) aligned exactly 1 time 2759913 (17.54%) aligned >1 times 94.31% overall alignment rate Time searching: 00:01:40 Overall time: 00:01:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5322837 / 14837773 = 0.3587 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:37:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:37:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:37:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:37:34: 1000000 INFO @ Sat, 15 Jan 2022 19:37:39: 2000000 INFO @ Sat, 15 Jan 2022 19:37:44: 3000000 INFO @ Sat, 15 Jan 2022 19:37:49: 4000000 INFO @ Sat, 15 Jan 2022 19:37:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:37:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:37:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:37:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:38:00: 6000000 INFO @ Sat, 15 Jan 2022 19:38:05: 1000000 INFO @ Sat, 15 Jan 2022 19:38:06: 7000000 INFO @ Sat, 15 Jan 2022 19:38:11: 2000000 INFO @ Sat, 15 Jan 2022 19:38:13: 8000000 INFO @ Sat, 15 Jan 2022 19:38:17: 3000000 INFO @ Sat, 15 Jan 2022 19:38:19: 9000000 INFO @ Sat, 15 Jan 2022 19:38:21: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:38:21: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:38:21: #1 total tags in treatment: 9514936 INFO @ Sat, 15 Jan 2022 19:38:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:38:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:38:22: #1 tags after filtering in treatment: 9514936 INFO @ Sat, 15 Jan 2022 19:38:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:38:22: #1 finished! INFO @ Sat, 15 Jan 2022 19:38:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:38:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:38:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:38:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:38:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 19:38:22: 4000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:38:28: 5000000 INFO @ Sat, 15 Jan 2022 19:38:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:38:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:38:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:38:34: 6000000 INFO @ Sat, 15 Jan 2022 19:38:35: 1000000 INFO @ Sat, 15 Jan 2022 19:38:41: 7000000 INFO @ Sat, 15 Jan 2022 19:38:41: 2000000 INFO @ Sat, 15 Jan 2022 19:38:46: 8000000 INFO @ Sat, 15 Jan 2022 19:38:47: 3000000 INFO @ Sat, 15 Jan 2022 19:38:53: 9000000 INFO @ Sat, 15 Jan 2022 19:38:53: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:38:56: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:38:56: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:38:56: #1 total tags in treatment: 9514936 INFO @ Sat, 15 Jan 2022 19:38:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:38:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:38:57: #1 tags after filtering in treatment: 9514936 INFO @ Sat, 15 Jan 2022 19:38:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:38:57: #1 finished! INFO @ Sat, 15 Jan 2022 19:38:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:38:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:38:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:38:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:38:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:39:00: 5000000 INFO @ Sat, 15 Jan 2022 19:39:05: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:39:10: 7000000 INFO @ Sat, 15 Jan 2022 19:39:15: 8000000 INFO @ Sat, 15 Jan 2022 19:39:20: 9000000 INFO @ Sat, 15 Jan 2022 19:39:22: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:39:22: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:39:22: #1 total tags in treatment: 9514936 INFO @ Sat, 15 Jan 2022 19:39:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:39:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:39:23: #1 tags after filtering in treatment: 9514936 INFO @ Sat, 15 Jan 2022 19:39:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:39:23: #1 finished! INFO @ Sat, 15 Jan 2022 19:39:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:39:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:39:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:39:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:39:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244967/SRX8244967.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling