Job ID = 10223996 SRX = SRX8244966 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 17662242 spots for SRR11684177/SRR11684177.sra Written 17662242 spots for SRR11684177/SRR11684177.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:42 17662242 reads; of these: 17662242 (100.00%) were unpaired; of these: 1698396 (9.62%) aligned 0 times 12537767 (70.99%) aligned exactly 1 time 3426079 (19.40%) aligned >1 times 90.38% overall alignment rate Time searching: 00:01:42 Overall time: 00:01:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6314154 / 15963846 = 0.3955 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:08:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:08:02: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:08:02: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:08:07: 1000000 INFO @ Fri, 16 Oct 2020 09:08:12: 2000000 INFO @ Fri, 16 Oct 2020 09:08:17: 3000000 INFO @ Fri, 16 Oct 2020 09:08:22: 4000000 INFO @ Fri, 16 Oct 2020 09:08:27: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:08:32: 6000000 INFO @ Fri, 16 Oct 2020 09:08:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:08:32: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:08:32: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:08:37: 7000000 INFO @ Fri, 16 Oct 2020 09:08:38: 1000000 INFO @ Fri, 16 Oct 2020 09:08:43: 8000000 INFO @ Fri, 16 Oct 2020 09:08:43: 2000000 INFO @ Fri, 16 Oct 2020 09:08:48: 9000000 INFO @ Fri, 16 Oct 2020 09:08:48: 3000000 INFO @ Fri, 16 Oct 2020 09:08:51: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:08:51: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:08:51: #1 total tags in treatment: 9649692 INFO @ Fri, 16 Oct 2020 09:08:51: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:08:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:08:51: #1 tags after filtering in treatment: 9649692 INFO @ Fri, 16 Oct 2020 09:08:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:08:51: #1 finished! INFO @ Fri, 16 Oct 2020 09:08:51: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:08:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:08:52: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:08:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:08:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:08:54: 4000000 INFO @ Fri, 16 Oct 2020 09:08:59: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:09:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:09:02: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:09:02: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:09:04: 6000000 INFO @ Fri, 16 Oct 2020 09:09:09: 1000000 INFO @ Fri, 16 Oct 2020 09:09:09: 7000000 INFO @ Fri, 16 Oct 2020 09:09:15: 2000000 INFO @ Fri, 16 Oct 2020 09:09:15: 8000000 INFO @ Fri, 16 Oct 2020 09:09:21: 9000000 INFO @ Fri, 16 Oct 2020 09:09:21: 3000000 INFO @ Fri, 16 Oct 2020 09:09:24: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:09:24: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:09:24: #1 total tags in treatment: 9649692 INFO @ Fri, 16 Oct 2020 09:09:24: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:09:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:09:24: #1 tags after filtering in treatment: 9649692 INFO @ Fri, 16 Oct 2020 09:09:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:09:24: #1 finished! INFO @ Fri, 16 Oct 2020 09:09:24: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:09:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:09:25: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:09:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:09:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:09:27: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:09:32: 5000000 INFO @ Fri, 16 Oct 2020 09:09:38: 6000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 09:09:44: 7000000 INFO @ Fri, 16 Oct 2020 09:09:50: 8000000 INFO @ Fri, 16 Oct 2020 09:09:56: 9000000 INFO @ Fri, 16 Oct 2020 09:10:00: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:10:00: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:10:00: #1 total tags in treatment: 9649692 INFO @ Fri, 16 Oct 2020 09:10:00: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:10:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:10:00: #1 tags after filtering in treatment: 9649692 INFO @ Fri, 16 Oct 2020 09:10:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:10:00: #1 finished! INFO @ Fri, 16 Oct 2020 09:10:00: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:10:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:10:00: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:10:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:10:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244966/SRX8244966.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling