Job ID = 14520656 SRX = SRX8244965 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19208462 spots for SRR11684176/SRR11684176.sra Written 19208462 spots for SRR11684176/SRR11684176.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:06 19208462 reads; of these: 19208462 (100.00%) were unpaired; of these: 1472873 (7.67%) aligned 0 times 14289171 (74.39%) aligned exactly 1 time 3446418 (17.94%) aligned >1 times 92.33% overall alignment rate Time searching: 00:03:06 Overall time: 00:03:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7182160 / 17735589 = 0.4050 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:39:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:39:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:39:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:39:36: 1000000 INFO @ Sat, 15 Jan 2022 19:39:42: 2000000 INFO @ Sat, 15 Jan 2022 19:39:47: 3000000 INFO @ Sat, 15 Jan 2022 19:39:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:39:58: 5000000 INFO @ Sat, 15 Jan 2022 19:40:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:40:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:40:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:40:03: 6000000 INFO @ Sat, 15 Jan 2022 19:40:06: 1000000 INFO @ Sat, 15 Jan 2022 19:40:09: 7000000 INFO @ Sat, 15 Jan 2022 19:40:13: 2000000 INFO @ Sat, 15 Jan 2022 19:40:15: 8000000 INFO @ Sat, 15 Jan 2022 19:40:19: 3000000 INFO @ Sat, 15 Jan 2022 19:40:21: 9000000 INFO @ Sat, 15 Jan 2022 19:40:24: 4000000 INFO @ Sat, 15 Jan 2022 19:40:26: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:40:30: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:40:30: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:40:30: #1 total tags in treatment: 10553429 INFO @ Sat, 15 Jan 2022 19:40:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:40:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:40:30: 5000000 INFO @ Sat, 15 Jan 2022 19:40:30: #1 tags after filtering in treatment: 10553429 INFO @ Sat, 15 Jan 2022 19:40:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:40:30: #1 finished! INFO @ Sat, 15 Jan 2022 19:40:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:40:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:40:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:40:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:40:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:40:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:40:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:40:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:40:35: 6000000 INFO @ Sat, 15 Jan 2022 19:40:36: 1000000 INFO @ Sat, 15 Jan 2022 19:40:41: 7000000 INFO @ Sat, 15 Jan 2022 19:40:42: 2000000 INFO @ Sat, 15 Jan 2022 19:40:46: 8000000 INFO @ Sat, 15 Jan 2022 19:40:47: 3000000 INFO @ Sat, 15 Jan 2022 19:40:52: 9000000 INFO @ Sat, 15 Jan 2022 19:40:53: 4000000 INFO @ Sat, 15 Jan 2022 19:40:57: 10000000 INFO @ Sat, 15 Jan 2022 19:40:58: 5000000 INFO @ Sat, 15 Jan 2022 19:41:00: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:41:00: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:41:00: #1 total tags in treatment: 10553429 INFO @ Sat, 15 Jan 2022 19:41:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:41:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:41:00: #1 tags after filtering in treatment: 10553429 INFO @ Sat, 15 Jan 2022 19:41:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:41:00: #1 finished! INFO @ Sat, 15 Jan 2022 19:41:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:41:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:41:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:41:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:41:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:41:03: 6000000 INFO @ Sat, 15 Jan 2022 19:41:08: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:41:13: 8000000 INFO @ Sat, 15 Jan 2022 19:41:18: 9000000 INFO @ Sat, 15 Jan 2022 19:41:23: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:41:25: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:41:25: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:41:25: #1 total tags in treatment: 10553429 INFO @ Sat, 15 Jan 2022 19:41:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:41:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:41:26: #1 tags after filtering in treatment: 10553429 INFO @ Sat, 15 Jan 2022 19:41:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:41:26: #1 finished! INFO @ Sat, 15 Jan 2022 19:41:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:41:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:41:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:41:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:41:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244965/SRX8244965.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling