Job ID = 10223993 SRX = SRX8244964 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 16732764 spots for SRR11684175/SRR11684175.sra Written 16732764 spots for SRR11684175/SRR11684175.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:21 16732764 reads; of these: 16732764 (100.00%) were unpaired; of these: 5535378 (33.08%) aligned 0 times 8930784 (53.37%) aligned exactly 1 time 2266602 (13.55%) aligned >1 times 66.92% overall alignment rate Time searching: 00:01:21 Overall time: 00:01:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 4825838 / 11197386 = 0.4310 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:06:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:06:32: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:06:32: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:06:36: 1000000 INFO @ Fri, 16 Oct 2020 09:06:41: 2000000 INFO @ Fri, 16 Oct 2020 09:06:45: 3000000 INFO @ Fri, 16 Oct 2020 09:06:49: 4000000 INFO @ Fri, 16 Oct 2020 09:06:53: 5000000 INFO @ Fri, 16 Oct 2020 09:06:57: 6000000 INFO @ Fri, 16 Oct 2020 09:06:59: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:06:59: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:06:59: #1 total tags in treatment: 6371548 INFO @ Fri, 16 Oct 2020 09:06:59: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:06:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:06:59: #1 tags after filtering in treatment: 6371548 INFO @ Fri, 16 Oct 2020 09:06:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:06:59: #1 finished! INFO @ Fri, 16 Oct 2020 09:06:59: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:06:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:06:59: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:06:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:06:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:07:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:07:02: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:07:02: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:07:06: 1000000 INFO @ Fri, 16 Oct 2020 09:07:11: 2000000 INFO @ Fri, 16 Oct 2020 09:07:15: 3000000 INFO @ Fri, 16 Oct 2020 09:07:19: 4000000 INFO @ Fri, 16 Oct 2020 09:07:23: 5000000 INFO @ Fri, 16 Oct 2020 09:07:28: 6000000 INFO @ Fri, 16 Oct 2020 09:07:29: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:07:29: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:07:29: #1 total tags in treatment: 6371548 INFO @ Fri, 16 Oct 2020 09:07:29: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:07:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:07:29: #1 tags after filtering in treatment: 6371548 INFO @ Fri, 16 Oct 2020 09:07:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:07:29: #1 finished! INFO @ Fri, 16 Oct 2020 09:07:29: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:07:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:07:30: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:07:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:07:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:07:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:07:32: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:07:32: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:07:36: 1000000 INFO @ Fri, 16 Oct 2020 09:07:40: 2000000 INFO @ Fri, 16 Oct 2020 09:07:44: 3000000 INFO @ Fri, 16 Oct 2020 09:07:49: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:07:53: 5000000 INFO @ Fri, 16 Oct 2020 09:07:57: 6000000 INFO @ Fri, 16 Oct 2020 09:07:59: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:07:59: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:07:59: #1 total tags in treatment: 6371548 INFO @ Fri, 16 Oct 2020 09:07:59: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:07:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:07:59: #1 tags after filtering in treatment: 6371548 INFO @ Fri, 16 Oct 2020 09:07:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:07:59: #1 finished! INFO @ Fri, 16 Oct 2020 09:07:59: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:07:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:07:59: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:07:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:07:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244964/SRX8244964.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。